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ELISA Kit DIY Materials for Fibroblast Growth Factor 2, Basic (FGF2)

B-FGF; BFGF; FGFB; HBGH-2; Basic Fibroblast Growth Factor; Heparin-binding growth factor 2

  • Product No.KSA551Ra01
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range62.5-4000pg/mL
  • Applicable Sensitivity
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • DownloadInstruction Manual
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1094  US$ 1824  US$ 3283  US$ 6384  US$ 10944 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Fibroblast Growth Factor 2, Basic (FGF2). No significant cross-reactivity or interference between Fibroblast Growth Factor 2, Basic (FGF2) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Journal of Pediatric Surgery The influence of nutrients, biliary-pancreatic secretions, and systemic trophic hormones on intestinal adaptation in a Roux-en-Y bypass model PubMed: 20438940
International Journal of Nanomedicine Effect of heparan sulfate and gold nanoparticles on muscle development during embryogenesis PubMed: PMC3254262
Archives of Animal Nutrition Effect of taurine and gold nanoparticles on the morphological and molecular characteristics of muscle development during chicken embryogenesis Tandfonline: 644918
Diabetes Peroxisome Proliferator–Activated Receptor-γ Coactivator-1α (PGC-1α) Enhances Engraftment and Angiogenesis of Mesenchymal Stem Cells in Diabetic Hindlimb Ischemia Pubmed: 22266669
Molecular Pharmacology Electrospun Fibers with Plasmid bFGF Polyplex Loadings Promote Skin Wound Healing in Diabetic Rats Pubmed: 22091745
Science Alert Antiangiogenic Activities of Cinnamon, Black and Green Tea Extracts on Experimentally Induced Breast Cancer in Rats Scialert: Source
Nanoscale Research Letters Silver nanoparticles administered to chicken affect VEGFA and FGF2 gene expression in breast muscle and heart. PubMed: 22827927
PLoS One Carbon nanoparticles downregulate expression of basic fibroblast growth factor in the heart during embryogenesis PubMed: PMC3771850
International Journal of Molecular Sciences Nano-Nutrition of Chicken Embryos. The Effect of in Ovo Administration of Diamond Nanoparticles and L-Glutamine on Molecular Responses in Chicken Embryo Pectoral Muscles NCBI:PMC3856104
Archives of Animal Nutrition Nano-nutrition of chicken embryos. The effect of silver nanoparticles and ATP on expression of chosen genes involved in myogenesis Pubmed: 23952606
Journal of Rehabilitation Research & Development Role of sensory and motor intensity of electrical stimulation on fibroblastic growth factor-2 expression, inflammation, vascularization, and mechanical strength of full-thickness wounds Pubmed: 23934870
PLoS One.? Carboxypeptidase E-ΔN, a Neuroprotein Transiently Expressed during Development Protects Embryonic Neurons against Glutamate Neurotoxicity Pubmed:25426952
Implant Dentistry Effects of Hemostatic Agents on Fibroblast Cells Lww:Source
Journal of Materials Chemistry B bFGF-grafted electrospun fibrous scaffolds via poly(dopamine) for skin wound healing Rsc:Source
Archives of Animal Nutrition Effect of silver nanoparticles and hydroxyproline, administered in ovo, on the development of blood vessels and cartilage collagen structure in chicken embryos Pubmed:25530495
Int J Clin Exp Med. The effects of self-assembling peptide RADA16 hydrogel on malignant phenotype of human hepatocellular carcinoma cell PubMed: 26628972
Theriogenology Evaluation of Changes in Doppler Ultrasonography Indices and Levels of Maternal Serum Angiogenic Factors throughout Pregnancy in Ewes Science: Article
Tumor Biology Hypoxia-induced secretion of IL-10 from adipose-derived mesenchymal stem cell promotes growth and cancer stem cell properties of Burkitt lymphoma PubMed: 26695151
Acta Biomaterialia Preparation and characterization of pro-angiogenic gel derived from small intestinal submucosa article:S1742706115301410
22 Surface biofunctional drug-loaded electrospun fibrous scaffolds for comprehensive repairing hypertrophic scars Pubmed:26774564
Genet Mol Res Changes in growth factor levels in the cerebrospinal fluid of autism patients after transplantation of human umbilical cord blood mononuclear cells and umbilical cord-derived mesenchymal stem cells Pubmed:27323064
BMC Neuroscience  3-O-Methyldopa inhibits astrocyte-mediated dopaminergic neuroprotective effects of l-DOPA pubmed:27456338
Medical Science Monitor 5-Aminolaevulinic Acid-Based Photodynamic Therapy Restrains Pathological Hyperplasia of Fibroblasts 898221
Biomedicine & Pharmacotherapy FGF-2-mediated FGFR1 signaling in human microvascular endothelial cells is activated by vaccarin to promote angiogenesis pubmed:28841454
Medical Science Monitor 5-Aminolaevulinic Acid-Based Photodynamic Therapy Restrains Pathological Hyperplasia of Fibroblasts pubmed:28052053
International Journal of Clinical and Experimental Pathology Platelet-derived growth factor and stromal cell-derived factor-1 promote the skin wound repairing effect of bone mesenchymal stem cells: a key role of matrix metalloproteinase 1 and collagens ISSN:1936-2625/IJCEP0058424
Journal of Biomaterials and Tissue Engineering The Combination of Acellular Porcine Small Intestinal Submucosa (SIS) Cryogel Granules and Adipose Tissue Improves the Survival Ratio of Fat Grafting in Mice 10.1166/jbt.2017.1652
Stem Cells and Development Lower Senescence of Adipose-Derived Stem Cells than Donor-Matched Bone Marrow Stem Cells for Surgical Ventricular Restoration Pubmed:29630447
Neuroscience Letters Increased serum FGF2 levels in first-episode, drug-free patients with schizophrenia Pubmed: 30172685
Biochemistry and Cell Biology Silencing of Astrocyte elevated gene-1 (AEG-1) inhibits proliferation, migration and invasion, and promotes apoptosis in pancreatic cancer cells Pubmed: 30359541
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International Journal of Ophthalmology A novel xeno-free culture system for human retinal pigment epithelium cells Pubmed: 31024807
Open Access Macedonian Journal of Medical Sciences Low Degree Hyaluronic Acid Crosslinking Inducing the Release of TGF-Β1 in Conditioned Medium of Wharton's Jelly-Derived Stem Cells
Chemico-Biological Interactions Bisphenol S triggers the malignancy of hemangioma cells via regulation of basic fibroblast growth factor Pubmed: 31669319
International Journal of Molecular Sciences β-Galactosylceramidase Deficiency Causes Bone Marrow Vascular Defects in an Animal Model of Krabbe Disease Pubmed: 31905906
Molecular Medicine Reports Long non‑coding RNA NORAD regulates angiogenesis of human umbilical vein endothelial cells via miR‑590‑3p under hypoxic conditions Pubmed: 32323787
Basic Fibroblast Growth Factor is Involved in Bisphenol S Induced Proliferation of Hemangioma Cells
Bioact Mater New design to remove leukocytes from platelet-rich plasma (PRP) based on cell dimension rather than density 33842739
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World J Hepatol Leukocyte cell-derived chemotaxin-2 and fibroblast growth factor 21 in alcohol-induced liver cirrhosis Pubmed:35070009
Biomaterials Advances Human adipose-derived stem cell-loaded small intestinal submucosa as a bioactive wound dressing for the treatment of diabetic wounds in rats
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KSA551Ra01 ELISA Kit DIY Materials for Fibroblast Growth Factor 2, Basic (FGF2) Main materials for "Do It (ELISA Kit) Yourself".