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ELISA Kit DIY Materials for Complement Component 5a (C5a)
- Product No.KSA388Mu01
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range1.56-100ng/mL
- Applicable Sensitivity0.57ng/mL
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
- Downloadn/a
- UOM 96T*596T*10 96T*20 96T*50 96T*100
- FOB
US$ 1037
US$ 1728
US$ 3110
US$ 6048
US$ 10368
For more details, please contact local distributors!
SPECIFITY
The Abs in the kit have high sensitivity and excellent specificity for detection of Complement Component 5a (C5a). No significant cross-reactivity or interference between Complement Component 5a (C5a) and analogues was observed.
USAGE
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
STORAGE
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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| Magazine | Citations |
| Molecular Immunology | Cathepsin D is released after severe tissue trauma in vivo and is capable of generating C5a in vitro ScienceDirect: S0161589011008297 |
| Journal of Reproductive Immunology | Elevated complement factor C5a in maternal and umbilical cord plasma in preeclampsia PubMed: 23415845 |
| The Journal of Clinical Investigation | Complement component 5 contributes to poor disease outcome in humans and mice with pneumococcal meningitis PubMed: PMC3195471 |
| Journal of Antimicrobial Chemotherapy | Efficacy of a synthetic antimicrobial peptidomimetic versus vancomycin in a Staphylococcus epidermidis device-related murine peritonitis model Pubmed: 23645587 |
| Journal of Orthopaedic Research | Conversion from external fixator to intramedullary nail causes a second hit and impairs fracture healing in a severe trauma model Pubmed: 23070742 |
| SpringerPlus | Therapeutic effects of sesame oil on monosodium urate crystal-induced acute inflammatory response in rats Pubmed: 24353977 |
| American Journal fo Reproductive Immunology | Evaluation of the Markers of Inflammation in the Umbilical Cord Blood of Newborns of Mothers with Thrombophilia Pubmed:25209155 |
| Gynecol Obstet Invest | Complement Split Products C3a/C5a and Receptors: Are They Regulated by Circulating Angiotensin II Type 1 Receptor Autoantibody in Severe Preeclampsia? PubMed: 26485247 |
| Journal of Neuroimmunology | Acute and prolonged complement activation in the central nervous system during herpes simplex encephalitis Pubmed:27235358 |
| International Immunopharmacology | Consistency and pathophysiological characterization of a rat polymicrobial sepsis model via the improved cecal ligation and puncture surgery Pubmed:26802602 |
| Digital Repository | Dosagem de frações ativadas do sistema complemento em empiema induzido em ratos 10183 |
| Environmental Pollution | Cadmium-induced immune abnormality is a key pathogenic event in human and rat models of preeclampsia pubmed:27511439 |
| Cancer Letters | Complement C5a/C5aR pathway potentiates the pathogenesis Q5 of gastric cancer by down-regulating p21 expression pubmed:29031586 |
| Acta Paediatrica | Serum complement factor 5a levels are associated with nonalcoholic fatty liver disease in obese children DOI: 10.1111/apa.14106 |
| Scientific Reports | Splenectomy modulates early immuno-inflammatory responses to trauma-hemorrhage and protects mice against secondary sepsis Pubmed: 30291296 |
| Nutrients | The Beneficial Effects of Lactobacillus plantarum PS128 on High-Intensity, Exercise-Induced Oxidative Stress, Inflammation, and Performance in Triathletes Pubmed: 30736479 |
| Journal of NeuroVirology | Intrathecal complement activation by the classical pathway in tick-borne encephalitis Pubmed: 30850976 |
| PLoS One | Sepsis causes right ventricular myocardial inflammation independent of pulmonary hypertension in a porcine sepsis model Pubmed: 31247004 |
| Frontiers in Immunology | C5a/C5aR Pathway is Critical for the Pathogenesis of Psoriasis Pubmed: 31447855 |
| Phytomedicine | Novel evidence that an alternative complement cascade pathway is involved in optimal mobilization of hematopoietic stem/progenitor cells in Nlrp3 … |
| Aging | C5aR deficiency attenuates the breast cancer development via the p38/p21 axis Pubmed: 32669478 |
| STEM CELL REVIEWS AND REPORTS | A Novel Evidence That Mannan Binding Lectin (MBL) Pathway of Complement Cascade Activation is Involved in Homing and Engraftment of Hematopoietic Stem … Pubmed: 32406006 |
| Modulation of C5a-C5aR interactions against murine mammary cancer cell line | |
| FASEB J | C‐reactive protein inhibits C3a/C3aR‐dependent podocyte autophagy in favor of diabetic kidney disease Pubmed:35503088 |
| Catalog No. | Related products for research use of Mus musculus (Mouse) Organism species | Applications (RESEARCH USE ONLY!) |
| RPA388Mu01 | Recombinant Complement Component 5a (C5a) | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPA388Mu62 | Eukaryotic Complement Component 5a (C5a) | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA388Mu61 | Active Complement Component 5a (C5a) | Cell culture; Activity Assays. |
| APA388Mu62 | Active Complement Component 5a (C5a) | Cell culture; Activity Assays. |
| EPA388Mu61 | Eukaryotic Complement Component 5a (C5a) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA388Mu01 | Polyclonal Antibody to Complement Component 5a (C5a) | WB; IHC; ICC; IP. |
| LAA388Mu71 | Biotin-Linked Polyclonal Antibody to Complement Component 5a (C5a) | WB; IHC; ICC. |
| MAA388Mu21 | Monoclonal Antibody to Complement Component 5a (C5a) | WB; IHC; ICC; IP. |
| LAA388Mu72 | Biotin-Linked Monoclonal Antibody to Complement Component 5a (C5a) | WB; IHC; ICC. |
| SEA388Mu | ELISA Kit for Complement Component 5a (C5a) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEA388Mu | Mini Samples ELISA Kit for Complement Component 5a (C5a) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| HEA388Mu | High Sensitive ELISA Kit for Complement Component 5a (C5a) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA388Mu | CLIA Kit for Complement Component 5a (C5a) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA388Mu | Multiplex Assay Kit for Complement Component 5a (C5a) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
| PSA388Mu01 | Antibody Pair for Complement Component 5a (C5a) | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| KSA388Mu01 | ELISA Kit DIY Materials for Complement Component 5a (C5a) | Main materials for "Do It (ELISA Kit) Yourself". |