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Multiplex Assay Kit for Tumor Necrosis Factor Ligand Superfamily, Member 13 (TNFSF13) ,etc. by FLIA (Flow Luminescence Immunoassay)

CD256; APRIL; TALL2; TRDL1; ZTNF2; A Proliferation Inducing Ligand; TNF And APOL Related Leukocyte Expressed Ligand 2; TNF-Related Death Ligand 1

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Tumor Necrosis Factor Ligand Superfamily, Member 13 (TNFSF13) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Tumor Necrosis Factor Ligand Superfamily, Member 13 (TNFSF13) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tumor Necrosis Factor Ligand Superfamily, Member 13 (TNFSF13) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tumor Necrosis Factor Ligand Superfamily, Member 13 (TNFSF13) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:TNFSF13) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

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Magazine Citations
Journal of Critical Care Serum concentrations of A Proliferation-Inducing Ligand (APRIL) are elevated in sepsis and predict mortality in critically ill patients PubMed: 23337484
Haematologica Arrayed molecular barcoding identifies TNFSF13 as a positive regulator of acute myeloid leukemia-initiating cells Pubmed: 30819903
Gene A Significant Decrease of BAFF, APRIL, and BAFF Receptors Following Mesenchymal Stem Cell Transplantation in Patients with Refractory Rheumatoid Arthritis Pubmed: 31935514
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPB750Ra01 Recombinant Tumor Necrosis Factor Ligand Superfamily, Member 13 (TNFSF13) Positive Control; Immunogen; SDS-PAGE; WB.
PAB750Ra01 Polyclonal Antibody to Tumor Necrosis Factor Ligand Superfamily, Member 13 (TNFSF13) WB; IHC; ICC; IP.
MAB750Ra21 Monoclonal Antibody to Tumor Necrosis Factor Ligand Superfamily, Member 13 (TNFSF13) WB; IHC; ICC; IP.
SEB750Ra ELISA Kit for Tumor Necrosis Factor Ligand Superfamily, Member 13 (TNFSF13) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB750Ra Multiplex Assay Kit for Tumor Necrosis Factor Ligand Superfamily, Member 13 (TNFSF13) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.