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High Sensitive ELISA Kit for Inhibin Beta E (INHbE)
INH-bE; Activin beta-E chain
- Product No.HEA048Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range1.56-100pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.58pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
- FOB
US$ 499
US$ 713
US$ 3209
US$ 6061
US$ 49910
For more details, please contact local distributors!
Specificity
This assay has high sensitivity and excellent specificity for detection of High Sensitive Inhibin Beta E (INHbE).
No significant cross-reactivity or interference between High Sensitive Inhibin Beta E (INHbE) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant High Sensitive Inhibin Beta E (INHbE) and the recovery rates were calculated by comparing the measured value to the expected amount of High Sensitive Inhibin Beta E (INHbE) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 86-103 | 98 |
| EDTA plasma(n=5) | 78-95 | 89 |
| heparin plasma(n=5) | 89-96 | 92 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Inhibin Beta E (INHbE) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Inhibin Beta E (INHbE) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of High Sensitive Inhibin Beta E (INHbE) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 89-96% | 94-101% | 93-103% | 78-98% |
| EDTA plasma(n=5) | 82-92% | 92-101% | 80-101% | 78-102% |
| heparin plasma(n=5) | 93-101% | 87-101% | 82-104% | 98-105% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
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| Magazine | Citations |
| Mitochondrion | GDF15 is a novel biomarker to evaluate efficacy of pyruvate therapy for mitochondrial diseases Pubmed:25446397 |
| PLoS One | Inhibin βE (INHBE) is a possible insulin resistance-associated hepatokine identified by comprehensive gene expression analysis in human liver biopsy … Pubmed:29596463 |
| Cell Reports | Activin E Controls Energy Homeostasis in Both Brown and White Adipose Tissues as a Hepatokine Pubmed: 30380411 |
| International Journal of Pharmacy and Biological Sciences | Anti-Obesity Efficacy of Selected Plants Against Progesterone Induced Obesity |
| Catalog No. | Related products for research use of Mus musculus (Mouse) Organism species | Applications (RESEARCH USE ONLY!) |
| RPA048Mu01 | Recombinant Inhibin Beta E (INHbE) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA048Mu02 | Recombinant Inhibin Beta E (INHbE) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA048Mu01 | Polyclonal Antibody to Inhibin Beta E (INHbE) | WB; IHC |
| PAA048Mu02 | Polyclonal Antibody to Inhibin Beta E (INHbE) | WB; IHC; ICC; IP. |
| SEA048Mu | ELISA Kit for Inhibin Beta E (INHbE) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| HEA048Mu | High Sensitive ELISA Kit for Inhibin Beta E (INHbE) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CEA048Mu | ELISA Kit for Inhibin Beta E (INHbE) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA048Mu | Multiplex Assay Kit for Inhibin Beta E (INHbE) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |