ELISA Kit DIY Materials for Apolipoprotein B (APOB)

Apo-B; FLDB; Apo B-100; Apo B-48

  • Product No.KSC003Ra01
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range6.25-400ng/mL
  • Applicable Sensitivity2.92ng/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1368  US$ 2280  US$ 4104  US$ 7980  US$ 13680 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Apolipoprotein B (APOB). No significant cross-reactivity or interference between Apolipoprotein B (APOB) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Journal of Lipid Research Group 1B phospholipase A2 deficiency protects against diet-induced hyperlipidemia in mice Jlr: M019463
J Clin Endocrinol Metab.? Elevated retinol binding protein 4 induces apolipoprotein B production and associates with hypertriglyceridemia Pubmed:25781360
Biochim Biophys Acta Culturing of HepG2 cells with human serum improve their functionality and suitability in studies of lipid metabolism PubMed: 26515253
Am J Cardiovasc Dis Hypoascorbemia induces atherosclerosis and vascular deposition of lipoprotein (a) in transgenic mice PubMed: 26064792
The Journal of Biological Chemistry Proprotein convertase subtilisin/Kexin type 9 (PCSK9) single domain antibodies are potent inhibitors of LDL receptor degradation Pubmed:27284008
United States Patent Application COMPOSITIONS AND METHODS FOR EFFICACIOUS AND SAFE DELIVERY OF SIRNA USING SPECIFIC CHITOSAN-BASED NANOCOMPLEXES y2016:0152988.html
alcohol and alcoholism Hops (Humulus lupulus) Content in Beer Modulates Effects of Beer on the Liver After Acute Ingestion in Female Mice. pubmed:27659607
Frontiers in Physiology Change in the Lipid Transport Capacity of the Liver and Blood during Reproduction in Rats pubmed:28798692
Food Research International Wild Lonicera caerulea berry polyphenol extract reduces cholesterol accumulation and enhances antioxidant capacity in vitro and in vivo Pubmed:29580541
Journal of The Science of Food and Agriculture Consumption of orange fermented beverage improves antioxidant status and reduces peroxidation lipid and inflammatory markers in healthy humans Pubmed:29124773
European Journal of Pharmacology The effect of exenatide (a GLP-1 analog) and sitagliptin (a DPP-4 inhibitor) on plasma platelet-activating factor acetylhydrolase (PAF-AH) activity and concentration in?… Pubmed: 30768981
Nature Communications Hepatic HuR modulates lipid homeostasis in response to high-fat diet Pubmed: 32546794
Study of in Vivo Serum Lipid Regulation with Ulmus macrocarpa Hance Extract in Rats
Nat Commun GP73 is a TBC-domain Rab GTPase-activating protein contributing to the pathogenesis of non-alcoholic fatty liver disease without obesity 34853313
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPC003Ra02 Recombinant Apolipoprotein B (APOB) Positive Control; Immunogen; SDS-PAGE; WB.
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MAC003Ra22 Monoclonal Antibody to Apolipoprotein B (APOB) WB; IHC; ICC; IP.
MAC003Ra24 Monoclonal Antibody to Apolipoprotein B (APOB) WB; IHC; ICC; IP.
LAC003Ra72 Biotin-Linked Monoclonal Antibody to Apolipoprotein B (APOB) WB; IHC; ICC.
SEC003Ra ELISA Kit for Apolipoprotein B (APOB) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC003Ra Multiplex Assay Kit for Apolipoprotein B (APOB) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
PSC003Ra01 Antibody Pair for Apolipoprotein B (APOB) ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSC003Ra01 ELISA Kit DIY Materials for Apolipoprotein B (APOB) Main materials for "Do It (ELISA Kit) Yourself".