≡

Multiplex Assay Kit for Vitamin B1 (VB1) ,etc. by FLIA (Flow Luminescence Immunoassay)

Thiamine; Thiamin; Aneurine

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Vitamin B1 (VB1) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Vitamin B1 (VB1) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of Vitamin B1 (VB1) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Vitamin B1 (VB1) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 98-105 101
EDTA plasma(n=5) 97-105 101
heparin plasma(n=5) 78-97 84

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vitamin B1 (VB1) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vitamin B1 (VB1) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Vitamin B1 (VB1) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 99-105% 89-96% 93-101% 80-96%
EDTA plasma(n=5) 98-105% 88-104% 90-97% 95-102%
heparin plasma(n=5) 91-101% 79-101% 97-104% 91-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:VB1) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
    add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Genes and Immunity Identification of critical variants within SLC44A4, an ulcerative colitis susceptibility gene identified in a GWAS in north Indians Pubmed:26741288
畜牧兽医学报 日粮不同钴水平对奶牛泌乳性能, 营养物质消化率和血液生化指标的影响
Biomedicines Disinhibition-Like Behavior Correlates with Frontal Cortex Damage in an Animal Model of Chronic Alcohol Consumption and Thiamine Deficiency Pubmed:35203470
Catalog No. Related products for research use of Pan-species (General) Organism species Applications (RESEARCH USE ONLY!)
CPD053Ge11 BSA Conjugated Vitamin B1 (VB1) Immunogen; SDS-PAGE; WB.
CPD053Ge21 OVA Conjugated Vitamin B1 (VB1) Immunogen; SDS-PAGE; WB.
PAD053Ge01 Polyclonal Antibody to Vitamin B1 (VB1) ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
CED053Ge ELISA Kit for Vitamin B1 (VB1) Enzyme-linked immunosorbent assay for Antigen Detection.
CCD053Ge CLIA Kit for Vitamin B1 (VB1) Chemiluminescent immunoassay for Antigen Detection.
LMD053Ge Multiplex Assay Kit for Vitamin B1 (VB1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSD053Ge11 ELISA Kit DIY Materials for Vitamin B1 (VB1) Main materials for "Do It (ELISA Kit) Yourself".