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Mini Samples ELISA Kit for Interleukin 8 (IL8)
CXCL8; AMCF-I; GCP1; K60; LECT; LUCT; LYNAP; MDNCF; MONAP; NAF; NAP1; SCYB8; TSG1; B-ENAP; Neutrophil-Activating Protein 1; Granulocyte Chemotactic Protein 1
- Product No.MEA080Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range0.156-10ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.063ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
- FOB
US$ 385
US$ 550
US$ 2475
US$ 4675
US$ 38500
For more details, please contact local distributors!
Specificity
This assay has high sensitivity and excellent specificity for detection of Mini Samples Interleukin 8 (IL8).
No significant cross-reactivity or interference between Mini Samples Interleukin 8 (IL8) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Mini Samples Interleukin 8 (IL8) and the recovery rates were calculated by comparing the measured value to the expected amount of Mini Samples Interleukin 8 (IL8) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 97-105 | 101 |
| EDTA plasma(n=5) | 93-103 | 98 |
| heparin plasma(n=5) | 87-101 | 92 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Interleukin 8 (IL8) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Interleukin 8 (IL8) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mini Samples Interleukin 8 (IL8) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 86-101% | 88-96% | 92-102% | 79-95% |
| EDTA plasma(n=5) | 80-96% | 91-101% | 99-105% | 96-104% |
| heparin plasma(n=5) | 83-103% | 99-105% | 80-92% | 87-97% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×60µL | Assay Diluent A | 1×6mL |
| Detection Reagent B | 1×60µL | Assay Diluent B | 1×6mL |
| TMB Substrate | 1×4.5mL | Stop Solution | 1×3mL |
| Wash Buffer (30 × concentrate) | 1×10mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 25µL standard or sample to each well. Incubate 1 hour at 37°C;
3. Aspirate and add 25µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 25µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 25µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 20µL Stop Solution. Read at 450nm immediately.
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| Magazine | Citations |
| Reproductive Biology and Endocrinology | Evidence that polymorphonuclear neutrophils infiltrate into the developing corpus luteum and promote angiogenesis with interleukin-8 in the cow Rbej: 14777827 |
| Acta Otolaryngol | Serial cytokine levels during wound healing in rabbit maxillary sinus mucosa PubMed: 19958244 |
| Journal of Clinical Periodontology | Experimental peri-implant mucositis at different implant surfaces. Pubmed: 24521508 |
| Physiological Research | Budesonide added to modified porcine surfactant Curosurf may additionally improve the lung functions in meconium aspiration syndrome. Pubmed: 2432969 |
| Rheumatology International | Serum levels of selected chemokines in systemic lupus erythematosus patients Pubmed: 22461186 |
| J Med Life. | Expression of interleukine-8 as an independent prognostic factor for sporadic colon cancer dissemination Pubmed:Pmc4197484 |
| J Clin Periodontol. | Experimental peri‐implant mucositis at different implant surfaces Pubmed:24521508 |
| British Journal of Nutrition | Grape seed extract supplementation attenuates the heat stress-induced responses of jejunum epithelial cells in Simmental?×?Qinchuan steers Pubmed:24846452 |
| Theriogenology | Inflammatory cytokine concentrations in uterine flush and serum samples from dairy cows with clinical or subclinical endometritis Theriojournal:Source |
| Anim Reprod Sci. | Alteration in peripheral blood concentration of certain pro-inflammatory cytokines in cows developing retention of fetal membranes PubMed: 25851495 |
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| Archives of Biological Sciences | Diabetes-induced renal failure is associated with tissue inflammation and neutrophil gelatinase-associated lipocalin: Effects of resveratrol publication:299492265_Diabetes-induced_renal_failu |
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| PLOS ONE | Mechanical Ventilation Alters the Development of Staphylococcus aureus Pneumonia in Rabbit. pubmed:27391952 |
| The Journal of Comenius University in Bratislava | Effects of Conventional Mechanical Ventilation Performed by Two Neonatal Ventilators on the Lung Functions of Rabbits with Meconium-Induced Acute Lung Injury acm.2017.16.issue-3 |
| Journal of Physiology and Pharmacology | Effects of budesonide on the lung functions, inflammation and apoptosis in a saline-lavagemodel of acute lung injury. pubmed:28195073 |
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| Veterinary Immunology and Immunopathology | Cytokine levels in colostrum and in foals' serum pre- and post-suckling pubmed:28242000 |
| Journal of Cellular Physiology | Increased Chondrogenic Potential of Mesenchymal Cells From Adipose Tissue Versus Bone Marrow‐Derived Cells in Osteoarthritic In Vitro Models pubmed:27739057 |
| European Journal of Pharmacology | Cardioprotective time-window of Penehyclidine hydrochloride postconditioning: A rat study pubmed:28684235 |
| PLoS One | Linezolid and atorvastatin impact on pneumonia caused by Staphyloccocus aureus in rabbits with or without mechanical ventilation pubmed:29149185 |
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| Experimentelle periimplantäre Mukositis an unterschiedlichen Implantatoberflächen doi: 10.1111/jcpe.12240. | |
| Molecular Medicine Reports | CagA promotes proliferation and inhibits apoptosis of GES-1 cells by upregulating TRAF1/4-1BB pubmed:28627614 |
| ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY | Dexamethasone reduces serum level of IL-17 in Bleomycin-A5-induced rats model of pulmonary fibrosis pubmed:28608724 |
| Biological Rhythm Research | Alteration in some pro and anti-inflammatory cytokines associated with complete and incomplete gestation cycle of cows 10.1080/09291016.2017.1319636 |
| Scientific Reports | Optimization of Storage Temperature for Retention of Undifferentiated Cell Character of Cultured Human Epidermal Cell Sheets pubmed:28811665 |
| Journal of Ultrasound in Medicine | Inhibitory Effect of Low-Intensity Pulsed Ultrasound on the Expression of Lipopolysaccharide-Induced Inflammatory Factors in U937 Cells. pubmed:28600899 |
| Veterinary Immunology and Immunopathology | Integrated effect of seasons and lactation stages on the plasma inflammatory cytokines, function and receptor expression of milk neutrophils in Sahiwal (Bos indicus) cows. pubmed:28895861 |
| Biomedicine hub | Comparison of Polymyxin E and Polymyxin B as an Additive to Pulmonary Surfactant in Escherichia coli Pneumonia of Ventilated Neonatal Rabbits 10.1159/000475877 |
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| Life Sciences | Dexmedetomidine attenuates renal fibrosis via α2-adrenergic receptor-dependent inhibition of cellular senescence after renal ischemia/reperfusion Pubmed:29729264 |
| Microbial Pathogenesis | Pathogen-dependent modulation of milk neutrophils competence, plasma inflammatory cytokines and milk quality during intramammary infection of Sahiwal (Bos … Pubmed:29787791 |
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| Journal of Veterinary Science | Rifaximin anti‐inflammatory activity on bovine endometrium primary cell cultures: a preliminary study Pubmed:29984902 |
| Bioscience Reports | N-Acetylcysteine inhalation improves pulmonary function in patients received Liver Transplantation. Doi: 10.1042/BSR20180858 |
| Acta Medica Mediterranea | Effects of Conventional Mechanical Ventilation Performed by Two Neonatal Ventilators on the Lung Functions of Rabbits with Meconium-Induced Acute Lung … Doi: 10.1515/acm-2016-0012 |
| Journal of Dairy Science | Metabolism and immune status during transition period influences the lactation performance in Zebu (Bos indicus () cows Pubmed: 17430930 |
| Biomedicine & Pharmacotherapy | Interleukin 8 targeted contrast echocardiography is effective to evaluate myocardial ischemia-reperfusion injury in the rabbits |
| Emerging Microbes & Infections | Immunogenicity and protective efficacy against Treponema pallidum in New Zealand rabbits immunized with plasmid DNA encoding flagellin Pubmed: 30405111 |
| Journal of Investigative Medicine | The characteristics and clinical significance of mucin levels in bronchoalveolar lavage fluid of patients with interstitial lung disease Pubmed: 30573494 |
| International Journal of Chronic Obstructive Pulmonary Disease | Altered serum levels of type I collagen turnover indicators accompanied by IL-6 and IL-8 release in stable COPD Pubmed: 30655663 |
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| BioMed Research International | Effective Treatment of Bovine Mastitis with Intramammary Infusion of Angelica dahurica and Rheum officinale Extracts |
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| Scientific Reports | Corrosion Resistance of Graphene oxide/Silver Coatings on Ni–Ti alloy and Expression of IL-6 and IL-8 in Human Oral Fibroblasts Pubmed: 32094428 |
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| Antibiotics | Effects of Early Intervention with Antibiotics and Maternal Fecal Microbiota on Transcriptomic Profiling Ileal Mucusa in Neonatal Pigs Pubmed: 31963653 |
| Physiol Res | Effect of Different Dosages of Dexamethasone Therapy on Lung Function and Inflammation in an Early Phase of Acute Respiratory Distress Syndrome Model Pubmed: 31928043 |
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| INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE | Possible Anti-inflammatory Effects of Mesenchymal Stem Cells Transplantation via Changes in CXCL8 Levels in Patients with Refractory Rheumatoid Arthritis |
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| J Med Food | Cough Inhibition Activity of Schisandra chinensis in Guinea Pigs 33861937 |
| Sci Rep | Association between MIF gene promoter rs755622 and susceptibility to coronary artery disease and inflammatory cytokines in the Chinese Han population 33850223 |
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| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| APA080Hu01 | Active Interleukin 8 (IL8) | Cell culture; Activity Assays. |
| RPA080Hu01 | Recombinant Interleukin 8 (IL8) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA080Hu01 | Polyclonal Antibody to Interleukin 8 (IL8) | WB; IHC; ICC; IP. |
| LAA080Hu71 | Biotin-Linked Polyclonal Antibody to Interleukin 8 (IL8) | WB; IHC; ICC. |
| LAA080Hu81 | FITC-Linked Polyclonal Antibody to Interleukin 8 (IL8) | WB; IHC; ICC; IF. |
| MAA080Hu24 | Monoclonal Antibody to Interleukin 8 (IL8) | WB; IHC; ICC; IP. |
| MAA080Hu21 | Monoclonal Antibody to Interleukin 8 (IL8) | WB; IHC; ICC; IP. |
| MAA080Hu22 | Monoclonal Antibody to Interleukin 8 (IL8) | WB; IHC; ICC; IP. |
| LAA080Hu82 | FITC-Linked Monoclonal Antibody to Interleukin 8 (IL8) | WB; IHC; ICC; IF. |
| LAA080Hu72 | Biotin-Linked Monoclonal Antibody to Interleukin 8 (IL8) | WB; IHC; ICC. |
| FAA080Hu22 | Labelled Anti-Interleukin 8 (IL8) Monoclonal Antibody | FCM |
| MEA080Hu | Mini Samples ELISA Kit for Interleukin 8 (IL8) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEA080Hu | ELISA Kit for Interleukin 8 (IL8) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| HEA080Hu | High Sensitive ELISA Kit for Interleukin 8 (IL8) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| IEA080Hu | Instant ELISA Kit for Interleukin 8 (IL8) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| AEA080Hu | ELISA Kit for Anti-Interleukin 8 Antibody (Anti-IL8) | Enzyme-linked immunosorbent assay for Antibody Detection. |
| SCA080Hu | CLIA Kit for Interleukin 8 (IL8) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA080Hu | Multiplex Assay Kit for Interleukin 8 (IL8) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
| PSA080Hu01 | Antibody Pair for Interleukin 8 (IL8) | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| KSA080Hu01 | ELISA Kit DIY Materials for Interleukin 8 (IL8) | Main materials for "Do It (ELISA Kit) Yourself". |