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Active Cold Inducible RNA Binding Protein (CIRBP)

CIRP; A18HNRNP; Glycine-Rich RNA Binding Protein

ACTIVITY TEST

CIRBP (Cold-inducible RNA-binding protein) is considered to play a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival and act as a translational activator. Besides, ATXN1 (Ataxin-1) has been proven as an interactor of CIRBP. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human CIRBP and recombinant human ATXN1. Briefly, CIRBP were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to ATXN1-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-CIRBP mAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of CIRBP and ATXN1 was shown in Figure 1, and this effect was in a dose dependent manner.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
J Cell Mol Med Normothermic machine perfusion attenuates hepatic ischaemia‐reperfusion injury by inhibiting CIRP‐mediated oxidative stress and mitochondrial fission 34786826
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