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ELISA Kit DIY Materials for Retinol Binding Protein 4 (RBP4)
PRBP; RBP; Plasma retinol-binding protein; Retinol Binding Protein 4, Plasma
- Product No.KSA929Mu01
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range0.312-20ng/mL
- Applicable Sensitivity0.142ng/mL
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
- Downloadn/a
- UOM 96T*596T*10 96T*20 96T*50 96T*100
- FOB
US$ 824
US$ 1374
US$ 2473
US$ 4809
US$ 8244
For more details, please contact local distributors!
SPECIFITY
The Abs in the kit have high sensitivity and excellent specificity for detection of Retinol Binding Protein 4, Plasma (RBP4).No significant cross-reactivity or interference between Retinol Binding Protein 4, Plasma (RBP4) and analogues was observed.
USAGE
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
STORAGE
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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| Magazine | Citations |
| Diabetes Technology & Therapeutics | Proteomic Identification of Human Urinary Biomarkers in Diabetes Mellitus Type 2 Liebert: 20100078 |
| Journal of Pharmaceutical and Biomedical Analysis | Effect of high dose thiamine on the levels of urinary protein biomarkers in diabetes mellitus type 2 PubMed: 21130593 |
| Evid Based Complement Alternat Med | Quercetin Protects against Cadmium-Induced Renal Uric Acid Transport System Alteration and Lipid Metabolism Disorder in Rats PubMed: PMC3368504 |
| Journal of Endocrinology | Combined galanin with insulin improves insulin sensitivity of diabetic rat muscles Pubmed:24501381 |
| J Endocrinol | Central alarin ameliorated insulin resistance of adipocytes in type 2 diabetic rats Pubmed:25240061 |
| Peptides | Effect of endogenous galanin on glucose transporter 4 expression in cardiac muscle of type 2 diabetic rats Pubmed:25445608 |
| Obesity Surgery | Long-term Effect of Ileal Transposition on Adipokine Serum Level in Zucker (Orl)-Lepr fa Fatty Rats Pubmed:25697126 |
| Clin Exp Nephrol | Differentially expressed urinary biomarkers in children with idiopathic nephrotic syndrome PubMed: 26351173 |
| Experimental and Therapeutic Medicine | Expression of retinol binding protein 4 and nuclear factor-ÎşB in diabetic rats with atherosclerosis and the intervention effect of pioglitazone Pubmed:27446311 |
| Experimental and Therapeutic Medicine | Expression of retinol binding protein 4 and nuclear factor-κB in diabetic rats with atherosclerosisand the intervention effect of pioglitazone. pubmed:27446311 |
| Journal of Chromatography A | Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosis S0021967317311858 |
| BMC Cancer | Screening for immune-potentiating antigens from hepatocellular carcinoma patients after radiofrequency ablation by serum proteomic analysis Pubmed:29386009 |
| Nutrition & Diabetes | Lack of pronounced changes in the expression of fatty acid handling proteins in adipose tissue and plasma of morbidly obese humans Pubmed:29335416 |
| Molecular Medicine Reports | RBP4 regulates trophoblastic cell proliferation and invasion via the PI3K/AKT signaling pathway Pubmed:30015949 |
| Relationship between retinol and risk of diabetic retinopathy: a case-control study | |
| Scientific Reports | Retinol binding protein 4 abundance in plasma and tissues is related to body fat deposition in cattle Pubmed: 31147589 |
| Potassium supplementation blunts the effects of high salt intake on serum retinol‐binding protein 4 levels in healthy individuals | |
| Docosahexaenoic acid (DHA), a nutritional supplement, modulates steroid insensitivity in asthma | |
| Eur J Nutr | The dietary inflammatory index is associated with anti-and pro-inflammatory adipokines in Brazilian schoolchildren 33575861 |
| Eur J Pediatr | Pro-and anti-inflammatory adipokines are associated with cardiometabolic risk markers in Brazilian schoolchildren 33834274 |
| Sci Rep | Ileal transposition helps to regulate plasma hepatokine levels in obese Zucker (Crl: ZUC (ORL)-Lepr fa) rats 33833309 |
| Int Arch Occup Environ Health | Biomarkers of cadmium exposure and renal function in estuarine adult villagers 34773507 |