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Mini Samples ELISA Kit for Interleukin 27A (IL27A)
p28; IL30; Interleukin 30; Interleukin-27 subunit alpha; IL-27 subunit alpha; IL27-A
- Product No.MEA385Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range15.6-1,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 6.4pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
- FOB
US$ 575
US$ 821
US$ 3695
US$ 6979
US$ 57470
For more details, please contact local distributors!
Specificity
This assay has high sensitivity and excellent specificity for detection of Mini Samples Interleukin 27A (IL27A).
No significant cross-reactivity or interference between Mini Samples Interleukin 27A (IL27A) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Mini Samples Interleukin 27A (IL27A) and the recovery rates were calculated by comparing the measured value to the expected amount of Mini Samples Interleukin 27A (IL27A) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 82-99 | 95 |
| EDTA plasma(n=5) | 79-89 | 79 |
| heparin plasma(n=5) | 94-102 | 97 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Interleukin 27A (IL27A) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Interleukin 27A (IL27A) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mini Samples Interleukin 27A (IL27A) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 81-97% | 97-105% | 79-104% | 88-101% |
| EDTA plasma(n=5) | 81-94% | 89-103% | 79-101% | 89-97% |
| heparin plasma(n=5) | 88-104% | 78-101% | 91-102% | 82-90% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×60µL | Assay Diluent A | 1×6mL |
| Detection Reagent B | 1×60µL | Assay Diluent B | 1×6mL |
| TMB Substrate | 1×4.5mL | Stop Solution | 1×3mL |
| Wash Buffer (30 × concentrate) | 1×10mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 25µL standard or sample to each well. Incubate 1 hour at 37°C;
3. Aspirate and add 25µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 25µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 25µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 20µL Stop Solution. Read at 450nm immediately.
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| Magazine | Citations |
| Human Immunology | Elevated interleukin-27 enhances the polarization of Th1/Tc1 cells and the production of proinflammatory cytokines in primary immune thrombocytopenia Pubmed: 22210084 |
| Front Pharmacol | Poria Attenuates Idiosyncratic Liver Injury Induced by Polygoni Multiflori Radix Praeparata pubmed:27803670 |
| cyberleninka | КЛИНИКО-ЭПИДЕМИОЛОГИЧЕСКАЯ ХАРАКТЕРИСТИКА И ОСОБЕННОСТИ БАЛЛАНСА ЦИТОКИНОВ ПРИ КЛЕЩЕВОМ ЭНЦЕФАЛИТЕ В … |
| Int J Lab Hematol | Interlukin‐27 rs153109 polymorphism confers the susceptibility and prognosis of aplastic anemia in Chinese population 34528397 |
| Catalog No. | Related products for research use of Mus musculus (Mouse) Organism species | Applications (RESEARCH USE ONLY!) |
| EPA385Mu61 | Eukaryotic Interleukin 27A (IL27A) | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA385Mu61 | Active Interleukin 27A (IL27A) | Cell culture; Activity Assays. |
| RPA385Mu01 | Recombinant Interleukin 27A (IL27A) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA385Mu01 | Polyclonal Antibody to Interleukin 27A (IL27A) | WB; IHC; ICC; IP. |
| SEA385Mu | ELISA Kit for Interleukin 27A (IL27A) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEA385Mu | Mini Samples ELISA Kit for Interleukin 27A (IL27A) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA385Mu | Multiplex Assay Kit for Interleukin 27A (IL27A) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |