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ELISA Kit DIY Materials for Cystatin B (CSTB)

Cys-B; CST6; EPM1; PME; STFB; CPI-B; Stefin B; Liver thiol proteinase inhibitor

  • Product No.KSJ322Ra01
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.312-20ng/mL
  • Applicable Sensitivity0.119ng/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1025  US$ 1708  US$ 3074  US$ 5978  US$ 10248 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Cystatin B (CSTB). No significant cross-reactivity or interference between Cystatin B (CSTB) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
AIDS. Cathepsin B and cystatin B in HIV-seropositive women are associated with infection and HIV-1-associated neurocognitive disorders PubMed: 23291538
International Journal of Circumpolar Health Evaluation of serum procathepsin B, cystatin B and cystatin C as possible biomarkers of ovarian cancer PubMed: PMC3754495
PLoS One. Dysregulation of Macrophage-Secreted Cathepsin B Contributes to HIV-1-Linked Neuronal Apoptosis PubMed: PMC3365072
Journal of Neuroimmune Pharmacology Cocaine potentiates cathepsin B secretion and neuronal apoptosis from HIV-infected macrophages Pubmed:25209871
European Journal of Cancer Supplements A91: Endogenous inhibitors of cysteine proteases and preform of cathepsin B in cancer of reproductive system Science: Article
Annals of Clinical Biochemistry Diagnostic specificity and sensitivity of PIVKAII, GP3, CSTB, SCCA1 and HGF for the diagnosis of hepatocellular carcinoma in patients with alcoholic liver cirrhosis. pubmed:28766361
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPJ322Ra01 Recombinant Cystatin B (CSTB) Positive Control; Immunogen; SDS-PAGE; WB.
PAJ322Ra01 Polyclonal Antibody to Cystatin B (CSTB) WB; IHC; ICC; IP.
LAJ322Ra71 Biotin-Linked Polyclonal Antibody to Cystatin B (CSTB) WB; IHC; ICC.
MAJ322Ra24 Monoclonal Antibody to Cystatin B (CSTB) WB
MAJ322Ra26 Monoclonal Antibody to Cystatin B (CSTB) WB; IHC; ICC; IP.
MAJ322Ra22 Monoclonal Antibody to Cystatin B (CSTB) WB; IHC; ICC; IP.
MAJ322Ra21 Monoclonal Antibody to Cystatin B (CSTB) WB; IHC; ICC; IP.
LAJ322Ra74 Biotin-Linked Monoclonal Antibody to Cystatin B (CSTB) WB; IHC; ICC.
SEJ322Ra ELISA Kit for Cystatin B (CSTB) Enzyme-linked immunosorbent assay for Antigen Detection.
LMJ322Ra Multiplex Assay Kit for Cystatin B (CSTB) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
PSJ322Ra01 Antibody Pair for Cystatin B (CSTB) ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSJ322Ra01 ELISA Kit DIY Materials for Cystatin B (CSTB) Main materials for "Do It (ELISA Kit) Yourself".