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Active Histone Deacetylase 1 (HDAC1)

HDAC1; GON10; HD1; RPD3; RPD3L1

ACTIVITY TEST

Histone deacetylase 1 (HDAC1) is an enzyme which belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. HDAC1 also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Besides, Specificity Protein 1 (Sp1) has been identified as an interactor of HDAC1, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse HDAC1 and recombinant human Sp1. Briefly, HDAC1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to Sp1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-HDAC1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450 nm immediately. The binding activity of of recombinant mouse HDAC1 and recombinant human Sp1 was shown in Figure 1, and this effect was in a dose dependent manner.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Journal of Integrative Medicine Saponins isolated from roots of Chlorophytum borivilianum reduce acute and chronic inflammation and histone deacetylase Pubmed:25609369
Tzu Chi Medical Journal Effects of tetrandrine and caffeine on cell viability and expression of mammalian target of rapamycin, phosphatase and tensin homolog, histone deacetylase 1, and histone acetyltransferase in glioma cells Science: Article
Restor Neurol Neurosci Uridine protects against hypoxic-ischemic brain injury by reducing histone deacetylase activity in neonatal rats PubMed: 26410212
Pharmacol Biochem Behav Lacosamide reduces HDAC levels in the brain and improves memory: Potential for treatment of Alzheimer's disease PubMed: 25931268
网页打不开 Effects of caffeine on cell viability and activity of histone deacetylase 1 and histone acetyltransferase in glioma cells S1016319016300295
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
APC206Mu01 Active Histone Deacetylase 1 (HDAC1) Cell culture; Activity Assays.
RPC206Mu01 Recombinant Histone Deacetylase 1 (HDAC1) Positive Control; Immunogen; SDS-PAGE; WB.
RPC206Mu02 Recombinant Histone Deacetylase 1 (HDAC1) Positive Control; Immunogen; SDS-PAGE; WB.
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SEC206Mu ELISA Kit for Histone Deacetylase 1 (HDAC1) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC206Mu Multiplex Assay Kit for Histone Deacetylase 1 (HDAC1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.