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Active Granzyme M (GZMM)

GZM-M; LMET1; MET1; Hu-Met-1; Met-ase; Lymphocyte Met Ase 1; Met-1 serine protease; Natural killer cell granular protease

ACTIVITY TEST

GZMM (Granzyme M) is one of the neutral serine proteases, which is specifically expressed by NK cells and mediates a novel major and perforin-dependent cell death pathway. Granzyme M has been proven to targets a-Tubulin and disorganizes the microtubule network, besides, Ezrin has also been identified as a substrate of GZMM. Human granzyme M is synthesized as a precursor (264 residues) with a signal peptide (residues 123), a propeptide (residues 24-25) and a mature chain (residues 26-257). The purified recombinant human Granzyme M consists of residues 26 to 257 which activity was measured by its ability to cleaves a thioester substrate Z-Lys-SBzl•HCl. The reaction was performed in 0.05 M Tris, 0.15 M NaCl, 0.01% Triton X-100, pH 8.0 (assay buffer), initiated by addition 50 μL of various concentrations of GZMM (diluted by assay buffer) to 50 µL of 1.2 mM substrate and DTNB mixture. The final well serves as a negative control with no GZMM, replace with 50 μL assay buffer. Incubated at 25℃ for 5min, then read at a wavelength of 405 nm. The specific activity of recombinant human Granzyme M is >80 pmol/min/µg.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Clinical Immunology Elevated granzyme M-expressing lymphocytes during cytomegalovirus latency and reactivation after allogeneic stem cell transplantation ScienceDirect: S1521661613002970
Immunobiology. Granzyme M and K release in human experimental endotoxemia science:S0171298516300225
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPA431Hu01 Recombinant Granzyme M (GZMM) Positive Control; Immunogen; SDS-PAGE; WB.
APA431Hu01 Active Granzyme M (GZMM) Cell culture; Activity Assays.
PAA431Hu01 Polyclonal Antibody to Granzyme M (GZMM) WB; IHC
LAA431Hu71 Biotin-Linked Polyclonal Antibody to Granzyme M (GZMM) WB; IHC; ICC.
LAA431Hu81 FITC-Linked Polyclonal Antibody to Granzyme M (GZMM) WB; IHC; ICC; IF.
MAA431Hu23 Monoclonal Antibody to Granzyme M (GZMM) WB; IHC; ICC; IP.
MAA431Hu22 Monoclonal Antibody to Granzyme M (GZMM) WB,IHC
SEA431Hu ELISA Kit for Granzyme M (GZMM) Enzyme-linked immunosorbent assay for Antigen Detection.