Mini Samples ELISA Kit for Complement Component 5a (C5a)

This assay has high sensitivity and excellent specificity for detection of Mini Samples Complement Component 5a (C5a).
No significant cross-reactivity or interference between Mini Samples Complement Component 5a (C5a) and analogues was observed.

Matrices listed below were spiked with certain level of recombinant Mini Samples Complement Component 5a (C5a) and the recovery rates were calculated by comparing the measured value to the expected amount of Mini Samples Complement Component 5a (C5a) in samples.

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Complement Component 5a (C5a) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Complement Component 5a (C5a) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mini Samples Complement Component 5a (C5a) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

1. Prepare all reagents, samples and standards;
2. Add 25µL standard or sample to each well. Incubate 1 hour at 37°C;
3. Aspirate and add 25µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 25µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 25µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 20µL Stop Solution. Read at 450nm immediately.

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