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Active Matrix Metalloproteinase 7 (MMP7)
MPSL1; MAT; PUMP-1; Matrilysin Uterine; Matrilysin; Matrin; Pump-1 Protease; Uterine Metalloproteinase
- Product No.APA102Hu61
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
- Traits Freeze-dried powder
- Purity> 95%
- Isoelectric Point7.8
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
- FOB
US$ 320
US$ 800
US$ 1600
US$ 4800
US$ 12000
For more details, please contact local distributors!
ACTIVITY TEST
Matrix Metalloproteinase 7 (MMP7) is a member of the matrix metalloproteinases (MMPs) family which are zinc and calcium dependent endopeptidases. Structurally, MMP-7 is the smallest of the MMPs and consists of two domains: a pro-domain that is cleaved upon activation and a catalytic domain containing the zinc-binding site. MMP-7 (matrilysin) is expressed in epithelial cells of normal and diseased tissues, and is capable of digesting a large series of proteins of the extracellular matrix including collagen IV and X, gelatin, casein, laminin, aggrecan, entactin, elastin and versican. Thus we have chosen casein-zymography to measure the activity of MMP7. Briefly, various concentrations of MMP7 (10ug, 5ug, 1ug, 0.1ug ,0.01ug) were denatured by SDS loading buffer, electrophoresed through sodium dodecylsulphat-polyacrylamide gel (SDS-PAGE; 15% gels) containing casein (1 mg/ml) with nonreducing conditions. After renaturation, incubation and CCB-stained, active MMP7 would hydrolyze casein nearby, which was indicated by the white bands on the gel. In this experiment, we use heat-denatured MMP7 protein as negative control, and trypsin (1ug/ml) as positive control. The result was shown in figure 1.
USAGE
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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| Magazine | Citations |
| British Journal of Pharmacology | A novel, orally active LPA1 receptor antagonist inhibits lung fibrosis in the mouse bleomycin model PubMed: 20649573 |
| Neuroimmunomodulation | Upregulated Expression of Matrix Metalloproteinases and Tissue Inhibitors of Matrix Metalloproteinases in BALB/c Mouse Brain Challenged with Japanese Encephalitis Virus Pubmed: source |
| PLoS ONE | A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches Plosone: Source |
| Thromb Haemost. | Analysis of the expression of nine secreted matrix metalloproteinases and their endogenous inhibitors in the brain of mice subjected to ischaemic stroke Pubmed:24671655 |
| Histol Histopathol | Estrogen-deficient osteoporosis enhances the recruitment and activity of osteoclasts by breast cancer cells PubMed: 26254457 |
| VirusDisease | Circulating levels of matrix metalloproteinases and tissue inhibitors of matrix metalloproteinases during Japanese encephalitis virus infection Pubmed:26925446 |
| Am J Physiol Lung Cell Mol Physiol | Systematic phenotyping and correlation of biomarkers with lung function and histology in lung fibrosis Pubmed:26993522 |
| Cellular Physiology and Biochemistry | The MiR-495/Annexin A3/P53 Axis Inhibits the Invasion and EMT of Colorectal Cancer Cells pubmed:29224019 |
| s-space | Thyroid disrupting effects and associated mechanisms of TCPP in GH3 cell line and zebrafish (Danio rerio) larva and adult 10371/137708 |
| Science Translational Medicine | Large-scale proteomics identifies MMP-7 as a sentinel of epithelial injury and of biliary atresia. pubmed:29167395 |
| Hepatology | Diagnostic Accuracy of Serum Matrix Metalloproteinase‐7 for Biliary Atresia Pubmed: 30153340 |
| Journal of Pediatrics | Quantification of Serum Matrix Metallopeptide 7 Levels May Assist in the Diagnosis and Predict the Outcome for Patients with Biliary Atresia Pubmed: 30853207 |
| Pediatrics | Serum MMP-7 in the Diagnosis of Biliary Atresia Pubmed: 31604829 |
| Biomed Pharmacother | Human umbilical cord blood mononuclear cells protect against renal tubulointerstitial fibrosis in cisplatin-treated rats Pubmed: 31710895 |
| LABORATORY INVESTIGATION | The synthetic toxin biliatresone causes biliary atresia in mice Pubmed: 32681026 |
| diagnostics | Interstitial Score and Concentrations of IL-4R¦Á, PAR-2, and MMP-7 in Bronchoalveolar Lavage Fluid Could Be Useful Markers for Distinguishing Idiopathic Interstitial?¡ 33924683 |
| Cancer Cell Int | A novel strategy to identify candidate diagnostic and prognostic biomarkers for gastric cancer 34215253 |