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Recombinant Collagen Type IV Alpha 1 (COL4a1)

COL4-A1; Arresten; Collagen Alpha-1(IV)chain

  • Product No.RPA149Ra01
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • SourceProkaryotic expression
  • HostE.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationSecreted
  • Predicted Molecular Mass31.3kDa
  • Accurate Molecular Mass31kDa(Analysis of differences refer to the manual)
  • Residues & TagsPro1416~Thr1669 with N-terminal His Tag
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder
  • Purity> 95%
  • Isoelectric Point7.8
  • Applications Positive Control; Immunogen; SDS-PAGE; WB.
    If bio-activity of the protein is needed, please check active protein.
  • DownloadInstruction Manual
  • UOM 10µg50µg 200µg 1mg 5mg
  • FOB US$ 140  US$ 350  US$ 700  US$ 2100  US$ 5250 
    For more details, please contact local distributors!

SEQUENCE

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Cancer Research Peritoneal Dissemination Requires an Sp1-Dependent CXCR4/CXCL12 Signaling Axis and Extracellular Matrix–Directed Spheroid Formation Pubmed:26744523
PLoS One miR-146a regulates glucose induced upregulation of inflammatory cytokines extracellular matrix proteins in the retina and kidney in diabetes 10.1371
Journal of Diabetes & Metabolic Disorders Endothelin-1 traps potently reduce pathologic markers back to basal levels in an in vitro model of diabetes
Experimental and Molecular Pathology  Epitope recognized by anti-glomerular basement membrane (GBM) antibody in a patient with repeated relapse of anti-GBM disease Pubmed: 30817909
Journal of Diabetes & Metabolic Disorders In vivo studies demonstrate that endothelin-1 traps are a potential therapy for type I diabetes
Frontiers in Endocrinology Curcumin Analogs Reduce Stress and Inflammation Indices in Experimental Models of Diabetes Pubmed: 31920992
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPA149Ra01 Recombinant Collagen Type IV Alpha 1 (COL4a1) Positive Control; Immunogen; SDS-PAGE; WB.
PAA149Ra01 Polyclonal Antibody to Collagen Type IV Alpha 1 (COL4a1) WB; IHC; ICC; IP.
MAA149Ra21 Monoclonal Antibody to Collagen Type IV Alpha 1 (COL4a1) WB; IHC; ICC; IP.
SEA149Ra ELISA Kit for Collagen Type IV Alpha 1 (COL4a1) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA149Ra Multiplex Assay Kit for Collagen Type IV Alpha 1 (COL4a1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.