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Image (II)
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Western Blot; Sample: Lane1: Human Placenta lysate; Lane2: U2OS cell lysate; Lane3: Porcine Liver lysate; Lane4: Porcine Cerebrum lysate Primary Ab: 0.2μg/ml Mouse Anti-Multi-species H3 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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Western Blot; Samples: Lane1: 293F cell lysate; Lane2: K562 cell lysate; Lane3: Jurkat cell lysate; Lane4: Porcine Cerebrum lysate;
Primary Ab: 0.2µg/ml Mouse Anti-Multi-species H3 Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
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Instruction Manual
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Monoclonal Antibody to Histone H3 (H3)
- Product No.MAA285Mi24
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourceMonoclonal antibody preparation
- HostMouse
- Potency (Clone Number)C7
- Ig Isotype IgG1 Kappa
- PurificationProtein A + Protein G affinity chromatography
- LabelNone
- Immunogen RPA285Mi01-Recombinant Histone H3 (H3)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration1mg/mL
- Organism Species MoreSus scrofa; Porcine (Pig)
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies. - DownloadInstruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
- FOB
US$ 114
US$ 266
US$ 380
US$ 950
US$ 3800
For more details, please contact local distributors!
SPECIFITY
The antibody is a mouse monoclonal antibody raised against H3. It has been selected for its ability to recognize H3 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.5-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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| Magazine | Citations |
| Biomed Res Int | A Novel Anti-Histone H1 Monoclonal Antibody, SSV Monoclonal Antibody, Improves Lung Injury and Survival in a Mouse Model of Lipopolysaccharide-Induced Sepsis-Like Syndrome PubMed: 25649890 |
| Cell Death & Disease | Proteomic analysis reveals USP7 as a novel regulator of palmitic acid-induced hepatocellular carcinoma cell death Pubmed:35732625 |
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| RPA285Mi01 | Recombinant Histone H3 (H3) | Positive Control; Immunogen; SDS-PAGE; WB. |
| CAA285Mi01 | Anti-Histone H3 (H3) Polyclonal Antibody | Loading Control of WB. |
| PAA285Mi01 | Polyclonal Antibody to Histone H3 (H3) | WB; IHC; ICC; IP. |
| MAA285Mi21 | Monoclonal Antibody to Histone H3 (H3) | WB; IHC; ICC; IP. |
| MAA285Mi22 | Monoclonal Antibody to Histone H3 (H3) | WB |
| MAA285Mi23 | Monoclonal Antibody to Histone H3 (H3) | WB; IHC; ICC; IP. |
| MAA285Mi24 | Monoclonal Antibody to Histone H3 (H3) | WB; IHC; ICC; IP. |
| SEA285Mi | ELISA Kit for Histone H3 (H3) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA285Mi | Multiplex Assay Kit for Histone H3 (H3) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |