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ELISA Kit for Follistatin (FS)

FST; Activin-binding protein

Specificity

This assay has high sensitivity and excellent specificity for detection of Follistatin (FS).
No significant cross-reactivity or interference between Follistatin (FS) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Follistatin (FS) and the recovery rates were calculated by comparing the measured value to the expected amount of Follistatin (FS) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 87-96 91
EDTA plasma(n=5) 78-90 86
heparin plasma(n=5) 82-92 87

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Follistatin (FS) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Follistatin (FS) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Follistatin (FS) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 80-95% 91-99% 96-104% 81-103%
EDTA plasma(n=5) 94-103% 92-101% 90-98% 96-103%
heparin plasma(n=5) 97-105% 82-97% 87-101% 79-96%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1 Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Reagent Diluent 1×300µL Stop Solution 1×6mL
TMB Substrate 1×9mL Instruction manual 1
Wash Buffer (30 × concentrate) 1×20mL

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

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Magazine Citations
BMC Cancer. Activins and their related proteins in colon carcinogenesis: insights from early and advanced azoxymethane rat models of colon cancer. pubmed:27835986
Journal of cellular physiology Role of follistatin in muscle and bone alterations induced by gravity change in mice pubmed:28471505
Scientific Reports Follistatin expressed in mechanically-damaged salivary glands of male mice induces proliferation of CD49f+ cells Pubmed: 33203957
Pathology & Oncology Research Profiling Activins and Follistatin in Colorectal Cancer According to Clinical Stage, Tumour Sidedness and Smad4 Status 34867090
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPA391Ra01 Recombinant Follistatin (FS) Positive Control; Immunogen; SDS-PAGE; WB.
PAA391Ra01 Polyclonal Antibody to Follistatin (FS) WB; IHC; ICC; IP.
MAA391Ra21 Monoclonal Antibody to Follistatin (FS) WB; IHC; ICC; IP.
CEA391Ra ELISA Kit for Follistatin (FS) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA391Ra Multiplex Assay Kit for Follistatin (FS) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.