≡

Active Prostate Specific Antigen (PSA)

KLK3; APS; KLK2A1; HK3; KLKB1; Seminin; Prostate Specific Antigen; Kallikrein-Related Peptidase 3; Semenogelase, g-Seminoprotein; P-30 Antigen; Plasma kallikrein

ACTIVITY TEST

Kallikrein 3, commonly known as prostate specific antigen (PSA), is a serine protease of the human tissue Kallikrein gene family. PSA is synthesized in the ductal and acinar epithelium of the prostate gland and secreted into the seminal plasma in high concentrations (0.5 - 2 g/L). A small portion of PSA “leaks” into the systemic circulation, the levels of which increase significantly (30-fold) from prostate cancer tissue than normal prostate tissue. PSA has become a well established tumor marker that aids the diagnosis, staging, and follow up of prostate cancer. The activity of recombinant human KLK3 is measured by its ability to cleave a colorimetric peptide substrate Suc-Arg-Pro-Tyr-pNa in the assay buffer 50 mM Tris, 1 M NaCl, pH 8.0. The rhKLK3 is diluted to 200 ug/ml in activation buffer 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% Brij-35, pH 7.5, then activated with a final concentration of 1 ug/ml Thermolysin at 37 ℃ for 5min. Adding a final concentration of 10 mM 1,10 Phenanthroline to stop the activation. The activated rhKLK3 is diluted to 50 ug/mL in assay buffer. Loading into a clear well plate 50 µL of 50 ug/mL rhKLK3 and start the reaction by adding 50 µL of 2 mM substrate, with a substrate blank containing 50 µL assay buffer, 50 µL substrate, and no rhKLK3. Then read at 405 nm in kinetic mode for 5 minutes. The specific activity of recombinant human KLK3 is > 400 pmol/min/µg.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
The Journal of Nutritional Biochemistry Vitamin C supplementation prevents testosterone-induced hyperplasia of rat prostate by down-regulating HIF-1α ScienceDirect: S0955286309001181
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
EPA151Hu61 Eukaryotic Prostate Specific Antigen (PSA) Positive Control; Immunogen; SDS-PAGE; WB.
APA151Hu01 Active Prostate Specific Antigen (PSA) Cell culture; Activity Assays.
RPA151Hu01 Recombinant Prostate Specific Antigen (PSA) Positive Control; Immunogen; SDS-PAGE; WB.
APA151Hu61 Active Prostate Specific Antigen (PSA) Cell culture; Activity Assays.
RPA151Hu02 Recombinant Prostate Specific Antigen (PSA) Positive Control; Immunogen; SDS-PAGE; WB.
CPA151Hu21 OVA Conjugated Prostate Specific Antigen (PSA) Immunogen; SDS-PAGE; WB.
PAA151Hu01 Polyclonal Antibody to Prostate Specific Antigen (PSA) WB; IHC; ICC; IP.
PAA151Hu02 Polyclonal Antibody to Prostate Specific Antigen (PSA) WB; IHC; ICC; IP.
MAA151Hu23 Monoclonal Antibody to Prostate Specific Antigen (PSA) WB; IHC; ICC; IP.
MAA151Hu24 Monoclonal Antibody to Prostate Specific Antigen (PSA) WB
MAA151Hu21 Monoclonal Antibody to Prostate Specific Antigen (PSA) WB; IHC; ICC; IP.
MAA151Hu22 Monoclonal Antibody to Prostate Specific Antigen (PSA) WB; IHC; ICC; IP.
SEA151Hu ELISA Kit for Prostate Specific Antigen (PSA) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA151Hu CLIA Kit for Prostate Specific Antigen (PSA) Chemiluminescent immunoassay for Antigen Detection.
LMA151Hu Multiplex Assay Kit for Prostate Specific Antigen (PSA) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA151Hu01 ELISA Kit DIY Materials for Prostate Specific Antigen (PSA) Main materials for "Do It (ELISA Kit) Yourself".