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Multiplex Assay Kit for Adropin (AD) ,etc. by FLIA (Flow Luminescence Immunoassay)

ENHO; UNQ470; Energy Homeostasis Associated Protein

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Adropin (AD) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Adropin (AD) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Adropin (AD) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Adropin (AD) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 99-105 102
EDTA plasma(n=5) 97-105 101
heparin plasma(n=5) 78-95 82
sodium citrate plasma(n=5) 79-103 81

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adropin (AD) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adropin (AD) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Adropin (AD) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 93-101% 89-103% 97-104% 79-102%
EDTA plasma(n=5) 94-101% 94-101% 92-104% 82-92%
heparin plasma(n=5) 89-103% 98-105% 95-102% 95-103%
sodium citrate plasma(n=5) 80-93% 78-90% 82-103% 86-103%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:AD) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

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Magazine Citations
Pregnancy Hypertens. Alteration of serum adropin level in preeclampsia. pubmed:28501281
Frontiers in Molecular Neuroscience Adropin Is a Key Mediator of Hypoxia Induced Anti-Dipsogenic Effects via TRPV4-CamKK-AMPK Signaling in the Circumventricular Organs of Rats. pubmed:28473751
Preprints Afamin and Adropin in Patients with Alcohol-Induced Liver Cirrhosis manuscript:201803.0258
Probiotics and Antimicrobial Proteins  Effects of Probiotic Yogurt on Serum Omentin-1, Adropin, and Nesfatin-1 Concentrations in Overweight and Obese Participants Under Low-Calorie Diet Pubmed: 30232744
Probiotics and Antimicrobial Proteins Mitra Zarrati, Mahsa Raji Lahiji, Eisa Salehi, Bahareh Yazdani, Elham Razmpoosh, Raheleh Shokouhi Shoormasti & Farzad Shidfar Pubmed: 30232744
Pediatr Diabetes Adropin and apelin‐12 efficiently predict metabolic syndrome in obese children Pubmed: 32749012
European Journal of Therapeutics Effect of Peri-Implant Disease on Adropin Levels: A Cross-Sectional Pilot Study
Horm Mol Biol Clin Investig Effects of eight weeks exercise training on serum levels of adropin in male volleyball players 33794077
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPN251Mu01 Recombinant Adropin (AD) Positive Control; Immunogen; SDS-PAGE; WB.
PAN251Mu01 Polyclonal Antibody to Adropin (AD) WB; IHC; ICC; IP.
SEN251Mu ELISA Kit for Adropin (AD) Enzyme-linked immunosorbent assay for Antigen Detection.
LMN251Mu Multiplex Assay Kit for Adropin (AD) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.