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Multiplex Assay Kit for Follistatin (FS) ,etc. by FLIA (Flow Luminescence Immunoassay)
FST; Activin-binding protein
(Note: Up to 8-plex in one testing reaction)
- Product No.LMA391Ra
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length1.5h
- Detection Range0.1-100ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.033 ng/mL.
- DownloadInstruction Manual
- UOM 8Plex 7Plex 6Plex 5Plex 4Plex 3Plex 2Plex1Plex
- FOB
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Result
For more details, please contact local distributors!
Specificity
This assay has high sensitivity and excellent specificity for detection of Follistatin (FS) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Follistatin (FS) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Follistatin (FS) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Follistatin (FS) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 97-105 | 102 |
| EDTA plasma(n=5) | 93-105 | 101 |
| heparin plasma(n=5) | 86-98 | 91 |
| sodium citrate plasma(n=5) | 92-101 | 97 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Follistatin (FS) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Follistatin (FS) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Follistatin (FS) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 78-103% | 94-105% | 83-95% | 99-105% |
| EDTA plasma(n=5) | 96-105% | 92-101% | 79-101% | 93-101% |
| heparin plasma(n=5) | 99-105% | 87-96% | 89-96% | 97-105% |
| sodium citrate plasma(n=5) | 85-92% | 85-99% | 81-99% | 98-105% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:FS) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
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| Magazine | Citations |
| BMC Cancer. | Activins and their related proteins in colon carcinogenesis: insights from early and advanced azoxymethane rat models of colon cancer. pubmed:27835986 |
| Journal of cellular physiology | Role of follistatin in muscle and bone alterations induced by gravity change in mice pubmed:28471505 |
| Scientific Reports | Follistatin expressed in mechanically-damaged salivary glands of male mice induces proliferation of CD49f+ cells Pubmed: 33203957 |
| Pathology & Oncology Research | Profiling Activins and Follistatin in Colorectal Cancer According to Clinical Stage, Tumour Sidedness and Smad4 Status 34867090 |
| Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
| RPA391Ra01 | Recombinant Follistatin (FS) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA391Ra01 | Polyclonal Antibody to Follistatin (FS) | WB; IHC; ICC; IP. |
| MAA391Ra21 | Monoclonal Antibody to Follistatin (FS) | WB; IHC; ICC; IP. |
| CEA391Ra | ELISA Kit for Follistatin (FS) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA391Ra | Multiplex Assay Kit for Follistatin (FS) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |