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Figure. SDS-PAGE
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Active Cluster Of Differentiation 7 (CD7)
GP40; LEU9; TP41; Tp40; p41 Protein; T-cell Antigen CD7; T-cell Leukemia Antigen; T-cell surface antigen Leu-9
- Product No.APB784Hu61
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 5% Trehalose.
- Traits Freeze-dried powder
- Purity> 95%
- Isoelectric Point4.8
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
- FOB
US$ 180
US$ 450
US$ 900
US$ 2700
US$ 6750
For more details, please contact local distributors!
ACTIVITY TEST
Cluster Of Differentiation 7 (CD7) is a member of the immunoglobulin superfamily. This protein is found on thymocytes and mature T cells. It plays an essential role in T-cell interactions and also in T-cell/B-cell interaction during early lymphoid development. SECTM1 has been proposed as a ligand for CD7. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human CD7 and recombinant human SECTM1. Briefly, biotin-linked CD7 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to SECTM1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µl stop solution to the wells and read at 450 nm immediately. The binding activity of CD7 and SECTM1 was shown in Figure 1, and this effect was in a dose dependent manner.
USAGE
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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