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ELISA Kit DIY Materials for Heat Shock Protein 60 (Hsp60)

HSPD1; GROEL; CPN60; HuCHA60; Heat Shock 60kD Protein 1, Chaperonin; Spastic Paraplegia 13,Autosomal Dominant; 60 kDa chaperonin; Mitochondrial matrix protein P1; P60 lymphocyte protein

  • Product No.KSA822Mu01
  • Organism SpeciesMus musculus (Mouse) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range1.56-100ng/mL
  • Applicable Sensitivity0.59ng/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1166  US$ 1944  US$ 3499  US$ 6804  US$ 11664 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Heat Shock 60kD Protein 1, Chaperonin (HSPD1). No significant cross-reactivity or interference between Heat Shock 60kD Protein 1, Chaperonin (HSPD1) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Sci Rep Hsp60 response in experimental and human temporal lobe epilepsy PubMed: 25801186
Cellular and Molecular Gastroenterology and Hepatology Senescent hepatocytes in decompensated liver show reduced UPR and its key player, CLPP, attenuates senescence in vitro Pubmed: 30878663
Breast Cancer Res Treat Activation of mitochondrial unfolded protein response is associated with Her2-overexpression breast cancer Pubmed: 32601970
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPA822Mu01 Recombinant Heat Shock Protein 60 (Hsp60) Positive Control; Immunogen; SDS-PAGE; WB.
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PAA822Mu01 Polyclonal Antibody to Heat Shock Protein 60 (Hsp60) WB; IHC; ICC; IP.
PAA822Mu02 Polyclonal Antibody to Heat Shock Protein 60 (Hsp60) WB; IHC; ICC; IP.
SEA822Mu ELISA Kit for Heat Shock Protein 60 (Hsp60) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA822Mu CLIA Kit for Heat Shock Protein 60 (Hsp60) Chemiluminescent immunoassay for Antigen Detection.
LMA822Mu Multiplex Assay Kit for Heat Shock Protein 60 (Hsp60) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA822Mu01 ELISA Kit DIY Materials for Heat Shock Protein 60 (Hsp60) Main materials for "Do It (ELISA Kit) Yourself".