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Polyclonal Antibody to Histone H3 (H3)

  • Product No.PAA285Mi01
  • Organism SpeciesHomo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat), Oryctolagus cuniculus (Rabbit), Rhesus monkey (Simian), Canis familiaris; Canine (Dog), Bos taurus; Bovine (Cattle), Equus caballus; Equine (Horse), Chicken (Gallus) Same name, Different species.
  • SourcePolyclonal antibody preparation
  • HostRabbit
  • Potencyn/a
  • Ig Type IgG
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPA285Mi01-Recombinant Histone H3 (H3)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid
  • Concentration0.5mg/ml
  • Organism Species MoreHomo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat), Cavia (Guinea pig ), Rhesus monkey (Simian), Canis familiaris; Canine (Dog), Sus scrofa; Porcine (Pig), Bos taurus; Bovine (Cattle), Capra hircus; Caprine (Goat), Ovis aries; Ovine (Sheep), Equus caballus; Equine (Horse), Chicken (Gallus)
  • ApplicationsWB; IHC; ICC; IP.
    If the antibody is used in flow cytometry, please check FCM antibodies.
  • DownloadInstruction Manual
  • UOM 20µl100µl 200µl 1ml 10ml
  • FOB US$ 110  US$ 258  US$ 368  US$ 920  US$ 3680 
    For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against H3. It has been selected for its ability to recognize H3 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Biomed Res Int A Novel Anti-Histone H1 Monoclonal Antibody, SSV Monoclonal Antibody, Improves Lung Injury and Survival in a Mouse Model of Lipopolysaccharide-Induced Sepsis-Like Syndrome PubMed: 25649890
Cell Death & Disease Proteomic analysis reveals USP7 as a novel regulator of palmitic acid-induced hepatocellular carcinoma cell death Pubmed:35732625
Catalog No. Related products for research use of Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat), Oryctolagus cuniculus (Rabbit), Rhesus monkey (Simian), Canis familiaris; Canine (Dog), Bos taurus; Bovine (Cattle), Equus caballus; Equine (Horse), Chicken (Gallus) Organism species Applications (RESEARCH USE ONLY!)
RPA285Mi01 Recombinant Histone H3 (H3) Positive Control; Immunogen; SDS-PAGE; WB.
CAA285Mi01 Anti-Histone H3 (H3) Polyclonal Antibody Loading Control of WB.
PAA285Mi01 Polyclonal Antibody to Histone H3 (H3) WB; IHC; ICC; IP.
MAA285Mi21 Monoclonal Antibody to Histone H3 (H3) WB; IHC; ICC; IP.
MAA285Mi22 Monoclonal Antibody to Histone H3 (H3) WB
MAA285Mi23 Monoclonal Antibody to Histone H3 (H3) WB; IHC; ICC; IP.
MAA285Mi24 Monoclonal Antibody to Histone H3 (H3) WB; IHC; ICC; IP.
SEA285Mi ELISA Kit for Histone H3 (H3) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA285Mi Multiplex Assay Kit for Histone H3 (H3) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.