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Recombinant Matrix Metalloproteinase 9 (MMP9)

GELB; Gelatinase B; CLG4B; CLG4-B; 92 KDa Gelatinase; 92kDa Type IV Collagenase

  • Product No.RPA553Ca01
  • Organism SpeciesCanis familiaris; Canine (Dog) Same name, Different species.
  • SourceProkaryotic expression
  • HostE.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationSecreted, Extracellular matrix
  • Predicted Molecular Mass22.1kDa
  • Accurate Molecular Mass24kDa(Analysis of differences refer to the manual)
  • Residues & TagsAla225~Asp390 with N-terminal His Tag
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder
  • Purity> 90%
  • Isoelectric Point4.5
  • Applications Positive Control; Immunogen; SDS-PAGE; WB.
    If bio-activity of the protein is needed, please check active protein.
  • DownloadInstruction Manual
  • UOM 10µg50µg 200µg 1mg 5mg
  • FOB US$ 164  US$ 410  US$ 820  US$ 2460  US$ 6150 
    For more details, please contact local distributors!

SEQUENCE

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Journal of Bone and Joint Surgery Experimental osteoarthritis induced by surgical realignment of the patella in BALB/c mice PubMed: 21119180
Journal of Surgical Research Serum Levels of Matrix Metalloproteinase-2 as a Marker of Intimal Hyperplasia ScienceDirect: S0022480409001942
Journal of Proteome Research The Biological Behaviors of Rat Dermal Fibroblasts Can Be Inhibited by High Levels of MMP9 ACS: pr2006395
Zahedan Journal of Research in Medical Sciences The Effect of Acute Sub-Maximal Endurance Exercise on Serum Angiogenic Indices in Sedentary Men Zjrms: Source
Experimental & Translational Stroke Medicine Early outcome and blood-brain barrier integrity after co-administered thrombolysis and hyperbaric oxygenation in experimental stroke PubMed: 21679435
PLoS One Downregulation of CD9 in Keratinocyte Contributes to Cell Migration via Upregulation of Matrix Metalloproteinase-9 PubMed: PMC3797697
PLoS ONE A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches Plosone: Source
Rheumatology International Hypothermia induced by adenosine 5′-monophosphate attenuates early stage injury in an acute gouty arthritis rat model Pubmed: 23408150
Clinical and Investigative Medicine The protective effect of dexmedetomidine on bupivacaine-induced sciatic nerve inflammation is mediated by mast cells Pubmed: 23544611
Acta Histochemica RNA interference-mediated knockdown of Notch-1 inhibits migration and invasion, down-regulates matrix metalloproteinases and suppresses NF-κB signaling pathway in trophoblast cells Pubmed: 24681113
International journal of colorectal disease Expression and inhibition of matrix metalloproteinase (MMP)-8, MMP-9 and MMP-12 in early colonic anastomotic repair Pubmed: 23619615
Journal of Periodontology Effects of Photodynamic Therapy on the Clinical and Gingival Crevicular Fluid Inflammatory Biomarkers in Chronic Periodontitis: A Split-Mouth Randomized Clinical Trial Joponline: 130464
Polski Przeglad Chirurgiczny Concentration of gelatinases and their tissue inhibitors in pancreatic inflammatory and neoplastic tumors and their influence on the early postoperative course Pubmed: 23585206
Thromb Haemost. Analysis of the expression of nine secreted matrix metalloproteinases and their endogenous inhibitors in the brain of mice subjected to ischaemic stroke Pubmed:24671655
Int J Clin Exp Med. Celastrol may have an anti-atherosclerosis effect in a rabbit experimental carotid atherosclerosis model Pubmed:Pmc4132129
The Scientific World Journal Pass-predicted hepatoprotective activity of Caesalpinia sappan in thioacetamide-induced liver fibrosis in rats Pubmed:24701154
Journal of Perinatology Increased expression of prostasin contributes to early-onset severe preeclampsia through inhibiting trophoblast invasion Pubmed:25078863
World J Emerg Med Changes in biological behaviors of rat dermal fibroblasts induced by high expression of MMP9 Pubmed:25215164
PLOS ONE Melissa officinalis Extract Inhibits Laser-Induced Choroidal Neovascularization in a Rat Model Pubmed:25314292
Stem Cells International Matrix Metalloproteinase 9 Secreted by Hypoxia Cardiac Fibroblasts Triggers Cardiac Stem Cell Migration In Vitro Pubmed:25767513
Physiology and Pharmacology A Study on the Serum Levels of Angiogenic Factors in Response to Acute Long-term Submaximal Exercise in Sedentary Men Phypha:Source
Mediators of Inflammation Metalloproteinases and Their Tissue Inhibitors in Comparison between Different Chronic Pneumopathies in the Horse Journals: Mi
Journal of Biomaterials and Tissue Engineering MicroRNA-506 Up-Regulation Inhibits Growth and Invasion in Non-Small Cell Lung Cancer Cells by Possibly Targeting Sphingosine Kinase 1 Content: Asp
PLOS Adiponectin Attenuates Lung Fibroblasts Activation and Pulmonary Fibrosis Induced by Paraquat PubMed: 25945502
Biomedical Materials In vivo PubMed: 25634132
Histol Histopathol Estrogen-deficient osteoporosis enhances the recruitment and activity of osteoclasts by breast cancer cells PubMed: 26254457
Int J Clin Exp Pathol Up-regulation of microRNA-302a inhibited the proliferation and invasion of colorectal cancer cells by regulation of the MAPK and PI3K/Akt signaling pathways PubMed: 26191138
Hattori and Ishihara Stem Cell Research & Therapy Altered protein secretions during interactions between adipose tissue-or bone marrow-derived stromal cells and inflammatory cells PubMed: 25884474
Neuropeptides Melanocortin MC5 receptor agonists alleviate brain damage in abdominal compartment syndrome in the rat PubMed: 25616531
Veterinary Parasitology Expression of adhesion molecules, chemokines and matrix metallo- proteinases (MMPs) in viable and degenerating stage of Taenia solium metacestode in swine neurocysticercosis PubMed: 26412140
Canadian Journal of Physiology and Pharmacology Resveratrol reduces matrix metalloproteinases and alleviates intrahepatic cholestasis of pregnancy in rats Doi: Abs
PLASTIC AND AESTHETIC RESEARCH A comparative study to evaluate the effect of limited access dressing on diabetic ulcers Article: 2347-9264
Kyselov Влияние диуретиков с антиальдостероновым действием на состояние межклеточного матрикса и раннее постинфарктное ремоделирование левого … Article: View
Оригинальные исследования / Original researches матриксу та ранн Article: Viewfile
Int J Mol Sci Evodiamine Attenuates PDGF-BB-Induced Migration of Rat Vascular Smooth Muscle Cells through Activating PPARγ PubMed: 26703570
Deletion Polymorphism and Chronic Obstructive Pulmonary Disease ReCavia (Guinea pig )lation of Viral Infection-induced Airway Remodeling Cytokine Production by the TLR3-EGFR Signaling Pathway in Human Bronchial Epithelial Cells Pubmed:27075970
Basic Research CTGF upreCavia (Guinea pig )lation correlates with MMP-9 level in airway remodeling in a murine model of asthma Journal:27699
Journal of Cellular and Molecular Medicine Chronic myelogenous leukaemia exosomes modulate bone marrow microenvironment through activation of epidermal growth factor receptor Pubmed:27196940
Journal of Stroke and Cerebrovascular Diseases Epigallocatechin Gallate Extends the Therapeutic Window of Recombinant Tissue Plasminogen Activator Treatment in Ischemic Rats Pubmed:26851971
Journal of the Science of Food and Agriculture Actein protects against methylglyoxal-induced oxidative damage in osteoblastic MC3T3-E1 cells Pubmed:26991449
Cell Physiol Biochem. MicroRNA-340 Induces Apoptosis and Inhibits Metastasis of Ovarian Cancer Cells by Inactivation of NF-x03BA;B1 Pubmed:27160777
Int Wound J. From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases. Pubmed:26991748
Onco Targets Ther. sequential measurements of serum matrix metalloproteinase 9 to monitor chemotherapy responses in patients with advanced non-small-cell lung cancer pmc:PMC4898419
Am J Pathol.  Serum Osteopontin as a Novel Biomarker for Muscle Regeneration in Duchenne Muscular Dystrophy. Pubmed:26963343
Biochem Pharmacol. Gallium, a promising candidate to disrupt the vicious cycle driving osteolytic metastases. pubmed:27378505
disease markers Serum HMGB1 as a Potential Biomarker for Patients with Asbestos-Related Diseases. pubmed:28348451
Balkan Medical Journal Anti-inflammatory and Anti-apoptotic Effect of Valproic Acid and Doxycycline Independent from MMP Inhibition in Early Radiation Damage pubmed:27761275
SpringerPlus The effect of long-term amiodarone administration on myocardial fibrosis and evolution of left ventricular remodeling in a porcine model of ischemic … pubmed:27652141
Cerebrovascular Diseases Extra Increased Plasma Matrix Metalloproteinase-9 Levels Contribute to Intracerebral Hemorrhage during Thrombolysis after Concomitant Stroke and Influenza Infection pubmed:27560521
Microvascular Research Pleiotropic effects of survivin in vascular endothelial cells. pubmed:27373518
BMC Veterinary Research Metalloproteinases and their inhibitors are influenced by inhalative glucocorticoid therapy incombination with environmental dust reduction in equine recurrent airway obstruction. pubmed:27938355
Cellular Physiology and Biochemistry MicroRNA-340 Induces Apoptosis and Inhibits Metastasis of Ovarian Cancer Cells by Inactivation of NF-x03BA;B1. pubmed:27160777
Journal of Biomaterials and Tissue Engineering MicroRNA-340 Targets NF-κB1 to Inhibit Cell Proliferation, Migration and Invasion in Hepatocellular Carcinoma Cell Line publication:306067745
J Sci Food Agric. Actein protects against methylglyoxal-induced oxidative damage in osteoblastic MC3T3-E1 cells. pubmed:26991449
Sci Total Environ. The effects of particulate matter on inflammation of respiratory system: Differences betweenmale and female. pubmed:28174048
Journal of Biomaterials and Tissue Engineering Zoledronic Acid Inhibits TGF-β1-Induced the Proliferation, Migration and EMT Through Smad Pathway in Human Gingival Fibroblasts art00008
Human cell Liver X receptor α (LXRα) promoted invasion and EMT of gastric cancer cells by regulation of NF-κB activity pubmed:28091828
Human Cell MicroRNA-124 upregulation inhibits proliferation and invasion of osteosarcoma cells by targeting sphingosine kinase 1 pubmed:27743351
Experimental Parasitology Adhesion molecules, chemokines and matrix metallo-proteinases response after albendazole and albendazole plus steroid therapy in swine neurocysticercosis pubmed:28821422
Human Cell MicroRNA-30a functions as tumor suppressor and inhibits the proliferation and invasion of prostate cancer cells by down-regulation of SIX1 pubmed:28573504
Cellular Physiology and Biochemistry The MiR-495/Annexin A3/P53 Axis Inhibits the Invasion and EMT of Colorectal Cancer Cells pubmed:29224019
Evidence-Based Complementary and Alternative Medicine Cardioprotection against Heart Failure by Shenfu Injection via TGF-β/Smads Signaling Pathway pubmed:28698735
International Wound Journal From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases pubmed:26991748
Fundamental & Clinical Pharmacology Epigallocatechin-3-gallate ameliorates intrahepatic cholestasis of pregnancy by inhibiting matrix metalloproteinase-2 and matrix metalloproteinase-9 DOI:10.1111/fcp.12294
Scientific Reports Alantolactone induces apoptosis, promotes STAT3 glutathionylation and enhances chemosensitivity of A549 lung adenocarcinoma cells to doxorubicin via oxidative stress doi:10.1038/s41598-017-06535-y
Scientific Reports Amphiregulin contained in NSCLC-exosomes induces osteoclast differentiation through the activation of EGFR pathway pubmed:28600504
Archives of Medical Science CTGF upregulation correlates with MMP-9 level in airway remodeling in a murine model of asthma. pubmed:28507585
Molecular Medicine Reports Lysyl oxidase is involved in synovial hyperplasia and angiogenesis in rats with collagen‑induced arthritis pubmed:28901438
Surgical Innovation A Time Course of Bevacizumab (Anti-VEGF) Effect on Rat Peritoneum: Relations Between Antiadhesive Action and Fibrin Regulation Enzymes. pubmed:28877644
Journal of Neurochemistry Liraglutide ameliorated peripheral neuropathy in diabetic rats: Involvement of oxidative stress, inflammation and extracellular matrix remodeling Pubmed:29572844
Journal of Cellular Biochemistry Matrix metalloproteinases‐2 (MMP‐2) and matrix metalloproteinases‐9 (MMP‐9) are differentially expressed in different regions of normal and preeclampsia … 10.1002:jcb.26849
Inflammation Extracellular Matrix Remodeling and Modulation of Inflammation and Oxidative Stress by Sulforaphane in Experimental Diabetic Peripheral Neuropathy Pubmed:29704151
OncoTargets and Therapy sirT4 acts as a tumor suppressor in gastric cancer by inhibiting cell proliferation, migration, and invasion Pubmed:30022839
Biomed Research International CD30 Is Highly Expressed in Chronic Obstructive Pulmonary Disease and Induces the Pulmonary Vascular Remodeling Pubmed:29984229
Oncology Letters miRNA‑199a‑5p suppresses proliferation and invasion by directly targeting NF‑κB1 in human ovarian cancer cells 10.3892:ol.2018.9170
Taiwanese Journal of Obstetrics & Gynecology HMGB1-RAGE signaling pathway in pPROM Pubmed:29673663
Journal of Veterinary Science Clinical assessment after human adipose stem cell transplantation into dogs Pubmed:29284215
Life Sciences Inhibition of SRC/FAK cue: A novel pathway for the synergistic effect of rosuvastatin on the anti-cancer effect of dasatinib in hepatocellular carcinoma Pubmed: 30292831
Pediatric Research Anti-thymic stromal lymphopoietin antibody suppresses airway remodeling in asthma through reduction of MMP and CTGF. Pubmed: 30464333
Journal of cellular physiology Targeting peptidylarginine deiminase reduces neutrophil extracellular trap formation and tissue injury in severe acute pancreatitis Pubmed: 30515825
Clinical and experimental pharmacology & physiology A prospective, randomized trial of intravenous hydroxocobalamin versus norepinephrine or saline for treatment of lipopolysaccharide‐induced hypotension in a swine … Pubmed: 30575111
Journal of Ocular Pharmacology and Therapeutics Evaluation of the Effects of Biohcly in an Model of Mechanical Wounds in the Rabbit Cornea Pubmed: 30888249
Advanced Healthcare Materials Impact of Hydrogel Elasticity and Adherence on Osteosarcoma Cells and Osteoblasts Pubmed: 30838809
Cellular & Molecular Biology Letters MiR-613 inhibits proliferation and invasion and induces apoptosis of rheumatoid arthritis synovial fibroblasts by direct down-regulation of DKK1
ARCHIVES OF ORAL BIOLOGY Histopathological and Biochemical Evaluation of the effect of Paeoniflorin on the Periodontium During and After Periodontitis Formation in Rats Pubmed: 31005686
МАТРИКСНЫЕ МЕТАЛЛОПРОТЕИНАЗЫ: ЗНАЧЕНИЕ В РЕМОДЕЛИРОВАНИИ СЕРДЦА У ПАЦИЕНТОВ С ДИСПЛАЗИЕЙ СОЕДИНИТЕЛЬНОЙ ТКАНИ
Mediators of Inflammation Metalloproteinases and their Inhibitors under the Course of Immunostimulation by CPG-ODN and Specific Antigen Inhalation in Equine Asthma
International Journal of Clinical and Experimental Medicine Expression of MMP-9 and TIMP-1 in rat models of pressure ulcer and their significance
Antioxidants Hydroxytyrosol Supplementation Modifies Plasma Levels of Tissue Inhibitor of Metallopeptidase 1 in Women with Breast Cancer Pubmed: 31514476
Journal of Obstetrics and Gynaecology Research The levels of matrix metalloproteinase-9 and neutrophil gelatinase-associated lipocalin in different stages of endometriosis Pubmed: 31177884
Archives of Insect Biochemistry and Physiology Evaluation of the anti-atherogenic potential of Egyptian artichoke leaf extract in hypercholesterolemic rats Pubmed: 31566004
Medicina Proportion of the CD19-Positive and CD19-Negative Lymphocytes and Monocytes within the Peripheral Blood Mononuclear Cell Set Is Characteristic for Rheumatoid … Pubmed: 31554310
Oncology Research MicroRNA-6884-5p regulates the proliferation, invasion and EMT of gastric cancer cells by directly targeting S100A16 Pubmed: 31796150
Open Medicine Plasma matrix metalloproteinase-9 and tissue inhibitor of matrix metalloproteinase-1 as prognostic biomarkers in critically ill patients Pubmed: 32190734
Scientific Reports Matrix metallopeptidase expression and modulation by transforming growth factor-β1 in equine endometrosis Pubmed: 31980722
JOURNAL OF THROMBOSIS AND THROMBOLYSIS Extracellular matrix collagen biomarkers levels in patients with chronic thromboembolic pulmonary hypertension Pubmed: 33175289
Eur J Pharmacol TLR3 inhibitor and tyrosine kinase inhibitor attenuate cigarette smoke/poly I: C-induced airway inflammation and remodeling by the EGFR/TLR3/MAPK … Pubmed: 33068589
Cancers Multiple Myeloma-Derived Extracellular Vesicles Induce Osteoclastogenesis through the Activation of the XBP1/IRE1α Axis Pubmed: 32759820
Lab Invest Extracellular cold-inducible RNA-binding protein regulates neutrophil extracellular trap formation and tissue damage in acute pancreatitis Pubmed: 32709888
International Journal of Environmental Research and Public Health Biomarkers Facilitate the Assessment of Prognosis in Critically Ill Patients with Primary Brain Injury: A Cohort Study Pubmed: 32575870
J Cell Physiol A novel role of VEPH1 in regulating AoSMC phenotypic switching Pubmed: 32342520
JAMA Surgery Intraoperative dexmedetomidine for prevention of postoperative delirium in elderly patients with mild cognitive impairment Pubmed: 28593326
OMICS-A JOURNAL OF INTEGRATIVE BIOLOGY Intracranial Aneurysm Biomarker Candidates Identified by a Proteome-Wide Study Pubmed: 32525733
Advances in Animal and Veterinary Sciences Clinicopathological studies and new markers for trypanosoma evansi in experimen-tally infected rats
International Journal of Pharmacology Anti-arthritic Effects of Platelets Rich Plasma and Hyaluronic Acid on Adjuvant-induced Arthritis in Rats
Asian Journal of Pharmacy and Pharmacology Evaluation of altered ground matrix and matrix metalloproeinase (MMP'S) in wound healing with Aloe vera extract
Veterinary Sciences: Research and Reviews Investigation of matrix metalloproteinase-2 and matrix metalloproteinase-9 activity in some inflammatory conditions in Barki sheep
Identification of Polyphenolic Compounds and Hepatoprotective Activity of Artichoke (Cynara Scolymus L.) Edible Part Extracts in Rats
Клиническая медицина ДИНАМИКА МАРКЕРА ФИБРОЗИРОВАНИЯ ММР-9 У БОЛЬНЫХ ЛИМФОМОЙ ХОДЖКИНА
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH Krill oil and low-dose aspirin combination mitigates experimentally induced silicosis in rats: role of NF-¦ÊB/TGF-¦Â1/MMP-9 pathway 33398741
Oncol Lett GATA3 is downregulated in HCC and accelerates HCC aggressiveness by transcriptionally inhibiting slug expression 33613720
CLINICAL SCIENCE Berberine accelerated wound healing by restoring TrxR1/JNK in diabetes 33491733
Sci Rep Association between MIF gene promoter rs755622 and susceptibility to coronary artery disease and inflammatory cytokines in the Chinese Han population 33850223
MOLECULAR VISION Quantification of a panel for dry-eye protein biomarkers in tears: A comparative pilot study using standard ELISA and customized microarrays 34012227
Journal of Pregnancy Matrix Metalloproteinases MMP-2 and MMP-9 Occupy a New Role in Severe Preeclampsia 33381317
Stem Cells International The Paracrine Effect of Adipose-Derived Stem Cells Orchestrates Competition between Different Damaged Dermal Fibroblasts to Repair UVB-Induced Skin?¡­ 33381190
DOCSBAY Intermittent Pacing Therapy Favorably Modulates Infarct Remodeling 28386775
Drug Chem Toxicol Study of cardioprotective activity of the methanolic extract of the aerial parts of Bauhinia madagascariensis compared to Bauhinia purpurea against adrenaline … 34167392
JOURNAL OF FLUORESCENCE Time-resolved Fluorescence Immunoassay (TRFIA) for the Simultaneous Detection of MMP-9 and Lp-PLA2 in Serum 34495467
CNS Neurosci Ther Alterations in the gut microbiome with hemorrhagic transformation in experimental stroke 34591349
Calcium Binding Protein S100A16 Expedites Proliferation, Invasion and EMT Process in Gastric Cancer 34650982
Disease Markers Urinary Matrix Metalloproteinase-9 and Nephrin in Idiopathic Membranous Nephropathy: A Cross-Sectional Study 34707724
Medicina (Kaunas) Plasma Concentrations of New Biochemical Markers of Atherosclerosis in Patients with Dyslipidemia—A Pilot Study Pubmed:35743980
Biomolecules Increase in Serum MMP-9 and TIMP-1 Concentrations during Alcohol Intoxication in Adolescents—A Preliminary Study Pubmed:35625637
Journal of Pharmaceutical Research International Concomitant Administration of Rosuvastatin and Lefleunamide in Low doses Synergize Against Complete Freund's Adjuvant (CFA)-Induced Rheumatoid …
Non-POU Domain-Containing Octamer-Binding (NONO) Protein Stability Regulated by PIN1 is Crucial for Breast Cancer Tumorigenicity Via the MAPK/β …
Turkish Journal of Biochemistry-Turk Biyokimya Dergisi Downregulation of MMP-2 and MMP-9 genes in obesity patients and their relation with obesity-related phenotypes
Catalog No. Related products for research use of Canis familiaris; Canine (Dog) Organism species Applications (RESEARCH USE ONLY!)
RPA553Ca01 Recombinant Matrix Metalloproteinase 9 (MMP9) Positive Control; Immunogen; SDS-PAGE; WB.
PAA553Ca01 Polyclonal Antibody to Matrix Metalloproteinase 9 (MMP9) WB; IHC; ICC; IP.
MAA553Ca21 Monoclonal Antibody to Matrix Metalloproteinase 9 (MMP9) WB; IHC; ICC; IP.
SEA553Ca ELISA Kit for Matrix Metalloproteinase 9 (MMP9) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA553Ca Multiplex Assay Kit for Matrix Metalloproteinase 9 (MMP9) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.