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Active Glypican 3 (GPC3)

DGSX; OCI5; SDYS; SGB; SGBS1; MXR7; Glypican Proteoglycan 3; GTR2-2; Intestinal protein OCI-5; Secreted glypican-3

ACTIVITY TEST

Glypican-3 (GPC3), a 70 kDa protein, is a member of the glypican family that attaches to the cell surface by a glycosylphosphatidylinositol anchor, is specifically up-regulated in hepatocellular carcinoma (HCC) although rarely or not expressed in normal liver tissues, making it a perfect diagnostic and treatment target for HCC. GPC3 is also a negative transcriptional regulator and tumor suppressor that inhibits the growth of breast, ovary, and lung cancer cells. It is reported that GPC3 can form a complex with insulin-like growth factor 2 (IGF2), and might thereby modulate IGF2 action. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human GPC3 and recombinant rat IGF2. Briefly, GPC3 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to IGF2-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-GPC3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human GPC3 and recombinant rat IGF2 was shown in Figure 1, the EC50 for this effect is 0.08 ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Nature and Science Diagnostic Role of Serum Glypican-3 as a Tumor Marker for Hepatocellular Carcinoma Sciencepub:10043238
Journal of Applied Pharmaceutical Science Cytotoxic effects of antiglypican-3 against HepG2 cell line Ebscohost: Source
Asian Pacific Journal of Cancer Prevention Can Glypican3 be Diagnostic for Early Hepatocellular Carcinoma among Egyptian Patients? Pubmed: 24460300
Clin Biochem. Diagnostic accuracy of serum glypican-3 for hepatocellular carcinoma: A systematic review and meta-analysis Pubmed: 24362268
Science and Nature Diagnostic Role of Serum Glypican-3 as Hepatocellular Carcinoma Zolcat: Source
Alexandria Journal of Medicine Role of serum glypican-3 in the diagnosis and differentiation of small hepatocellular carcinoma from hepatitis-C virus cirrhosis ScienceDirect: S2090506814000062
J Egypt Natl Canc Inst. Comparing prothrombin induced by vitamin K absence-II (PIVKA-II) with the oncofetal proteins glypican-3, Alpha feto protein and carcinoembryonic antigen in diagnosing hepatocellular carcinoma among Egyptian patients. Pubmed:24841158
European Journal of Pharmacology Evaluation of antiglypican-3 therapy as a promising target for amelioration of hepatic tissue damage in hepatocellular carcinoma Pubmed:25449037
Hepatobiliary Pancreat Dis Int Predictive value of tumor markers in patients with recurrent hepatocellular carcinoma in different vascular invasion pattern abstract:abstract4525.shtml
Clinical and Molecular Hepatology  Glypican-3 level assessed by the enzyme-linked immunosorbent assay is inferior to alpha-fetoprotein level for hepatocellular carcinoma diagnosis pmc:articles
Wiley Glypican‐3: A promising biomarker for hepatocellular carcinoma diagnosis and treatment pubmed:28621802
Biomedicine & Pharmacotherapy Cytotoxic and partial hepatoprotective activity of sodium ascorbate against hepatocellular carcinoma through inhibition of sulfatase-2 and Pubmed:29669302
Journal of Cancer and Clinical Oncology Development and Validation of a Two-Site Immunoradiometric assay for Glypican-3 in plasma: Implication in Diagnosis of Hepatocellular Carcinoma Researchgate:Source
Turkish journal of oncology Serum Glypican-3, Vascular Endothelial Growth Factor, and Interleukin-6 Levels in Hepatocellular Carcinoma 10.5505:tjo.2018.1742
Intensive Care Medicine Experimental Elevated plasma glypicans are associated with organ failure in patients with infection Pubmed: 30618011
VALUE OF SERUM GLYPICAN 3 AS A PREDICTIVE TOOL FOR EARLY DIAGNOSIS OF HEPATOCELLULAR CARCINOMA (HCC)
ADVANCED RESEARCHES Search for Effective Serum Tumor Markers for Early Diagnosis of Hepatocellular Carcinoma Associated with Hepatitis C
Life Sci Selenium nanoparticles overcomes sorafenib resistance in thioacetamide induced hepatocellular carcinoma in rats by modulation of mTOR, NF-κB pathways and … Pubmed:35640776
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPA971Hu02 Recombinant Glypican 3 (GPC3) Positive Control; Immunogen; SDS-PAGE; WB.
APA971Hu02 Active Glypican 3 (GPC3) Cell culture; Activity Assays.
RPA971Hu01 Recombinant Glypican 3 (GPC3) Positive Control; Immunogen; SDS-PAGE; WB.
APA971Hu01 Active Glypican 3 (GPC3) Cell culture; Activity Assays.
PAA971Hu01 Polyclonal Antibody to Glypican 3 (GPC3) WB; ICC; IHC-P; IHC-F; ELISA.
MAA971Hu21 Monoclonal Antibody to Glypican 3 (GPC3) WB; IHC; ICC; IP.
MAA971Hu22 Monoclonal Antibody to Glypican 3 (GPC3) WB; IHC; ICC; IP.
MAA971Hu24 Monoclonal Antibody to Glypican 3 (GPC3) WB; IHC; ICC; IP.
MAA971Hu23 Monoclonal Antibody to Glypican 3 (GPC3) WB; IHC; ICC; IP.
MAA971Hu25 Monoclonal Antibody to Glypican 3 (GPC3) WB; IHC; ICC; IP.
FAA971Hu82 FITC-Linked Anti-Glypican 3 (GPC3) Monoclonal Antibody Flow cytometry.
SEA971Hu ELISA Kit for Glypican 3 (GPC3) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA971Hu Multiplex Assay Kit for Glypican 3 (GPC3) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.