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Active P-Selectin (SELP)

CD62P; Selectin, Platelet; PSEL; GMP140; LECAM3; GRMP; GMRP; PADGEM; Leukocyte-endothelial cell adhesion molecule 3; Platelet activation dependent granule-external membrane

ACTIVITY TEST

Selectin (SELP), a Ca(2 )-dependent receptor on myeloid cells, belongs to the selectin/LECAM family. SELP binds to neutrophils and monocytes via carbohydrates, it interacts with SELPLG to enable rapid leukocyte rolling over vascular surfaces in early inflammation. It has been reported that that CD24 is a ligand for P-selectin and the CD24/P-selectin binding pathway could be important in the dissemination of tumor cells by facilitating the interaction with platelets or endothelial cells. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human SELP and recombinant human CD24. Briefly, biotin-linked SELP were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to CD24-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µl stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant human SELP and recombinant human CD24 was shown in Figure 1, the EC50 for this effect is 0.56 ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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INCREMENT SERVICES

Magazine Citations
Journalof Vascular Research Effect of Hirulog-Like Peptide on Balloon Catheter Injury-Induced Neointimal Formation in Femoral Arteries of Minipigs and Relationship with Inflammatory Mediators Karger: 000257340
Basic Research in Cardiology Secretome of apoptotic peripheral blood cells (APOSEC) attenuates microvascular obstruction in a porcine closed chest reperfused acute myocardial infarction model: role of platelet aggregation and vasodilation PubMed: 22899170
BioMed research international Endothelial gene expression and molecular changes in response to radiosurgery in in vitro and in vivo models of cerebral arteriovenous malformations Pubmed: 24199192
Free Radical Biology and Medicine Involvement of nitric oxide with activation of Toll-like receptor 4 signaling in mice with dextran sodium sulfate-induced colitis Pubmed:24992835
Materials Science and Engineering: C MRI-compatible Nb–60Ta–2Zr alloy used for vascular stents: Haemocompatibility and its correlation with protein adsorption Pubmed:25063132
Blood Coagul Fibrinolysis.? Chemical sympathectomy attenuates inflammation, glycocalyx shedding and coagulation disorders in rats with acute traumatic coagulopathy. Pubmed:25325345
Inflammation.? Systemic and Flap Inflammatory Response Associates with Thrombosis in Flap Venous Crisis Pubmed:25448261
Endocrine Effects of glucagon-like peptide-1 on advanced glycation endproduct-induced aortic endothelial dysfunction in streptozotocin-induced diabetic rats: possible roles of Rho kinase- and AMP kinase-mediated nuclear factor κB signaling pathways Pubmed:26758998
Microbial Pathogenesis Protective effects of sinomenine against LPS-induced inflammation in piglets. pubmed:28757275
Nutrition & Metabolism L-Carnitine Supplementation Increases Trimethylamine-N-Oxide but not Markers of Atherosclerosis in Healthy Aged Women Pubmed: 30485835
Available at SSRN 3335365  Evaluating the Platelet Activation Related to the Degradation of Biomaterials by Scheme of Molecular Markers
Ecotoxicology and Environmental Safety Molecular mechanisms of lead-induced changes of selenium status in mice livers through interacting with selenoprotein P
Thrombosis Research Feasibility study of use of rabbit blood to evaluate platelet activation by medical devices Pubmed: 31838449
JOURNAL OF CONTROLLED RELEASE Polycaprolactone vascular graft with epigallocatechin gallate embedded sandwiched layer-by-layer functionalization for enhanced antithrombogenicity and anti … Pubmed: 31982435
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY The Inhibition of P-Selectin Reduced Severe Acute Lung Injury in Immunocompromised Mice Pubmed: 32377309
blood advances The binding of autotaxin to integrins mediates hyperhomocysteinemia-potentiated platelet activation and thrombosis 34559203
Vaccine The effect of ChAdOx1 nCov-19 vaccine on arterial thrombosis development and platelet aggregation in female rats Pubmed:35183388
Int J Biol Macromol The inhibition of TRIM35-mediated TIGAR ubiquitination enhances mitochondrial fusion and alleviates renal ischemia-reperfusion injury Pubmed:35421414
Experimental and Therapeutic Medicine Construction of a rabbit model with vinorelbine administration via peripherally inserted central catheter and dynamic monitoring of changes in phlebitis and thrombosis Pubmed:35126715
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPA569Hu02 Recombinant P-Selectin (SELP) Positive Control; Immunogen; SDS-PAGE; WB.
EPA569Hu61 Eukaryotic P-Selectin (SELP) Positive Control; Immunogen; SDS-PAGE; WB.
APA569Hu61 Active P-Selectin (SELP) Cell culture; Activity Assays.
RPA569Hu03 Recombinant P-Selectin (SELP) Positive Control; Immunogen; SDS-PAGE; WB.
RPA569Hu01 Recombinant P-Selectin (SELP) Positive Control; Immunogen; SDS-PAGE; WB.
PAA569Hu01 Polyclonal Antibody to P-Selectin (SELP) WB; IHC; ICC; IP.
PAA569Hu02 Polyclonal Antibody to P-Selectin (SELP) WB; IHC; ICC; IP.
FAA569Hu01 Anti-P-Selectin (SELP) Polyclonal Antibody Flow cytometry.
LAA569Hu71 Biotin-Linked Polyclonal Antibody to P-Selectin (SELP) WB; IHC; ICC.
LAA569Hu81 FITC-Linked Polyclonal Antibody to P-Selectin (SELP) WB; IHC; ICC; IF.
MAA569Hu24 Monoclonal Antibody to P-Selectin (SELP) WB; IHC; ICC; IP.
MAA569Hu21 Monoclonal Antibody to P-Selectin (SELP) WB; IHC; ICC; IP.
MAA569Hu22 Monoclonal Antibody to P-Selectin (SELP) WB; IHC; ICC; IP.
MAA569Hu23 Monoclonal Antibody to P-Selectin (SELP) WB; IHC; ICC; IP.
SEA569Hu ELISA Kit for P-Selectin (SELP) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA569Hu CLIA Kit for P-Selectin (SELP) Chemiluminescent immunoassay for Antigen Detection.
LMA569Hu Multiplex Assay Kit for P-Selectin (SELP) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.