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Recombinant Macrophage Migration Inhibitory Factor (MIF)

GIF; GLIF; MMIF; Glycosylation-Inhibiting Factor; L-dopachrome isomerase; L-dopachrome tautomerase; Phenylpyruvate tautomerase

  • Product No.RPA698Ra01
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • SourceProkaryotic expression
  • HostE.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationSecreted, Cytoplasm
  • Predicted Molecular Mass13.3kDa
  • Accurate Molecular Mass14kDa(Analysis of differences refer to the manual)
  • Residues & TagsPhe4~Ser112 with N-terminal His Tag
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder
  • Purity> 97%
  • Isoelectric Point6.7
  • Applications Positive Control; Immunogen; SDS-PAGE; WB.
    If bio-activity of the protein is needed, please check active protein.
  • DownloadInstruction Manual
  • UOM 10µg50µg 200µg 1mg 5mg
  • FOB US$ 196  US$ 490  US$ 980  US$ 2940  US$ 7350 
    For more details, please contact local distributors!

SEQUENCE

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Kidney International Targeted reduction of advanced glycation improves renal function in obesity PubMed: 21412218
The Journal of Immunology Macrophage Migration Inhibitory Factor Plays a Role in the Regulation of Microfold (M) Cell-Mediated Transport in the Gut Jimmunol: 5673
The Journal of Endocrinology Involvement of exercise-induced macrophage migration inhibitory factor in the prevention of fatty liver disease PubMed: PMC3757527
The Journal of Immunology Role of macrophage migration inhibitory factor in the regulatory T cell response of tumor-bearing mice PubMed: PMC3466372
Diabetologia Deletion of bone-marrow-derived receptor for AGEs (RAGE) improves renal function in an experimental mouse model of diabetes Pubmed:24957662
BioMed Research International The Potential Role of Polymethyl Methacrylate as a New Packaging Material for the Implantable Medical Device in the Bladder PubMed: 25705692
Diabetologia Combined NOX1/4 inhibition with GKT137831 in mice provides dose-dependent reno- and atheroprotection even in established micro- and macrovascular disease. pubmed:28160092
Life Sciences Protective effect of chlorogenic acid on the inflammatory damage of pancreas and lung in mice with l-arginine-induced pancreatitis pubmed:28919396
Scientific Reports Cytokine MIF Enhances Blood-Brain Barrier Permeability: Impact for Therapy in Ischemic Stroke Pubmed:29335619
Ocular Immunology and Inflammation Immune Response and Mechanisms of IFN-γ in Administration for Keratomycosis Pubmed: 30307777
International Journal of Immunopathology and Pharmacology Effect of voluntary running on expression of myokines in brains of rats with depression Pubmed: 30834799
Biomarkers in Heart Failure and Associated Diseases Biomarkers in Heart Failure and Associated Diseases
Science China-Life Sciences Autophagy mediates the secretion of macrophage migration inhibitory factor from cardiomyocytes upon serum-starvation Pubmed: 31209799
Folia Morphologica Beneficial effects of voluntary over forced exercise on skeletal muscle structure and myokines expression Pubmed: 31802473
Biomed Pharmacother Deletion of macrophage migration inhibitory factor ameliorates inflammation in mice model severe acute pancreatitis Pubmed: 32062385
Sci Rep Association between MIF gene promoter rs755622 and susceptibility to coronary artery disease and inflammatory cytokines in the Chinese Han population 33850223
Commun Biol Macrophage migration inhibitory factor is overproduced through EGR1 in TET2low resting monocytes Pubmed:35115654
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPA698Ra01 Recombinant Macrophage Migration Inhibitory Factor (MIF) Positive Control; Immunogen; SDS-PAGE; WB.
PAA698Ra01 Polyclonal Antibody to Macrophage Migration Inhibitory Factor (MIF) WB; IHC; ICC; IP.
MAA698Ra21 Monoclonal Antibody to Macrophage Migration Inhibitory Factor (MIF) WB; IHC; ICC; IP.
WEA698Ra Wide-range ELISA Kit for Macrophage Migration Inhibitory Factor (MIF) Enzyme-linked immunosorbent assay for Antigen Detection.
SEA698Ra ELISA Kit for Macrophage Migration Inhibitory Factor (MIF) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA698Ra Multiplex Assay Kit for Macrophage Migration Inhibitory Factor (MIF) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.