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ELISA Kit DIY Materials for Tryptase (TPS)
TPSAB1; Tryptase Alpha/Beta 1; Tryptase Alpha II; Tryptase Beta I; Tryptase-I; Tryptase-II; Tryptase-III
- Product No.KSB070Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range15.6-1,000pg/mL
- Applicable Sensitivity5.5pg/mL
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
- DownloadInstruction Manual
- UOM 96T*596T*10 96T*20 96T*50 96T*100
- FOB
US$ 1197
US$ 1995
US$ 3591
US$ 6983
US$ 11970
For more details, please contact local distributors!
SPECIFITY
The Abs in the kit have high sensitivity and excellent specificity for detection of Tryptase (TPS)). No significant cross-reactivity or interference between Tryptase (TPS) and analogues was observed.
USAGE
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
STORAGE
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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| Magazine | Citations |
| Journal of Allergy and Clinical Immunology | Glandular mast cells with distinct phenotype are highly elevated in chronic rhinosinusitis with nasal polyps PubMed: 22534535 |
| J Pediatr. | Improved liver function and relieved pruritus after 4-phenylbutyrate therapy in a patient with progressive familial intrahepatic cholestasis type 2 Pubmed:24530123 |
| Mediators of Inflammation | Mediators of Mast Cells in Bullous Pemphigoid and Dermatitis Herpetiformis Pubmed:25400334 |
| PLOS ONE | A Novel Model of IgE-Mediated Passive Pulmonary Anaphylaxis in Rats Pubmed:25541997 |
| Neurogastroenterology & Motility | Peritoneal mast cell degranulation and gastrointestinal recovery in patients undergoing colorectal surgery Pubmed:25677271 |
| Mediators Inflamm | Elevated Serum Tryptase and Endothelin in Patients with ST Segment Elevation Myocardial Infarction: Preliminary Report PubMed: 26089601 |
| Int Immunopharmacol | Tryptase and protease-activated receptor-2 stimulate scratching behavior in a murine model of ovalbumin-induced atopic-like dermatitis PubMed: 26049029 |
| Mediators of Inflammation | Altered expression of IFN-λ2 in allergic airway disorders and identification of its cell origins Journals: Mi |
| Mediators of Inflammation | Altered Expression of IFN-2 in Allergic Airway Disorders and Identification of Its Cell Origins Pubmed:27057098 |
| Analytica Chimica Acta | An ultrasensitive electrochemical detection of tryptase using 3D macroporous reduced graphene oxide nanocomposites by one-pot electrochemical synthesis |
| Journal of Clinical Investigation | Dengue virus-elicited tryptase induces endothelial permeability and shock Pubmed: 31265436 |
| Frontiers in Physiology | Mast Cells in Peritoneal Fluid From Women With Endometriosis and Their Possible Role in Modulating Sperm Function Pubmed: 31998139 |
| Biomarkers of Allergic Asthma and their Association with Serum Parameters | |
| Annals of Tropical Medicine & Public Health | Effectiveness of biomarkers and serum parameters in determination allergic asthma and detection of its severity |
| Front Immunol | Serum CD203c+ Extracellular Vesicle Serves as a Novel Diagnostic and Prognostic Biomarker for Succinylated Gelatin Induced Perioperative Hypersensitive … 34650557 |
| Microbiol Spectr | Mast Cell-Derived Proteases Induce Endothelial Permeability and Vascular Damage in Severe Fever with Thrombocytopenia Syndrome Pubmed:35612327 |
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| APB070Hu02 | Active Tryptase (TPS) | Cell culture; Activity Assays. |
| RPB070Hu01 | Recombinant Tryptase (TPS) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB070Hu02 | Recombinant Tryptase (TPS) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB070Hu01 | Polyclonal Antibody to Tryptase (TPS) | WB; IHC; ICC; IP. |
| LAB070Hu71 | Biotin-Linked Polyclonal Antibody to Tryptase (TPS) | WB; IHC; ICC. |
| MAB070Hu21 | Monoclonal Antibody to Tryptase (TPS) | WB; IHC; ICC; IP. |
| MAB070Hu24 | Monoclonal Antibody to Tryptase (TPS) | WB,IHC |
| MAB070Hu26 | Monoclonal Antibody to Tryptase (TPS) | WB,IHC |
| MAB070Hu22 | Monoclonal Antibody to Tryptase (TPS) | WB; IHC |
| MAB070Hu23 | Monoclonal Antibody to Tryptase (TPS) | WB; IHC. |
| LAB070Hu76 | Biotin-Linked Monoclonal Antibody to Tryptase (TPS) | WB; IHC; ICC. |
| FAB070Hu86 | FITC-Linked Anti-Tryptase (TPS) Monoclonal Antibody | FCM |
| WEB070Hu | Wide-range ELISA Kit for Tryptase (TPS) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEB070Hu | ELISA Kit for Tryptase (TPS) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCB070Hu | CLIA Kit for Tryptase (TPS) | Chemiluminescent immunoassay for Antigen Detection. |
| LMB070Hu | Multiplex Assay Kit for Tryptase (TPS) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
| PSB070Hu01 | Antibody Pair for Tryptase (TPS) | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| KSB070Hu01 | ELISA Kit DIY Materials for Tryptase (TPS) | Main materials for "Do It (ELISA Kit) Yourself". |