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ELISA Kit DIY Materials for Granzyme A (GZMA)

CTLA3; HFSP; HF; H factor; Granzyme 1; Fragmentin-1; Hanukkah factor; Cytotoxic T-Lymphocyte-Associated Serine Esterase 3; CTL tryptase; Cytotoxic T-lymphocyte proteinase 1

  • Product No.KSA599Mu01
  • Organism SpeciesMus musculus (Mouse) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range78.12-5000pg/mL
  • Applicable Sensitivity35pg/mL
  • ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1166  US$ 1944  US$ 3499  US$ 6804  US$ 11664 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Granzyme A (GZMA). No significant cross-reactivity or interference between Granzyme A (GZMA) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Cell Reports Granzyme A Produces Bioactive IL-1β through a Nonapoptotic Inflammasome-Independent Pathway Pubmed:25437548
Journal of Dermatological Science Relationships among plasma granzyme B level, pruritus and dermatitis in patients with atopicdermatitis. pubmed:27686401
Journal of Maternal-Fetal & Neonatal Medicine Extracellular granzyme A in amniotic fluid is elevated in the presence of sterile intra-amniotic inflammation in preterm prelabor rupture of membranes Pubmed: 32912008
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
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PAA599Mu02 Polyclonal Antibody to Granzyme A (GZMA) WB; IHC; ICC; IP.
PAA599Mu01 Polyclonal Antibody to Granzyme A (GZMA) WB
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SEA599Mu ELISA Kit for Granzyme A (GZMA) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA599Mu Multiplex Assay Kit for Granzyme A (GZMA) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA599Mu01 ELISA Kit DIY Materials for Granzyme A (GZMA) Main materials for "Do It(ELISA Kit) Yourself"