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Recombinant Interferon Beta (IFNb)

IFNB1; IFN-B; IFB; IFF; IFNB; Interferon Beta 1 Fibroblast

  • Product No.RPA222Ra02
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • SourceProkaryotic expression
  • HostE.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationSecreted
  • Predicted Molecular Mass21.4kDa
  • Accurate Molecular Mass22kDa(Analysis of differences refer to the manual)
  • Residues & TagsAsp23~Phe169 with N-terminal His Tag
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder
  • Purity> 95%
  • Isoelectric Point9.7
  • Applications Positive Control; Immunogen; SDS-PAGE; WB.
    If bio-activity of the protein is needed, please check active protein.
  • DownloadInstruction Manual
  • UOM 10µg50µg 200µg 1mg 5mg
  • FOB US$ 174  US$ 435  US$ 870  US$ 2610  US$ 6525 
    For more details, please contact local distributors!

SEQUENCE

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
20 Melatonin protects liver against ischemia and reperfusion injury through inhibition of toll-like receptor signaling pathway PubMed: 21355876
Journal of General Virology Hepatitis B virus polymerase inhibits RIG-I- and Toll-like receptor 3-mediated beta interferon induction in human hepatocytes through interference with interferon regulatory factor 3 activation and dampening of the interaction between TBK1/IKKε and DDX3. Sgm: 9182080
Biochemical and Biophysical Research Communications Impaired TLR3/IFN-β signaling in monocyte-derived dendritic cells from patients with acute-on-chronic hepatitis B liver failure: Relevance to the severity of liver damage ScienceDirect: S0006291X09019822
PLoS One Intact Type I Interferon Production and IRF7 Function in Sooty Mangabeys PubMed: PMC3757038
PLoS Neglected Tropical Diseases Innate Immune Response to Rift Valley Fever Virus in Goats PubMed: PMC3335883
BioMed Research International Low pH Environmental Stress Inhibits LPS and LTA-Stimulated Proinflammatory Cytokine Production in Rat Alveolar Macrophages Pubmed: 24288685
Vet Microbiol. An attenuated EIAV strain and its molecular clone strain differentially induce the expression of Toll-like receptors and type-I interferons in equine monocyte-derived macrophages. Pubmed: 23850441
Food and Chemical Toxicology Scoparone attenuates d-galactosamine/lipopolysaccharide-induced fulminant hepatic failure through inhibition of toll-like receptor 4 signaling in mice Pubmed: 23535186
Veterinary immunology and immunopathology Experimental model of equine alveolar macrophage stimulation with TLR ligands Pubmed: 23815824
American journal of respiratory cell and molecular biology Training Modifies Innate Immune Responses in Blood Monocytes and in Pulmonary Alveolar Macrophages Pubmed: 24502337
FEBS Lett. Mannan binding lectin attenuates double-stranded RNA-mediated TLR3 activation and innate immunity Pubmed:24530528
Veterinary Research Infection of equine monocyte-derived macrophages with an attenuated equine infectious anemia virus (EIAV) strain induces a strong resistance to the infection by a virulent EIAV strain Pubmed:25106750
PLOS Neglected Tropical Diseases MicroRNA-30e* Suppresses Dengue Virus Replication by Promoting NF-κB–Dependent IFN Production Pubmed:25122182
Veterinary Research The innate immune response of equine bronchial epithelial cells is altered by training Pubmed:25595212
molecular and celluar biology SOCS3 Drives Proteasomal Degradation of TBK1 and Negatively Regulates Antiviral Innate Immunity PubMed: 25939384
Arch Virol Analysis of cytokine production in a newly developed canine tracheal epithelial cell line infected with H3N3 canine influenza virus PubMed: 25797196
J Complexes of DNA with the Antimicrobial Peptide LL37 Augment NK Cell Functions by Inducing Type I Interferon Production from Circulating Monocytes and Plasmacytoid Predendritic Cells PubMed: 26258404
Virus PKR activation enhances replication of classical swine fever virus in PK-15 cells PubMed: 25899421
PLoS One siRNA Targeting the 2A pro Genomic Region Prevents Enterovirus 71 Replication In Vitro Pubmed:26886455
Scientific Reports Cellular uptake of exogenous calcineurin B is dependent on TLR4/MD2/CD14 complexes, and CnB is an endogenous ligand of TLR4 Pubmed:27090571
Front Microbiol Innate Immune Responses in ALV-J Infected Chicks and Chickens with Hemangioma In Vivo Pubmed:27252695
Canadian Journal of Physiology and Pharmacology Chloroquine attenuates lipopolysaccharide-induced inflammatory responses through upregulation of USP25. pubmed:28134560
International Journal of Molecular Medicine Effects of IRF1 and IFN-β interaction on the M1 polarization of macrophages and its antitumor function pubmed:27176664
International Journal of Clinical and Experimental Pathology Host miR-146a promotes replication of human cytomegalovirus by suppressing type I IFN response in MRC-5 cells files:ijcep0036479.pdf
Poultry Science ALV-J strain SCAU-HN06 induces innate immune responses in chicken primary monocyte-derived macrophages pubmed:27486255
Journal of Virology Characterization of Simian Immunodeficiency Virus Variants Anatomically Compartmentalized inPlasma and Milk in Chronically Infected African Green Monkeys. pubmed:
Biochemical and Biophysical Research Communications MicroRNA-548j inhibits type I interferon production by targeting ZBTB11 in patients with chronic hepatitis B pubmed:28342861
Virology The down-regulation of casein kinase 1 alpha as a host defense response against infectious bursal disease virus infection pubmed:28988058
EMBO Journal Zika virus elicits inflammation to evade antiviral response by cleaving cGAS via NS1‐caspase‐1 axis Pubmed:30065070
Toxicology and applied pharmacology Bisphenol A induced male germ cell apoptosis via IFNβ-XAF1-XIAP pathway in adult mice Pubmed:30017639
Archives of Virology MAVS induces a host cell defense to inhibit CSFV infection Pubmed:29556776
Journal of virology Inhibition of DNA-sensing pathway by Marek's disease virus VP23 protein through suppression of interferon regulatory factor 7 activation Pubmed: 30518647
Veterinary Immunology and Immunopathology Molecular cloning and functional characterization of porcine 2′, 5′-oligoadenylate synthetase 1b and its effect on infection with porcine reproductive and respiratory?… Doi: 10.1016/j.vetimm.2019.01.003
Science Signaling HIPK2 is necessary for type I interferon–mediated antiviral immunity Doi: 10.1126/scisignal.aau4604
INTERNATIONAL MEDICAL RESEARCH Evaluation of serum interferons in patients with age-related macular degeneration Pubmed: 31007694
Veterinary Immunology and Immunopathology Differential expression of type I interferon mRNA and protein levels induced by virulent Marek's disease virus infection in chickens
International journal of biological macromolecules Porcine FcγRIIb mediated PRRSV ADE infection through inhibiting IFN-β by cytoplasmic inhibitory signal transduction Pubmed: 31284005
Journal of virology Marek's Disease Virus RLORF4 Inhibits Type I Interferon Production by Antagonizing NF-κB Activation Pubmed: 31243133
The Journal of Latvian Academy of Sciences Serodiagnosis of Human Bocavirus 1 Infection among Hospitalised Children with Lower Respiratory Tract Infection in Latvia
Cancer Medicine The immunostimulatory effects and pro‐apoptotic activity of rhCNB against Lewis lung cancer is mediated by Toll‐like receptor 4 Pubmed: 31218844
FASEB Journal Scavenger receptor A impairs interferon response to HBV infection by limiting TRAF 3 ubiquitination through recruiting OTUB 1
PLOS Pathogens Avian oncogenic herpesvirus antagonizes the cGAS-STING DNA-sensing pathway to mediate immune evasion Pubmed: 31539404
Autophagy Induction of autophagy and suppression of type I IFN secretion by CSFV Pubmed: 32160078
MOLECULAR IMMUNOLOGY BVDV-1 induces interferon-beta gene expression through a pathway involving IRF1, IRF7, and NF-κB activation Pubmed: 33053462
Autophagy LDHB inhibition induces mitophagy and facilitates the progression of CSFV infection Pubmed: 32924761
Arch Virol . Infectious bronchitis virus inhibits activation of the TLR7 pathway, but not the TLR3 pathway Pubmed: 32532134
Virulence Determination of antiviral action of Long Non-coding RNA loc107051710 During Infectious Bursal Disease Virus Infection due to Enhancement of Interferon …
bioRxiv Transferrin-dependent crosstalk between the intestinal tract and commensal microbes contributes for immune tolerance
Res Vet Sci Open reading frame 5 protein of porcine circovirus type 2 induces RNF128 (GRAIL) which inhibits mRNA transcription of interferon-β in porcine epithelial cells 34416463
Eur J Immunol Anti‐IFN‐α/‐ω neutralizing antibodies from COVID‐19 patients correlate with downregulation of IFN response and laboratory biomarkers of disease severity Pubmed:35419822
ResearchSquare Chicken Intestinal Microbiota Modulation of Resistance to Nephropathogenic Infectious Bronchitis Virus Infection Through IFN-I
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
APA222Ra02 Active Interferon Beta (IFNb) Cell culture; Activity Assays.
RPA222Ra02 Recombinant Interferon Beta (IFNb) Positive Control; Immunogen; SDS-PAGE; WB.
RPA222Ra01 Recombinant Interferon Beta (IFNb) Positive Control; Immunogen; SDS-PAGE; WB.
PAA222Ra01 Polyclonal Antibody to Interferon Beta (IFNb) WB; IHC; ICC; IP.
MAA222Ra21 Monoclonal Antibody to Interferon Beta (IFNb) WB; IHC; ICC; IP.
SEA222Ra ELISA Kit for Interferon Beta (IFNb) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA222Ra Multiplex Assay Kit for Interferon Beta (IFNb) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.