Packages (Simulation)
Image (I)
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DAB staining on IHC-P; Samples: Rat Liver Tissue; Primary Ab: 10µg/ml Rabbit Anti-Rat APOB Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
Image (II)
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Western Blot; Sample: Lane1: Rat Serum; Lane2: HepG2 cell lysate Primary Ab: 1µg/ml Rabbit Anti-Rat APOB Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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Instruction Manual
Certificate
Polyclonal Antibody to Apolipoprotein B (APOB)
Apo-B; FLDB; Apo B-100; Apo B-48
- Product No.PAC003Ra02
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPC003Ra01-Recombinant Apolipoprotein B (APOB)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration500µg/mL
- Organism Species MoreHomo sapiens (Human)
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies. - DownloadInstruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
- FOB
US$ 114
US$ 266
US$ 380
US$ 950
US$ 3800
For more details, please contact local distributors!
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against APOB. It has been selected for its ability to recognize APOB in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.5-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
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Antibody Labeling Customized Service
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Protein A/G Purification Column
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Staining Solution for Cells and Tissue
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Western Blot (WB) Experiment Service
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Immunohistochemistry (IHC) Experiment Service
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Immunocytochemistry (ICC) Experiment Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Long-arm Biotin Labeling Kit
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Real Time PCR Experimental Service
| Magazine | Citations |
| Journal of Lipid Research | Group 1B phospholipase A2 deficiency protects against diet-induced hyperlipidemia in mice Jlr: M019463 |
| J Clin Endocrinol Metab.? | Elevated retinol binding protein 4 induces apolipoprotein B production and associates with hypertriglyceridemia Pubmed:25781360 |
| Biochim Biophys Acta | Culturing of HepG2 cells with human serum improve their functionality and suitability in studies of lipid metabolism PubMed: 26515253 |
| Am J Cardiovasc Dis | Hypoascorbemia induces atherosclerosis and vascular deposition of lipoprotein (a) in transgenic mice PubMed: 26064792 |
| The Journal of Biological Chemistry | Proprotein convertase subtilisin/Kexin type 9 (PCSK9) single domain antibodies are potent inhibitors of LDL receptor degradation Pubmed:27284008 |
| United States Patent Application | COMPOSITIONS AND METHODS FOR EFFICACIOUS AND SAFE DELIVERY OF SIRNA USING SPECIFIC CHITOSAN-BASED NANOCOMPLEXES y2016:0152988.html |
| alcohol and alcoholism | Hops (Humulus lupulus) Content in Beer Modulates Effects of Beer on the Liver After Acute Ingestion in Female Mice. pubmed:27659607 |
| Frontiers in Physiology | Change in the Lipid Transport Capacity of the Liver and Blood during Reproduction in Rats pubmed:28798692 |
| Food Research International | Wild Lonicera caerulea berry polyphenol extract reduces cholesterol accumulation and enhances antioxidant capacity in vitro and in vivo Pubmed:29580541 |
| Journal of The Science of Food and Agriculture | Consumption of orange fermented beverage improves antioxidant status and reduces peroxidation lipid and inflammatory markers in healthy humans Pubmed:29124773 |
| European Journal of Pharmacology | The effect of exenatide (a GLP-1 analog) and sitagliptin (a DPP-4 inhibitor) on plasma platelet-activating factor acetylhydrolase (PAF-AH) activity and concentration in?… Pubmed: 30768981 |
| Nature Communications | Hepatic HuR modulates lipid homeostasis in response to high-fat diet Pubmed: 32546794 |
| Study of in Vivo Serum Lipid Regulation with Ulmus macrocarpa Hance Extract in Rats | |
| Nat Commun | GP73 is a TBC-domain Rab GTPase-activating protein contributing to the pathogenesis of non-alcoholic fatty liver disease without obesity 34853313 |