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ELISA Kit DIY Materials for Retinol Binding Protein 4 (RBP4)

PRBP; RBP; Plasma retinol-binding protein; Retinol Binding Protein 4, Plasma

  • Product No.KSA929Ga01
  • Organism SpeciesChicken (Gallus) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.625-40ng/mL
  • Applicable Sensitivity0.292ng/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1102  US$ 1837  US$ 3307  US$ 6430  US$ 11022 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Retinol Binding Protein 4, Plasma (RBP4). No significant cross-reactivity or interference between Retinol Binding Protein 4, Plasma (RBP4) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Diabetes Technology & Therapeutics Proteomic Identification of Human Urinary Biomarkers in Diabetes Mellitus Type 2 Liebert: 20100078
Journal of Pharmaceutical and Biomedical Analysis Effect of high dose thiamine on the levels of urinary protein biomarkers in diabetes mellitus type 2 PubMed: 21130593
Evid Based Complement Alternat Med Quercetin Protects against Cadmium-Induced Renal Uric Acid Transport System Alteration and Lipid Metabolism Disorder in Rats PubMed: PMC3368504
Journal of Endocrinology Combined galanin with insulin improves insulin sensitivity of diabetic rat muscles Pubmed:24501381
J Endocrinol Central alarin ameliorated insulin resistance of adipocytes in type 2 diabetic rats Pubmed:25240061
Peptides Effect of endogenous galanin on glucose transporter 4 expression in cardiac muscle of type 2 diabetic rats Pubmed:25445608
Obesity Surgery Long-term Effect of Ileal Transposition on Adipokine Serum Level in Zucker (Orl)-Lepr fa Fatty Rats Pubmed:25697126
Clin Exp Nephrol Differentially expressed urinary biomarkers in children with idiopathic nephrotic syndrome PubMed: 26351173
Experimental and Therapeutic Medicine Expression of retinol binding protein 4 and nuclear factor-ÎşB in diabetic rats with atherosclerosis and the intervention effect of pioglitazone Pubmed:27446311
Experimental and Therapeutic Medicine Expression of retinol binding protein 4 and nuclear factor-κB in diabetic rats with atherosclerosisand the intervention effect of pioglitazone. pubmed:27446311
Journal of Chromatography A Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosis S0021967317311858
BMC Cancer Screening for immune-potentiating antigens from hepatocellular carcinoma patients after radiofrequency ablation by serum proteomic analysis Pubmed:29386009
Nutrition & Diabetes Lack of pronounced changes in the expression of fatty acid handling proteins in adipose tissue and plasma of morbidly obese humans Pubmed:29335416
Molecular Medicine Reports RBP4 regulates trophoblastic cell proliferation and invasion via the PI3K/AKT signaling pathway Pubmed:30015949
Relationship between retinol and risk of diabetic retinopathy: a case-control study
Scientific Reports Retinol binding protein 4 abundance in plasma and tissues is related to body fat deposition in cattle Pubmed: 31147589
Potassium supplementation blunts the effects of high salt intake on serum retinol‐binding protein 4 levels in healthy individuals
Docosahexaenoic acid (DHA), a nutritional supplement, modulates steroid insensitivity in asthma
Eur J Nutr The dietary inflammatory index is associated with anti-and pro-inflammatory adipokines in Brazilian schoolchildren 33575861
Eur J Pediatr Pro-and anti-inflammatory adipokines are associated with cardiometabolic risk markers in Brazilian schoolchildren 33834274
Sci Rep Ileal transposition helps to regulate plasma hepatokine levels in obese Zucker (Crl: ZUC (ORL)-Lepr fa) rats 33833309
Int Arch Occup Environ Health Biomarkers of cadmium exposure and renal function in estuarine adult villagers 34773507
Catalog No. Related products for research use of Chicken (Gallus) Organism species Applications (RESEARCH USE ONLY!)
APA929Ga01 Active Retinol Binding Protein 4 (RBP4) Cell culture; Activity Assays.
RPA929Ga01 Recombinant Retinol Binding Protein 4 (RBP4) Positive Control; Immunogen; SDS-PAGE; WB.
PAA929Ga01 Polyclonal Antibody to Retinol Binding Protein 4 (RBP4) WB; IHC; ICC; IP.
LAA929Ga71 Biotin-Linked Polyclonal Antibody to Retinol Binding Protein 4 (RBP4) WB; IHC; ICC.
LAA929Ga81 FITC-Linked Polyclonal Antibody to Retinol Binding Protein 4 (RBP4) WB; IHC; ICC; IF.
SEA929Ga ELISA Kit for Retinol Binding Protein 4 (RBP4) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA929Ga Multiplex Assay Kit for Retinol Binding Protein 4 (RBP4) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA929Ga01 ELISA Kit DIY Materials for Retinol Binding Protein 4 (RBP4) Main materials for "Do It (ELISA Kit) Yourself".