ELISA Kit DIY Materials for Oxidized Low Density Lipoprotein (OxLDL)

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  • Product No.KSA527Mu11
  • Organism SpeciesMus musculus (Mouse) Same name, Different species.
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma and other biological fluids.
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range2.47-200ng/mL
  • Applicable Sensitivity0.89ng/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1296  US$ 2160  US$ 3888  US$ 7560  US$ 12960 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Oxidized Low Density Lipoprotein (OxLDL). No significant cross-reactivity or interference between Oxidized Low Density Lipoprotein (OxLDL) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Journal of Ethnopharmacology Total panax notoginsenosides prevent atherosclerosis in apolipoprotein E-knockout mice: Role of downregulation of CD40 and MMP-9 expression ScienceDirect: S0378874109005091
Autonomic Neuroscience Chemical sympathectomy induces arterial accumulation of native and oxidized LDL in hypercholesterolemic rats ScienceDirect: S1566070211004152
Cell Biochemistry and Biophysics Plasma Paraoxonase-1, Oxidized Low-Density Lipoprotein and Lipid Peroxidation Levels in Gout Patients SpringerLink: u466114p03434m6t
Cardiovascular Research Endothelial-specific Nox2 overexpression increases vascular superoxide and macrophage recruitment in ApoE-/- mice PubMed: PMC3307381
Atherosclerosis 10-Dehydrogingerdione raises HDL-cholesterol through a CETP inhibition and wards off oxidation and inflammation in dyslipidemic rabbits. Pubmed: 24267247
PloS one Atheroprotective effect of oleoylethanolamide (OEA) targeting oxidized LDL. NCBI: PMC3896367
Atherosclerosis.? Insulin-like growth factor I reduces lipid oxidation and foam cell formation via downregulation of 12/15-lipoxygenase Pubmed:25549319
J Surg Arts THE FUNCTION OF SIALIC ACID AS A RADICAL SCAVENGER IN EXPERIMENTAL HYPOTHYROIDISM WITH AND WITHOUT HYPERLIPIDEMIA. Cerrahisanatlardergisi:Source
PLoS One. Atheroprotective effect of oleoylethanolamide (OEA) targeting oxidized LDL Pubmed:24465540
Saudi Med J Increased systemic low-grade inflammation in high altitude native rats mediated by adrenergic receptors Pubmed:24888651
Vascular Medicine Anti‐Inflammatory Immune Skewing Is Atheroprotective: Apoe−/−FcγRIIb−/− Mice Develop Fibrous Carotid Plaques Pubmed:25516435
Journal of Functional Foods Oral administration of the β-glucan produced by Aureobasidium pullulans ameliorates development of atherosclerosis in apolipoprotein E deficient mice Science: Article
Acta Pharmacologica Sinica Sirt1 is involved in decreased bone formation in aged apolipoprotein E-deficient mice PubMed: 26592520
Cancer Science Suppressive effects of the NADPH oxidase inhibitor apocynin on intestinal tumorigenesis in obese KK‐Ay and Apc mutant Min mice PubMed: 26310859
BMJ Open Sport Exerc Med Athletes with higher VO2max present reduced oxLDL after a marathon race Content: 1
Biochim Biophys Acta PPARβ/δ ameliorates fructose-induced insulin resistance in adipocytes by preventing Nrf2 activation PubMed: 25728706
PLoS One Ezetimibe Attenuates Atherosclerosis Associated with Lipid Reduction and Inflammation Inhibition PubMed: 26555472
PLoS One Pulsatile Hyperglycaemia Induces Vascular Oxidative Stress and GLUT 1 Expression More Potently than Sustained Hyperglycaemia in Rats on High Fat Diet Pubmed:26790104
Diabetology & Metabolic Syndrome Oxidative DNA damage and oxidized low density lipoprotein in Type II diabetes mellitus among patients with Helicobacter pylori infection pmc:PMC4855822
J Am Heart Assoc. Transcriptional Profiling of Foam Cells Reveals Induction of Cavia (Guinea pig )anylate‐Binding Proteins Following Western Diet Acceleration of Atherosclerosis in the Absence of Global Changes in Inflammation pmc:PMC4859273
Journal of Applied Pharmaceutical Science Organic Arsenical Exposure Stimulates Atherosclerosis through Oxidative Stress Increase and Adhesion Molecule Expression admin:php
Journal of Nutritional Biochemistry Dietary rose hip exerts anti-atherosclerotic effects and increases nitric oxide-mediated dilation in ApoE-null mice S0955286316301887
Journal of Basic and Clinical Physiology and Pharmacology Effects of altered thyroid states on oxidative stress parameters in rats pubmed:27824612
Journal of Functional Foods Atherosclerosis-related inflammation and oxidative stress are improved by rice bran enzymatic extract S1756464616302511
Microvascular Research Effects of insulin analogs as an add-on to metformin on cutaneous microcirculation in type 2 diabetic patients pubmed:28954218
Nutrients Anti-Atherogenic Activity of Polyphenol-Rich Extract from Bee Pollen pubmed:29258230
Biochemical and Biophysical Research Communications Oxidized low-density lipoprotein promotes osteoclast differentiation from CD68 positive mononuclear cells by regulating HMGB1 release pubmed:29146189
Journal of Surgical Research THE FUNCTION OF SIALIC ACID AS A RADICAL SCAVENGER IN EXPERIMENTAL HYPOTHYROIDISM WITH AND WITHOUT HYPERLIPIDEMIA 10.14717/jsurgarts.2014.163
biorxiv Significant metabolic improvement by a water extract of olives: animal and human evidence 10.1101/151860
Journal of nutritional biochemistry Dietary rose hip exerts antiatherosclerotic effects and increases nitric oxide-mediated dilation in ApoE-null mice. pubmed:28399420
Nutrients Yirui Capsules Alleviate Atherosclerosis by Improving the Lipid Profile and Reducing Inflammation in Apolipoprotein E-Deficient Mice Pubmed:29382111
Nutrition & Metabolism Cold-pressed minke whale oil reduces circulating LDL/VLDL-cholesterol, lipid oxidation and atherogenesis in apolipoprotein E-deficient mice fed a Western … Pubmed:29755576
Journal of Functional Foods Saponins of sea cucumber attenuate atherosclerosis in ApoE−/− mice via lipid-lowering and anti-inflammatory properties 10.1016:j.jff.2018.07.046
Journal of Agricultural and Food Chemistry Neuroprotective effect of hydroxytyrosol in experimental diabetic retinopathy: relationship with cardiovascular biomarkers Pubmed:29278909
Molecules Protective Effect of Polyphenol-Rich Extract from Bee Pollen in a High-Fat Diet Pubmed:29614743
Lipids in Health and Disease Lipingshu capsule improves atherosclerosis associated with lipid regulation and inflammation inhibition in apolipoprotein E–deficient mice Pubmed:30064511
Diabetes Metabolism Research and Reviews  Diabetic dyslipidaemia is associated with alterations in eNOS, caveolin‐1, and endothelial dysfunction in streptozotocin treated rats Pubmed:29471582
Diabetes & Metabolic Syndrome: Clinical Research & Reviews Beyond LDL-c: The importance of serum oxidized LDL in predicting risk for type 2 diabetes in the middle-aged Asian Indians Doi: 10.1016/j.dsx.2018.08.036
中国药理学通报 三七总皂苷对动脉粥样硬化小鼠的治疗作用
Biomedicine & Pharmacotherapy Effects of lycopene on metabolism of glycolipid in type 2 diabetic rats Pubmed: 30551463
Journal of Ethnopharmacology Anti-diabetic properties of different fractions of Korean red ginseng
Chinese Pharmacological Bulletin中国药理学通报 地昔帕明对兔动脉斑块细胞凋亡及内质网应激的影响
Document Version
MEDICINE Screening of schizophrenia associated miRNAs and the regulation of miR-320a-3p on integrin β1 Pubmed: 30813134
Pharmacological Research Ethanol extracts of Danlou tablet attenuate atherosclerosis via inhibiting inflammation and promoting lipid effluent Pubmed: 31181336
Oxidative Medicine and Cellular Longevity Combined Exercise Training Performed by Elderly Women Reduces Redox Indexes and Proinflammatory Cytokines Related to Atherogenesis
Nature Communications ER-residential Nogo-B accelerates NAFLD-associated HCC mediated by metabolic reprogramming of oxLDL lipophagy Pubmed: 31358770
Clinical and Experimental Medicine Senescence-associated miR-34a and miR-126 in middle-aged Indians with type 2 diabetes Pubmed: 31732824
Steroids Serum 25 (OH) D concentration and its association with inflammation and oxidative stress in the middle-aged Indian healthy and diabetic subjects Pubmed: 31672627
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY Zygophyllum album saponins prevent atherogenic effect induced by deltamethrin via attenuating arterial accumulation of native and oxidized LDL in rats Pubmed: 32105945
JOURNAL OF CARDIOVASCULAR PHARMACOLOGY Ginsenoside Rb1 Alleviates Oxidative Low-Density Lipoprotein–Induced Vascular Endothelium Senescence via the SIRT1/Beclin-1/Autophagy Axis Pubmed: 31658172
Journal of Diabetes Research Middle-Aged Indians with Type 2 Diabetes Are at Higher Risk of Biological Ageing with Special Reference to Serum CDKN2A Pubmed: 32280716
Acta Biochim Pol Dietary Lactobacillus plantarum LS/07 and inulin in the management of chronic disease risk factors Pubmed: 33108141
Ecotoxicol Environ Saf Permethrin induced arterial retention of native and oxidized LDL in rats by promoting inflammation, oxidative stress and affecting LDL receptors, and collagen … Pubmed: 32911180
Cell Death & Disease Oxidized-LDL inhibits testosterone biosynthesis by affecting mitochondrial function and the p38 MAPK/COX-2 signaling pathway in Leydig cells Pubmed: 32796811
Nutrients Ginseng-Induced Changes to Blood Vessel Dilation and the Metabolome of Rats Pubmed: 32727012
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES The Fungal Iron Chelator Desferricoprogen Inhibits Atherosclerotic Plaque Formation Pubmed: 32635347
Oxidative Medicine and Cellular Longevity Improvement of Endothelial Dysfunction of Berberine in Atherosclerotic Mice and Mechanism Exploring through TMT-Based Proteomics Pubmed: 32566106
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE The microvesicle/CD36 complex triggers a prothrombotic phenotype in patients with non‐valvular atrial fibrillation Pubmed: 32510852
Antioxidants Nicotinamide Prevents Apolipoprotein B-Containing Lipoprotein Oxidation, Inflammation and Atherosclerosis in Apolipoprotein E-Deficient Mice 33233455
MOLECULAR NUTRITION & FOOD RESEARCH Digestive n©\6 Lipid Oxidation, a Key Trigger of Vascular Dysfunction and Atherosclerosis in the Western Diet: Protective Effects of Apple Polyphenols 33450108
Scientific reports Genetic deletion of Abcc6 disturbs cholesterol homeostasis in mice 33483533
Scientific reports Antibodies against apoB100 peptide 210 inhibit atherosclerosis in apoE-/-mice 33907226
Journal of Food and Nutrition Research Effect of Hibiscus sabdariffa Tea Extracts Intake on the Atherosclerosis Biomarkers in Adults
Journal of Food and Nutrition Research Regulation of CD36 Gene Expression by Hibiscus Sabdariffa Tea Extracts to Affect the Atherosclerosis Biomarkers in Saudi Women
Eur J Nutr The effects of long-term moderate exercise and Western-type diet on oxidative/nitrosative stress, serum lipids and cytokines in female Sprague Dawley rats 34319428
Pediatr Gastroenterol Hepatol Nutr The Relationship between Daily Fructose Consumption and Oxidized Low-Density Lipoprotein and Low-Density Lipoprotein Particle Size in Children with … 34557400
J Mol Cell Cardiol ILF3 is responsible for hyperlipidemia-induced arteriosclerotic calcification by mediating BMP2 and STAT1 transcription 34343541
J Basic Clin Physiol Pharmacol Anti-atherosclerotic activity of aqueous extract of Ipomoea batatas (L.) leaves in high-fat diet-induced atherosclerosis model rats Pubmed:34986543
Oxid Med Cell Longev DNA Methyltransferase 3b Accelerates the Process of Atherosclerosis Pubmed:35422896
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KSA527Mu11 ELISA Kit DIY Materials for Oxidized Low Density Lipoprotein (OxLDL) Main materials for "Do It (ELISA Kit) Yourself".