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Active Carbonic Anhydrase VII (CA7)

CA-VII; CAVII; Carbonate dehydratase VII

ACTIVITY TEST

Carbonic anhydrase catalyzes the reversible reaction of CO2  H2O = HCO3-  H , which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption. Carbonic Anhydrase VII encoded by the CA7 gene is a cytosolic protein predominantly expressed in the salivary gland. CA7 may have additional tissue distributions and functions. For example, studies with CA inhibitors provide evidence that human CA7 is the CA isozyme responsible for the anticonvulsant/antiepileptic activity of sulfonamides and sulfamates. In fact, CA7 has been shown in a rat model to act as a developmental switch in GABAergic signaling in neurons. The activity of recombinant mouse CA7 was measured by its ability to hydrolyze 4-Nitrophenyl acetate (4-NPA) to 4-Nitrophenol. The reaction was performed in 12.5 mM Tris, 75 mM NaCl, pH 7.5 (assay buffer), initiated by addition 50 μL of various concentrations of CA7 (diluted by assay buffer) to 50 µL of 2 mM substrate 4-NPA (100 mM stock in Acetone, diluted by assay buffer). Incubated at 37℃ for 5min, then read at a wavelength of 400 nm.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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INCREMENT SERVICES

Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPD074Mu01 Recombinant Carbonic Anhydrase VII (CA7) Positive Control; Immunogen; SDS-PAGE; WB.
APD074Mu01 Active Carbonic Anhydrase VII (CA7) Cell culture; Activity Assays.
PAD074Mu01 Polyclonal Antibody to Carbonic Anhydrase VII (CA7) WB; IHC; ICC; IP.
LAD074Mu71 Biotin-Linked Polyclonal Antibody to Carbonic Anhydrase VII (CA7) WB; IHC; ICC.