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Active Tryptase (TPS)

TPSAB1; Tryptase Alpha/Beta 1; Tryptase Alpha II; Tryptase Beta I; Tryptase-I; Tryptase-II; Tryptase-III

ACTIVITY TEST

Tryptase is a serine protease with trypsin-like activity, which is sometimes also referred to as Mast Cell Protease 7. It is stored in the secretory granules of mouse mast cells. It exhibits anticoagulant activity due to its ability to degrade fibrinogen in the presence of the diverse array of protease inhibitors in plasma. The activity of recombinant human TPS is measured by its ability to cleave a fluorogenic peptide substrate Mca-Arg-Pro-Lys-Pro-Val-Glu-Nval-Trp-Arg-Lys(Dnp)-NH2 in the assay buffer 50 mM Tris, pH 8.5. The rhTPS is diluted to 200 ug/ml in 50 mM Tris, 150 mM NaCl, 10 mM CaCl2, 0.05% (w/v) Brij-35, pH 7.5, then activated with 0.1 ug/ml Thermolysin at 37 ℃ for 15min followed by adding 10 mM 1, 10 phenanthroline to stop activation. The activated rhTPS is diluted to 50 ug/mL in heparin incubation buffer of 100 µg/mL heparin, 50 mM MES, pH 5.5 and incubated at room temperature for 2 hours. Then the rhTPS was diluted to 12.5 ug/ml in assay buffer and load into a black well plate 50 µL and start the reaction by adding 50 µL of 20 µM substrate, with a substrate blank containing 50 µL assay buffer, 50 µL substrate, and no rhTPS. Then read at excitiation and emission wavelengths of 320 nm and 405 nm, respectively, in kinetic mode for 5 minutes. The specific activity of recombinant human TPS is > 25 pmol/min/µg.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Journal of Allergy and Clinical Immunology Glandular mast cells with distinct phenotype are highly elevated in chronic rhinosinusitis with nasal polyps PubMed: 22534535
J Pediatr. Improved liver function and relieved pruritus after 4-phenylbutyrate therapy in a patient with progressive familial intrahepatic cholestasis type 2 Pubmed:24530123
Mediators of Inflammation Mediators of Mast Cells in Bullous Pemphigoid and Dermatitis Herpetiformis Pubmed:25400334
PLOS ONE A Novel Model of IgE-Mediated Passive Pulmonary Anaphylaxis in Rats Pubmed:25541997
Neurogastroenterology & Motility Peritoneal mast cell degranulation and gastrointestinal recovery in patients undergoing colorectal surgery Pubmed:25677271
Mediators Inflamm Elevated Serum Tryptase and Endothelin in Patients with ST Segment Elevation Myocardial Infarction: Preliminary Report PubMed: 26089601
Int Immunopharmacol Tryptase and protease-activated receptor-2 stimulate scratching behavior in a murine model of ovalbumin-induced atopic-like dermatitis PubMed: 26049029
Mediators of Inflammation Altered expression of IFN-λ2 in allergic airway disorders and identification of its cell origins Journals: Mi
Mediators of Inflammation Altered Expression of IFN-2 in Allergic Airway Disorders and Identification of Its Cell Origins Pubmed:27057098
Analytica Chimica Acta An ultrasensitive electrochemical detection of tryptase using 3D macroporous reduced graphene oxide nanocomposites by one-pot electrochemical synthesis
Journal of Clinical Investigation Dengue virus-elicited tryptase induces endothelial permeability and shock Pubmed: 31265436
Frontiers in Physiology Mast Cells in Peritoneal Fluid From Women With Endometriosis and Their Possible Role in Modulating Sperm Function Pubmed: 31998139
Biomarkers of Allergic Asthma and their Association with Serum Parameters
Annals of Tropical Medicine & Public Health Effectiveness of biomarkers and serum parameters in determination allergic asthma and detection of its severity
Front Immunol Serum CD203c+ Extracellular Vesicle Serves as a Novel Diagnostic and Prognostic Biomarker for Succinylated Gelatin Induced Perioperative Hypersensitive … 34650557
Microbiol Spectr Mast Cell-Derived Proteases Induce Endothelial Permeability and Vascular Damage in Severe Fever with Thrombocytopenia Syndrome Pubmed:35612327
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
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