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Active Taxilin Alpha (TXLNa)

IL14; TXLN; HMW-BCGF; High Molecular Weight B-Cell Growth Factor; Interleukin 14

ACTIVITY TEST

Figure. The binding activity of TXLNa with PDIA3.
Taxilin Alpha (TXLNa) also known as interleukin-14 (IL-14) is a cytokine that controls the growth and proliferation of both normal and cancerous B cells. TXLNa induces B-cell proliferation, inhibits antibody secretion, and expands selected B-cell subgroups. This interleukin is produced mainly by T cells and certain malignant B cells. Besides, Protein Disulfide Isomerase A3 (PDIA3) has been identified as an interactor of TXLNa, thus a binding ELISA assay was conducted to detect the interaction of recombinant human TXLNa and recombinant human PDIA3. Briefly, TXLNa were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to PDIA3-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-TXLNa pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of TXLNa and PDIA3 was shown in Figure 1, and this effect was in a dose dependent manner.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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