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ELISA Kit DIY Materials for Osteonectin (ON)

SPARC; BM-40; Secreted Protein,Acidic,Cysteine-Rich; Basement-membrane protein 40; Secreted protein acidic and rich in cysteine

  • Product No.KSA791Mu01
  • Organism SpeciesMus musculus (Mouse) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.156-10ng/mL
  • Applicable Sensitivity0.059ng/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1166  US$ 1944  US$ 3499  US$ 6804  US$ 11664 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Osteonectin (ON). No significant cross-reactivity or interference between Osteonectin (ON) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
J Mol Med (Berl) Growth hormone replacement therapy regulates microRNA-29a and targets involved in insulin resistance PubMed: 26199111
Muscle Nerve Effect of resistance ladder training on SPARC expression in skeletal muscle of hindlimb immobilized rats PubMed: 26467437
Medicine (Baltimore) An Attempt to Evaluate Selected Aspects of “Bone–Fat Axis” Function in Healthy Individuals and Patients With Pancreatic Cancer PubMed: 26266370
British Journal of Cancer A proteomics-based approach identifies secreted protein acidic and rich in cysteine as a prognostic biomarker in malignant pleural mesothelioma Pubmed:26889976
Journal of diabetes and its complications Associations between FGF21, osteonectin and bone turnover markers in type 2 diabetic patients with albuminuria. pubmed:27916484
American journal of the medical sciences Association of Bone Metabolic Markers With Diabetic Retinopathy and Diabetic Macular Edema in Elderly Chinese Individuals With Type 2 Diabetes Mellitus pubmed:29078839
Translational Research in Oral Oncology Testican 1 (SPOCK1) and protein tyrosine phosphatase, receptor type S (PTPRS) show significant increase in saliva of tobacco users with oral cancer Doi: 10.1177/2057178X18800534
[ENGINEERING OF BIOMATERIALS Osteoconductive potential of PLGA/bioglass composite biomaterials
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPA791Mu01 Recombinant Osteonectin (ON) Positive Control; Immunogen; SDS-PAGE; WB.
RPA791Mu02 Recombinant Osteonectin (ON) Positive Control; Immunogen; SDS-PAGE; WB.
PAA791Mu01 Polyclonal Antibody to Osteonectin (ON) WB; IHC; ICC; IP.
PAA791Mu02 Polyclonal Antibody to Osteonectin (ON) WB; IHC; ICC; IP.
LAA791Mu81 FITC-Linked Polyclonal Antibody to Osteonectin (ON) WB; IHC; ICC; IF.
LAA791Mu71 Biotin-Linked Polyclonal Antibody to Osteonectin (ON) WB; IHC; ICC.
SEA791Mu ELISA Kit for Osteonectin (ON) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA791Mu Multiplex Assay Kit for Osteonectin (ON) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA791Mu01 ELISA Kit DIY Materials for Osteonectin (ON) Main materials for "Do It (ELISA Kit) Yourself".