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Wide-range ELISA Kit for Oxidized Low Density Lipoprotein (OxLDL)
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- Product No.WEA527Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Sample Typeserum, plasma and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range3.12-200ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 1.34ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
- FOB
US$ 606
US$ 865
US$ 3893
US$ 7353
US$ 60550
For more details, please contact local distributors!
Specificity
This assay has high sensitivity and excellent specificity for detection of Wide-range Oxidized Low Density Lipoprotein (OxLDL).
No significant cross-reactivity or interference between Wide-range Oxidized Low Density Lipoprotein (OxLDL) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Wide-range Oxidized Low Density Lipoprotein (OxLDL) and the recovery rates were calculated by comparing the measured value to the expected amount of Wide-range Oxidized Low Density Lipoprotein (OxLDL) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 79-90 | 85 |
| EDTA plasma(n=5) | 91-102 | 96 |
| heparin plasma(n=5) | 92-105 | 98 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wide-range Oxidized Low Density Lipoprotein (OxLDL) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wide-range Oxidized Low Density Lipoprotein (OxLDL) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Wide-range Oxidized Low Density Lipoprotein (OxLDL) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 98-105% | 88-102% | 99-105% | 78-95% |
| EDTA plasma(n=5) | 79-93% | 87-98% | 97-105% | 81-101% |
| heparin plasma(n=5) | 88-97% | 80-94% | 88-102% | 96-105% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
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| Magazine | Citations |
| Journal of Ethnopharmacology | Total panax notoginsenosides prevent atherosclerosis in apolipoprotein E-knockout mice: Role of downregulation of CD40 and MMP-9 expression ScienceDirect: S0378874109005091 |
| Autonomic Neuroscience | Chemical sympathectomy induces arterial accumulation of native and oxidized LDL in hypercholesterolemic rats ScienceDirect: S1566070211004152 |
| Cell Biochemistry and Biophysics | Plasma Paraoxonase-1, Oxidized Low-Density Lipoprotein and Lipid Peroxidation Levels in Gout Patients SpringerLink: u466114p03434m6t |
| Cardiovascular Research | Endothelial-specific Nox2 overexpression increases vascular superoxide and macrophage recruitment in ApoE-/- mice PubMed: PMC3307381 |
| Atherosclerosis | 10-Dehydrogingerdione raises HDL-cholesterol through a CETP inhibition and wards off oxidation and inflammation in dyslipidemic rabbits. Pubmed: 24267247 |
| PloS one | Atheroprotective effect of oleoylethanolamide (OEA) targeting oxidized LDL. NCBI: PMC3896367 |
| Atherosclerosis.? | Insulin-like growth factor I reduces lipid oxidation and foam cell formation via downregulation of 12/15-lipoxygenase Pubmed:25549319 |
| J Surg Arts | THE FUNCTION OF SIALIC ACID AS A RADICAL SCAVENGER IN EXPERIMENTAL HYPOTHYROIDISM WITH AND WITHOUT HYPERLIPIDEMIA. Cerrahisanatlardergisi:Source |
| PLoS One. | Atheroprotective effect of oleoylethanolamide (OEA) targeting oxidized LDL Pubmed:24465540 |
| Saudi Med J | Increased systemic low-grade inflammation in high altitude native rats mediated by adrenergic receptors Pubmed:24888651 |
| Vascular Medicine | Anti‐Inflammatory Immune Skewing Is Atheroprotective: Apoe−/−FcγRIIb−/− Mice Develop Fibrous Carotid Plaques Pubmed:25516435 |
| Journal of Functional Foods | Oral administration of the β-glucan produced by Aureobasidium pullulans ameliorates development of atherosclerosis in apolipoprotein E deficient mice Science: Article |
| Acta Pharmacologica Sinica | Sirt1 is involved in decreased bone formation in aged apolipoprotein E-deficient mice PubMed: 26592520 |
| Cancer Science | Suppressive effects of the NADPH oxidase inhibitor apocynin on intestinal tumorigenesis in obese KK‐Ay and Apc mutant Min mice PubMed: 26310859 |
| BMJ Open Sport Exerc Med | Athletes with higher VO2max present reduced oxLDL after a marathon race Content: 1 |
| Biochim Biophys Acta | PPARβ/δ ameliorates fructose-induced insulin resistance in adipocytes by preventing Nrf2 activation PubMed: 25728706 |
| PLoS One | Ezetimibe Attenuates Atherosclerosis Associated with Lipid Reduction and Inflammation Inhibition PubMed: 26555472 |
| PLoS One | Pulsatile Hyperglycaemia Induces Vascular Oxidative Stress and GLUT 1 Expression More Potently than Sustained Hyperglycaemia in Rats on High Fat Diet Pubmed:26790104 |
| Diabetology & Metabolic Syndrome | Oxidative DNA damage and oxidized low density lipoprotein in Type II diabetes mellitus among patients with Helicobacter pylori infection pmc:PMC4855822 |
| J Am Heart Assoc. | Transcriptional Profiling of Foam Cells Reveals Induction of Cavia (Guinea pig )anylate‐Binding Proteins Following Western Diet Acceleration of Atherosclerosis in the Absence of Global Changes in Inflammation pmc:PMC4859273 |
| Journal of Applied Pharmaceutical Science | Organic Arsenical Exposure Stimulates Atherosclerosis through Oxidative Stress Increase and Adhesion Molecule Expression admin:php |
| Journal of Nutritional Biochemistry | Dietary rose hip exerts anti-atherosclerotic effects and increases nitric oxide-mediated dilation in ApoE-null mice S0955286316301887 |
| Journal of Basic and Clinical Physiology and Pharmacology | Effects of altered thyroid states on oxidative stress parameters in rats pubmed:27824612 |
| Journal of Functional Foods | Atherosclerosis-related inflammation and oxidative stress are improved by rice bran enzymatic extract S1756464616302511 |
| Microvascular Research | Effects of insulin analogs as an add-on to metformin on cutaneous microcirculation in type 2 diabetic patients pubmed:28954218 |
| Nutrients | Anti-Atherogenic Activity of Polyphenol-Rich Extract from Bee Pollen pubmed:29258230 |
| Biochemical and Biophysical Research Communications | Oxidized low-density lipoprotein promotes osteoclast differentiation from CD68 positive mononuclear cells by regulating HMGB1 release pubmed:29146189 |
| Journal of Surgical Research | THE FUNCTION OF SIALIC ACID AS A RADICAL SCAVENGER IN EXPERIMENTAL HYPOTHYROIDISM WITH AND WITHOUT HYPERLIPIDEMIA 10.14717/jsurgarts.2014.163 |
| biorxiv | Significant metabolic improvement by a water extract of olives: animal and human evidence 10.1101/151860 |
| Journal of nutritional biochemistry | Dietary rose hip exerts antiatherosclerotic effects and increases nitric oxide-mediated dilation in ApoE-null mice. pubmed:28399420 |
| Nutrients | Yirui Capsules Alleviate Atherosclerosis by Improving the Lipid Profile and Reducing Inflammation in Apolipoprotein E-Deficient Mice Pubmed:29382111 |
| Nutrition & Metabolism | Cold-pressed minke whale oil reduces circulating LDL/VLDL-cholesterol, lipid oxidation and atherogenesis in apolipoprotein E-deficient mice fed a Western … Pubmed:29755576 |
| Journal of Functional Foods | Saponins of sea cucumber attenuate atherosclerosis in ApoE−/− mice via lipid-lowering and anti-inflammatory properties 10.1016:j.jff.2018.07.046 |
| Journal of Agricultural and Food Chemistry | Neuroprotective effect of hydroxytyrosol in experimental diabetic retinopathy: relationship with cardiovascular biomarkers Pubmed:29278909 |
| Molecules | Protective Effect of Polyphenol-Rich Extract from Bee Pollen in a High-Fat Diet Pubmed:29614743 |
| Lipids in Health and Disease | Lipingshu capsule improves atherosclerosis associated with lipid regulation and inflammation inhibition in apolipoprotein E–deficient mice Pubmed:30064511 |
| Diabetes Metabolism Research and Reviews | Diabetic dyslipidaemia is associated with alterations in eNOS, caveolin‐1, and endothelial dysfunction in streptozotocin treated rats Pubmed:29471582 |
| Diabetes & Metabolic Syndrome: Clinical Research & Reviews | Beyond LDL-c: The importance of serum oxidized LDL in predicting risk for type 2 diabetes in the middle-aged Asian Indians Doi: 10.1016/j.dsx.2018.08.036 |
| 中国药理学通报 | 三七总皂苷对动脉粥样硬化小鼠的治疗作用 |
| Biomedicine & Pharmacotherapy | Effects of lycopene on metabolism of glycolipid in type 2 diabetic rats Pubmed: 30551463 |
| Journal of Ethnopharmacology | Anti-diabetic properties of different fractions of Korean red ginseng |
| Chinese Pharmacological Bulletin中国药理学通报 | 地昔帕明对兔动脉斑块细胞凋亡及内质网应激的影响 |
| Document Version | |
| MEDICINE | Screening of schizophrenia associated miRNAs and the regulation of miR-320a-3p on integrin β1 Pubmed: 30813134 |
| Pharmacological Research | Ethanol extracts of Danlou tablet attenuate atherosclerosis via inhibiting inflammation and promoting lipid effluent Pubmed: 31181336 |
| Oxidative Medicine and Cellular Longevity | Combined Exercise Training Performed by Elderly Women Reduces Redox Indexes and Proinflammatory Cytokines Related to Atherogenesis |
| Nature Communications | ER-residential Nogo-B accelerates NAFLD-associated HCC mediated by metabolic reprogramming of oxLDL lipophagy Pubmed: 31358770 |
| Clinical and Experimental Medicine | Senescence-associated miR-34a and miR-126 in middle-aged Indians with type 2 diabetes Pubmed: 31732824 |
| Steroids | Serum 25 (OH) D concentration and its association with inflammation and oxidative stress in the middle-aged Indian healthy and diabetic subjects Pubmed: 31672627 |
| ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY | Zygophyllum album saponins prevent atherogenic effect induced by deltamethrin via attenuating arterial accumulation of native and oxidized LDL in rats Pubmed: 32105945 |
| JOURNAL OF CARDIOVASCULAR PHARMACOLOGY | Ginsenoside Rb1 Alleviates Oxidative Low-Density Lipoprotein–Induced Vascular Endothelium Senescence via the SIRT1/Beclin-1/Autophagy Axis Pubmed: 31658172 |
| Journal of Diabetes Research | Middle-Aged Indians with Type 2 Diabetes Are at Higher Risk of Biological Ageing with Special Reference to Serum CDKN2A Pubmed: 32280716 |
| Acta Biochim Pol | Dietary Lactobacillus plantarum LS/07 and inulin in the management of chronic disease risk factors Pubmed: 33108141 |
| Ecotoxicol Environ Saf | Permethrin induced arterial retention of native and oxidized LDL in rats by promoting inflammation, oxidative stress and affecting LDL receptors, and collagen … Pubmed: 32911180 |
| Cell Death & Disease | Oxidized-LDL inhibits testosterone biosynthesis by affecting mitochondrial function and the p38 MAPK/COX-2 signaling pathway in Leydig cells Pubmed: 32796811 |
| Nutrients | Ginseng-Induced Changes to Blood Vessel Dilation and the Metabolome of Rats Pubmed: 32727012 |
| INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | The Fungal Iron Chelator Desferricoprogen Inhibits Atherosclerotic Plaque Formation Pubmed: 32635347 |
| Oxidative Medicine and Cellular Longevity | Improvement of Endothelial Dysfunction of Berberine in Atherosclerotic Mice and Mechanism Exploring through TMT-Based Proteomics Pubmed: 32566106 |
| JOURNAL OF CELLULAR AND MOLECULAR MEDICINE | The microvesicle/CD36 complex triggers a prothrombotic phenotype in patients with non‐valvular atrial fibrillation Pubmed: 32510852 |
| Antioxidants | Nicotinamide Prevents Apolipoprotein B-Containing Lipoprotein Oxidation, Inflammation and Atherosclerosis in Apolipoprotein E-Deficient Mice 33233455 |
| MOLECULAR NUTRITION & FOOD RESEARCH | Digestive n©\6 Lipid Oxidation, a Key Trigger of Vascular Dysfunction and Atherosclerosis in the Western Diet: Protective Effects of Apple Polyphenols 33450108 |
| Scientific reports | Genetic deletion of Abcc6 disturbs cholesterol homeostasis in mice 33483533 |
| Scientific reports | Antibodies against apoB100 peptide 210 inhibit atherosclerosis in apoE-/-mice 33907226 |
| Journal of Food and Nutrition Research | Effect of Hibiscus sabdariffa Tea Extracts Intake on the Atherosclerosis Biomarkers in Adults |
| Journal of Food and Nutrition Research | Regulation of CD36 Gene Expression by Hibiscus Sabdariffa Tea Extracts to Affect the Atherosclerosis Biomarkers in Saudi Women |
| Eur J Nutr | The effects of long-term moderate exercise and Western-type diet on oxidative/nitrosative stress, serum lipids and cytokines in female Sprague Dawley rats 34319428 |
| Pediatr Gastroenterol Hepatol Nutr | The Relationship between Daily Fructose Consumption and Oxidized Low-Density Lipoprotein and Low-Density Lipoprotein Particle Size in Children with … 34557400 |
| J Mol Cell Cardiol | ILF3 is responsible for hyperlipidemia-induced arteriosclerotic calcification by mediating BMP2 and STAT1 transcription 34343541 |
| J Basic Clin Physiol Pharmacol | Anti-atherosclerotic activity of aqueous extract of Ipomoea batatas (L.) leaves in high-fat diet-induced atherosclerosis model rats Pubmed:34986543 |
| Oxid Med Cell Longev | DNA Methyltransferase 3b Accelerates the Process of Atherosclerosis Pubmed:35422896 |
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