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Recombinant Glucose 6 Phosphate Isomerase (GPI)

AMF; NLK; PHI; SA36; Phosphoglucose Isomerase; Autocrine motility factor; Neuroleukin; Phosphohexose isomerase; Sperm antigen 36

  • Product No.RPA725Hu01
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • SourceProkaryotic expression
  • HostE.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationSecreted, Cytoplasm
  • Predicted Molecular Mass28kDa
  • Accurate Molecular Mass26kDa(Analysis of differences refer to the manual)
  • Residues & TagsMet263~Asn475 with N-terminal His Tag
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder
  • Purity> 90%
  • Isoelectric Point7.9
  • Applications Positive Control; Immunogen; SDS-PAGE; WB.
    If bio-activity of the protein is needed, please check active protein.
  • DownloadInstruction Manual
  • UOM 10µg50µg 200µg 1mg 5mg
  • FOB US$ 155  US$ 388  US$ 776  US$ 2328  US$ 5820 
    For more details, please contact local distributors!

SEQUENCE

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Oncology Reports Hyperthermia reduces migration of osteosarcoma by suppression of autocrine motility factor PubMed: 23027359
Arthritis & Rheumatology Bruton's tyrosine kinase deficiency inhibits autoimmune arthritis but fails to block immune complex‐mediated inflammatory arthritis Pubmed:26945549
Rheumatoid Arthritis Bruton's Tyrosine Kinase Deficiency Inhibits Autoimmune Arthritis in Mice but Fails to Block Immune Complex–Mediated Inflammatory Arthritis pubmed:26945549
Infection and Immunity The GPI transamidase complex subunit PbGPI16 of Plasmodium berghei is important for inducing experimental cerebral malaria Pubmed:29784863
Journal of Proteomics Proteomics analysis of asthenozoospermia and identification of glucose-6-phosphate isomerase as an important enzyme for sperm motility Pubmed: 31394311
Scientific Reports The Asthma-associated PER1-like domain-containing protein 1 (PERLD1) Haplotype Influences Soluble Glycosylphosphatidylinositol Anchor Protein (sGPI … Pubmed: 31959860
JOURNAL OF IMMUNOLOGY T–B Lymphocyte Interactions Promote Type 1 Diabetes Independently of SLAM-Associated Protein Pubmed: 33199538
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPA725Hu01 Recombinant Glucose 6 Phosphate Isomerase (GPI) Positive Control; Immunogen; SDS-PAGE; WB.
PAA725Hu01 Polyclonal Antibody to Glucose 6 Phosphate Isomerase (GPI) WB; IHC; ICC; IP.
LAA725Hu91 HRP-Linked Polyclonal Antibody to Glucose 6 Phosphate Isomerase (GPI) WB; IHC; ICC.
MAA725Hu22 Monoclonal Antibody to Glucose 6 Phosphate Isomerase (GPI) WB; IHC; ICC; IP.
SEA725Hu ELISA Kit for Glucose 6 Phosphate Isomerase (GPI) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA725Hu Multiplex Assay Kit for Glucose 6 Phosphate Isomerase (GPI) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.