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Multiplex Assay Kit for A Disintegrin And Metalloprotease 17 (ADAM17) ,etc. by FLIA (Flow Luminescence Immunoassay)

CD156b; TACE; CSVP; Tumor Necrosis Factor,Alpha,Converting Enzyme; Disintegrin and metalloproteinase domain-containing protein 17; Snake venom-like protease

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of A Disintegrin And Metalloprotease 17 (ADAM17) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between A Disintegrin And Metalloprotease 17 (ADAM17) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level A Disintegrin And Metalloprotease 17 (ADAM17) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level A Disintegrin And Metalloprotease 17 (ADAM17) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:ADAM17) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

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Magazine Citations
Int Wound J. From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases. Pubmed:26991748
International Wound Journal From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases pubmed:26991748
Biomedical Research ADAM17 regulates TNF-α expression upon lipopolysaccharide stimulation in oral keratinocytes. pubmed:28637950
Cytometry B Clin Cytom L‐Selectin Expression Is Influenced by Phosphatase Activity in Chronic Lymphocytic Leukemia Pubmed: 30729673
Disease Markers Adamalysines as Biomarkers and a Potential Target of Therapy in Colorectal Cancer Patients: Preliminary Results Pubmed: 31565100
Chem Biol Interact PEG-SOD attenuates the mitogenic ERK1/2 signaling cascade induced by cyclosporin A in the liver and kidney of albino mice Pubmed: 32866465
life High Levels of TNF-¦Á and TIM-3 as a Biomarker of Immune Reconstitution Inflammatory Syndrome in People with HIV Infection
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
SEB555Mu ELISA Kit for A Disintegrin And Metalloprotease 17 (ADAM17) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB555Mu Multiplex Assay Kit for A Disintegrin And Metalloprotease 17 (ADAM17) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.