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ELISA Kit DIY Materials for Cyclophilin A (CYPA)

PPIA; CyP-A; CYPH; Peptidylprolyl Isomerase A; Peptidyl-Prolyl Isomerase A; Peptidyl-prolyl cis-trans isomerase A; Cyclosporin A-binding protein

  • Product No.KSA979Ra01
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable Sampleserum, plasma, tissue homogenates and other biological fluids
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.156-10ng/mL
  • Applicable Sensitivity0.064ng/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1231  US$ 2052  US$ 3694  US$ 7182  US$ 12312 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Cyclophilin A (CYPA).No significant cross-reactivity or interference between Cyclophilin A (CYPA) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Atherosclerosis EMMPRIN and its ligand cyclophilin A regulate MT1-MMP, MMP-9 and M-CSF during foam cell formation PubMed: 19758589
Biomarkers Cyclophilin-A: a novel biomarker for untreated male essential hypertension Pubmed: 24164594
Cardiovascular Diabetology Plasma level of cyclophilin A is increased in patients with type 2 diabetes mellitus and suggests presence of vascular disease Pubmed:24502618
International Journal of Biological Sciences Decrease in Plasma Cyclophilin A Concentration at 1 Month after Myocardial Infarction Predicts Better Left Ventricular Performance and Synchronicity at 6 Months: A Pilot Study in Patients with ST Elevation Myocardial Infarction Pubmed:25552928
Medicine Cyclophilin A in Ruptured Intracranial Aneurysm PubMed: 26426668
Mol Cell Biochem PPIA rs6850: A> G single-nucleotide polymorphism is associated with raised plasma cyclophilin A levels in patients with coronary artery disease PubMed: 26702934
Medicine (Baltimore) Urinary Cyclophilin A as a New Marker for Diabetic Nephropathy: A Cross-Sectional Analysis of Diabetes Mellitus PubMed: 26496315
International Journal of Cardiology Cyclophilin A modulates bone marrow-derived CD117(+) cells and enhances ischemia-induced angiogenesisvia the SDF-1/CXCR4 axis. Pubmed:27057951
Clinica Chimica Acta Novel findings of secreted cyclophilin A in diabetic nephropathy and its association with renal protection of dipeptidyl peptidase 4 inhibitor pubmed:27823952
Cardiovascular Diabetology Cyclophilin A enhances macrophage differentiation and lipid uptake in high glucose conditions: a cellular mechanism for accelerated macro vascular disease in diabetes mellitus. pubmed:27809851
Clinical Science Metformin attenuates effects of cyclophilin A on macrophages, reduces lipid uptake and secretion of cytokines by repressing decreased AMPK activity Pubmed:29382697
Journal of Molecular Histology Cyclophilin a increases CD68 cell infiltration in rat experimental periodontitis Pubmed:29318411
Free Radical Research Cyclophilin A and CD147 associate with progression of diabetic nephropathy Pubmed: 30572748
The Kaohsiung Journal of Medical Sciences Association of cyclophilin A level and pulse pressure in predicting recurrence of cerebral infarction Pubmed: 31670477
Cells. Cyclophilin A/EMMPRIN Axis Is Involved in Pro-Fibrotic Processes Associated with Thoracic Aortic Aneurysm of Marfan Syndrome Patients Pubmed: 31936351
JAMA Neurology APPENDIX A: Relationship between cyclophilin A levels and matrix metalloproteinase-9 activity in cerebrospinal fluid of cognitively normal apolipoprotein E4 carriers … Pubmed: 24030206
Menoufia Medical Journal Urinary podocalyxin and cyclophilin A: markers for early detection of type 2 diabetic nephropathy
Toxins Evaluation of the Therapeutic Effects of Protocatechuic Aldehyde in Diabetic Nephropathy 34437430
Cell Death & Disease Proteomic analysis reveals USP7 as a novel regulator of palmitic acid-induced hepatocellular carcinoma cell death Pubmed:35732625
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
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MAA979Ra25 Monoclonal Antibody to Cyclophilin A (CYPA) WB; IHC; ICC; IP.
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KSA979Ra01 ELISA Kit DIY Materials for Cyclophilin A (CYPA) Main materials for "Do It (ELISA Kit) Yourself".