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ELISA Kit DIY Materials for ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2)

ATP2B; DAR; SERCA2; Calcium pump 2; Endoplasmic reticulum class 1/2 Ca(2+) ATPase; Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle

  • Product No.KSG374Ra01
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.156-10ng/mL
  • Applicable Sensitivity0.055ng/mL
  • ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1327  US$ 2211  US$ 3980  US$ 7739  US$ 13266 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2). No significant cross-reactivity or interference between ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
The Journal of Heart and Lung Transplantation SERCA2a: A potential non-invasive biomarker of cardiac allograft rejection. pubmed:28750934
researchsquare Value of SERCA2a as a Biomarker for the Identification of Patients With Advanced Heart Failure Requiring Circulatory Support
Journal of Personalized Medicine Value of SERCA2a as a Biomarker for the Identification of Patients with Heart Failure Requiring Circulatory Support 34834474
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
SEG374Ra ELISA Kit for ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) Enzyme-linked immunosorbent assay for Antigen Detection.
LMG374Ra Multiplex Assay Kit for ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSG374Ra01 ELISA Kit DIY Materials for ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) Main materials for "Do It(ELISA Kit) Yourself"