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ELISA Kit DIY Materials for Heat Shock Protein 60 (Hsp60)

HSPD1; GROEL; CPN60; HuCHA60; Heat Shock 60kD Protein 1, Chaperonin; Spastic Paraplegia 13,Autosomal Dominant; 60 kDa chaperonin; Mitochondrial matrix protein P1; P60 lymphocyte protein

  • Product No.KSA822Ra01
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids..
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range1.56-100ng/mL
  • Applicable Sensitivity0.65ng/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1231  US$ 2052  US$ 3694  US$ 7182  US$ 12312 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Heat Shock 60kD Protein 1, Chaperonin (HSPD1). No significant cross-reactivity or interference between Heat Shock 60kD Protein 1, Chaperonin (HSPD1) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Sci Rep Hsp60 response in experimental and human temporal lobe epilepsy PubMed: 25801186
Cellular and Molecular Gastroenterology and Hepatology Senescent hepatocytes in decompensated liver show reduced UPR and its key player, CLPP, attenuates senescence in vitro Pubmed: 30878663
Breast Cancer Res Treat Activation of mitochondrial unfolded protein response is associated with Her2-overexpression breast cancer Pubmed: 32601970
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPA822Ra01 Recombinant Heat Shock Protein 60 (Hsp60) Positive Control; Immunogen; SDS-PAGE; WB.
PAA822Ra01 Polyclonal Antibody to Heat Shock Protein 60 (Hsp60) WB; IHC; ICC; IP.
MAA822Ra21 Monoclonal Antibody to Heat Shock Protein 60 (Hsp60) WB; IHC; ICC; IP.
SEA822Ra ELISA Kit for Heat Shock Protein 60 (Hsp60) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA822Ra Multiplex Assay Kit for Heat Shock Protein 60 (Hsp60) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA822Ra01 ELISA Kit DIY Materials for Heat Shock Protein 60 (Hsp60) Main materials for "Do It (ELISA Kit) Yourself".