Packages (Simulation)
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Western Blot; Sample: Porcine Spleen lysate; Primary Ab: 1µg/ml Rabbit Anti-Porcine H4 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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Western Blot; Sample: Mouse Thymus lysate;
Primary Ab: 1µg/ml Rabbit Anti-Porcine H4 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Mouse Placenta lysate;
Primary Ab: 1µg/ml Rabbit Anti-Porcine H4 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Human Hela cell lysate;
Primary Ab: 1µg/ml Rabbit Anti-Porcine H4 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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Instruction Manual
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Polyclonal Antibody to Histone H4 (H4)
- Product No.PAA289Po01
- Organism SpeciesSus scrofa; Porcine (Pig) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen n/a
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid
- Concentration0.33mg/mL
- Organism Species MoreHomo sapiens (Human), Mus musculus (Mouse)
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies. - DownloadInstruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
- FOB
US$ 114
US$ 266
US$ 380
US$ 950
US$ 3800
For more details, please contact local distributors!
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against H4. It has been selected for its ability to recognize H4 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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| Magazine | Citations |
| PLoS One. | N-Acetyl-Heparin Attenuates Acute Lung Injury Caused by Acid Aspiration Mainly by Antagonizing Histones in Mice Pubmed:24816808 |
| Crit Care. | Impact of plasma histones in human sepsis and their contribution to cellular injury and inflammation Pubmed:25260379 |
| Biomed Res Int | A Novel Anti-Histone H2 Monoclonal Antibody, SSV Monoclonal Antibody, Improves Lung Injury and Survival in a Mouse Model of Lipopolysaccharide-Induced Sepsis-Like Syndrome PubMed: 25649891 |
| Journal of Viral Hepatitis | Patients with HBV-related acute-on-chronic liver failure have increased concentrations of extracellular histones aggravating cellular damage and systemic inflammation pubmed:27660136 |
| Cell Death & Disease | Dexmedetomidine inhibits astrocyte pyroptosis and subsequently protects the brain in in vitro and in vivo models of sepsis Pubmed: 30778043 |
| Respir Res | Histone H4 induces heparan sulfate degradation by activating heparanase in chlorine gas-induced acute respiratory distress syndrome Pubmed:35073921 |
| Catalog No. | Related products for research use of Sus scrofa; Porcine (Pig) Organism species | Applications (RESEARCH USE ONLY!) |
| PAA289Po01 | Polyclonal Antibody to Histone H4 (H4) | WB; IHC; ICC; IP. |