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ELISA Kit DIY Materials for Bone Morphogenetic Protein 7 (BMP7)

OP1; Osteogenic Protein-1; Eptotermin alfa

  • Product No.KSA799Ra01
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range15.6-1000pg/mL
  • Applicable Sensitivity5.9pg/mL
  • ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1231  US$ 2052  US$ 3694  US$ 7182  US$ 12312 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Bone Morphogenetic Protein 7 (BMP7). No significant cross-reactivity or interference between Bone Morphogenetic Protein 7 (BMP7) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
PLoS ONE A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches Plosone: Source
BioMed Research International Serum Heme Oxygenase-1 and BMP-7 Are Potential Biomarkers for Bone Metabolism in Patients with Rheumatoid Arthritis and Ankylosing Spondylitis Pubmed:27314037
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY The dose-time effects of fluoride on the expression and DNA methylation level of the promoter region of BMP-2 and BMP-7 in rats Pubmed: 32004919
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Temporal TGF-β Supergene Family Signalling Cues Modulating Tissue Morphogenesis: Chondrogenesis within a Muscle Tissue Model? Pubmed: 32660137
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPA799Ra01 Recombinant Bone Morphogenetic Protein 7 (BMP7) Positive Control; Immunogen; SDS-PAGE; WB.
PAA799Ra01 Polyclonal Antibody to Bone Morphogenetic Protein 7 (BMP7) WB; IHC; ICC; IP.
MAA799Ra21 Monoclonal Antibody to Bone Morphogenetic Protein 7 (BMP7) WB; IHC; ICC; IP.
LAA799Ra72 Biotin-Linked Monoclonal Antibody to Bone Morphogenetic Protein 7 (BMP7) WB; IHC; ICC.
SEA799Ra ELISA Kit for Bone Morphogenetic Protein 7 (BMP7) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA799Ra Multiplex Assay Kit for Bone Morphogenetic Protein 7 (BMP7) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
PSA799Ra01 Antibody Pair for Bone Morphogenetic Protein 7 (BMP7) ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSA799Ra01 ELISA Kit DIY Materials for Bone Morphogenetic Protein 7 (BMP7) Main materials for "Do It(ELISA Kit) Yourself"