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ELISA Kit DIY Materials for Mothers Against Decapentaplegic Homolog 1 (Smad1)

BSP1; JV4-1; JV41; MADH1; MADR1; SMAD Family Member 1; Mad-related protein 1; Transforming growth factor-beta-signaling protein 1; Mothers against DPP homolog 1

  • Product No.KSA647Mu01
  • Organism SpeciesMus musculus (Mouse) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable Sampletissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.312-20ng/mL
  • Applicable Sensitivity0.125ng/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1296  US$ 2160  US$ 3888  US$ 7560  US$ 12960 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Mothers Against Decapentaplegic Homolog 1 (Smad1). No significant cross-reactivity or interference between Mothers Against Decapentaplegic Homolog 1 (Smad1) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Chem Biol Interact Camel milk attenuates the biochemical and morphological features of diabetic nephropathy: Inhibition of Smad1 and collagen type IV synthesis PubMed: 25617480
Research report EFFECTS OF FLUORIDE ON THE EXPRESSION OF BMP-2 AND SMAD1 IN RAT OSTEOBLASTS IN VITRO files:p013-022
Phytomedicine Efficacy of Total flavonoids of Rhizoma drynariae on the Blood Vessels and the Growth Quality of Bone Graft in the Induced Membrane Pubmed:35278899
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPA647Mu01 Recombinant Mothers Against Decapentaplegic Homolog 1 (Smad1) Positive Control; Immunogen; SDS-PAGE; WB.
PAA647Mu01 Polyclonal Antibody to Mothers Against Decapentaplegic Homolog 1 (Smad1) WB; IHC; ICC; IP.
SEA647Mu ELISA Kit for Mothers Against Decapentaplegic Homolog 1 (Smad1) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA647Mu Multiplex Assay Kit for Mothers Against Decapentaplegic Homolog 1 (Smad1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA647Mu01 ELISA Kit DIY Materials for Mothers Against Decapentaplegic Homolog 1 (Smad1) Main materials for "Do It (ELISA Kit) Yourself".