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Recombinant Endothelial NOS (eNOS)

NOS3; ECNOS; NOS III; E-NOS; C-NOS; ENOS; CNOS; Constitutive NOS; Nitric Oxide Synthase 3, Endothelial

  • Product No.RPA868Ca01
  • Organism SpeciesCanis familiaris; Canine (Dog) Same name, Different species.
  • SourceProkaryotic expression
  • HostE.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationMembrane, Cytoplasm, Golgi apparatus
  • Predicted Molecular Mass24.0kDa
  • Accurate Molecular Mass25kDa(Analysis of differences refer to the manual)
  • Residues & TagsAla522~Phe705 with N-terminal His Tag
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder
  • Purity> 97%
  • Isoelectric Point6.8
  • Applications Positive Control; Immunogen; SDS-PAGE; WB.
    If bio-activity of the protein is needed, please check active protein.
  • DownloadInstruction Manual
  • UOM 10µg50µg 200µg 1mg 5mg
  • FOB US$ 196  US$ 490  US$ 980  US$ 2940  US$ 7350 
    For more details, please contact local distributors!

SEQUENCE

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Journal of Ethnopharmacology Impact of matrine on inflammation related factors in rat intestinal microvascular endothelial cells ScienceDirect: S0378874109004541
PLoS One A Novel Mechanism of Formaldehyde Neurotoxicity: Inhibition of Hydrogen Sulfide Generation by Promoting Overproduction of Nitric Oxide PubMed: 23359814
6th International Scientific Interdisciplinary Conference (ISIC) Inflammation influence on the formation of endothelial dysfunction in hypertensive patients caused by obesity Knmu: Source
PLoS One. SIRT1 Gene Polymorphisms Affect the Protein Expression in Cardiovascular Diseases Plos: Source
INTERNATIONAL JOURNAL OF MEDICINE AND MEDICAL RESEARCH L-ARGININE, BUT NOT L-NAME PROTECTS AGAINST LIVER INJURY INDUCED BY EXPERIMENTAL ISCHEMIA-REPERFUSION Tdmu:Source
Journal of Clinical Laboratory Analysis Soluble Lectin-Like Oxidized LDL Receptor 1 as a Possible Mediator of Endothelial Dysfunction in Patients With Metabolic Syndrome Pubmed:24798146
Endocrine Chemerin plays a protective role by regulating human umbilical vein endothelial cell-induced nitric oxide signaling in preeclampsia Pubmed:24840719
PLOS ONE A Remarkable Age-Related Increase in SIRT1 Protein Expression against Oxidative Stress in Elderly: PubMed: 25785999
J Trace Elem Med Biol Comparative angioprotective effects of magnesium compounds PubMed: 25127069
Journal of Functional Foods Peptides from hydrolysate of lantern fish (Benthosema pterotum) proved neuroprotective in vitro and in vivo science:S1756464616300639
Медицина и здравоохранение Роль индуцибельной NO-синтазы в механизмах регуляции функциональной активности кальций-активируемых калиевых каналов коронарных сосудов у крыс, адаптированных к стрессу article:n
journal of molecular and cellular cardiology Mutant DD genotype of NFKB1 gene is associated with the susceptibility and severity of coronary artery disease. pubmed:28088561
International Journal of Medicine and Medical Research ThE REmOdELINg Of LEfT ATRIUm ANd vAsOdILATION fAcTORs chANgEs IN IschEmIc hEART fAILURE 7038
International Journal of Ophthalmology Endothelial nitric oxide synthase deficiency influences normal cell cycle progression andapoptosis in trabecular meshwork cells. pubmed:27366677
Biomed Pharmacother. SIRT1 inhibits releases of HMGB1 and HSP70 from human umbilical vein endothelial cells caused by IL-6 and the serum from a preeclampsia patient and protects the cells from death. pubmed:28122311
Biomedicine & Pharmacotherapy SIRT1 inhibits releases of HMGB1 and HSP70 from human umbilical vein endothelial cells caused by IL-6 and the serum from a preeclampsia patient and protects the cells from death DOI:10.1016/j.biopha.2017.01.087
Asian Journal of Science and Technology THE INFLUENCE OF THE ADDITIONAL USAGE OF L-ARGININE ON THE FACTORS OF VASODILATION AND THE CLINICAL DATA, COMPLICATED WITH CARDIAC DECOMPENSATION WITH REDUCED AND PRESERVED FRACTURE OF THE LEFT VENTRICLE THAT PATIENTS WITH STABLE CORONARY HEART DISEASE HAVE ISSN: 0976-3376
National Science Review Blunted Nitric Oxide Regulation in Tibetans under High Altitude Hypoxia 10.1093:nsr
Biomedicine & Pharmacotherapy Molecular basis of vascular damage caused by cigarette smoke exposure and a new approach to the treatment: Alpha-linolenic acid Pubmed:29579706
Experimental and Therapeutic Medicine Ergothioneine prevents endothelial dysfunction induced by mercury chloride Pubmed:29805489
Turk Kardiyol Dern Ars.  Effect of statins on sirtuin 1 and endothelial nitric oxide synthase expression in young patients with a history of premature myocardial infarction Pubmed:29664427
Stężenia śródbłonkowej i indukowalnej formy syntazy tlenku azotu w surowicy krwi kobiet ciężarnych palących tytoń–badania wstępne
The Eurasian Journal of Medicine Tadalafil Preserves Penile Nitric Oxide Synthase from Detrimental Effect of Paroxetine in Rats Doi: 10.5152/eurasianjmed.2018.18160
International Journal of Environmental Research and Public Health Influence of Active Exposure to Tobacco Smoke on Nitric Oxide Status of Pregnant Women Pubmed: 30513890
Journal of Physics D: Applied Physics Premature Myocardial Infarction: Genetic Variations in SIRT1 Affect Disease Susceptibility
Particle and Fibre Toxicology Silica nanoparticles trigger the vascular endothelial dysfunction and prethrombotic state via miR-451 directly regulating the IL6R signaling pathway Pubmed: 30975181
ВЛИЯНИЕ ИНДУЦИБЕЛЬНОЙ NO-СИНТАЗЫ НА ТОНУС КОРОНАРНЫХ СОСУДОВ, СОКРАТИТЕЛЬНУЮ ФУНКЦИЮ МИОКАРДА И МЕХАНИЗМЫ …
Динамика показателей эндотелиальной дисфункции до и после операции у больных варикозной болезнью нижних конечностей
European Journal of Pharmacology The placental growth factor attenuates intimal hyperplasia in vein grafts by improving endothelial dysfunction Pubmed: 31836533
Rheumatol Int Circadian fluctuations of endothelial nitric oxide synthase activity in females with rheumatoid arthritis: a pilot study Pubmed: 32025851
Reviews on Recent Clinical Trials Circadian rhythms of endothelial nitric oxide synthase and toll-like receptors 2 production in females with rheumatoid arthritis depending on NOS3 gene polymorphism … Pubmed: 32297585
Georgian medical news ASSOCIATIONS BETWEEN EFFICACY OF THE THERAPY AND CIRCADIAN FLUCTUATIONS OF ENDOTHELIAL NITRIC OXIDE SYNTHASE WITH TOLL-LIKE … Pubmed: 32242853
Zaporozhye medical journal Dynamics of endothelial dysfunction indices before and after operation in patients with varicose disease of the lower extremities
regional hemodynamics and microcirculation Benzimidazole Derivative, 2-ETH Prevents Poststressor Disorders of Coronary Vascular Tone and Myocardial Contractility
Оксидативный стресс и эндотелиальная дисфункция в развитии экспериментального радиоиндуцированного повреждения легких и их коррекция …
Reprod Biol Angiogenic factors measured in aspirated placental tissue between the 10+ 6 and 18+ 3 weeks of gestation 34798517
J Basic Clin Physiol Pharmacol Anti-atherosclerotic activity of aqueous extract of Ipomoea batatas (L.) leaves in high-fat diet-induced atherosclerosis model rats Pubmed:34986543
Eur Rev Med Pharmacol Sci Serum inducible and endothelial nitric oxide synthase in coronary artery disease patients with Type 2 Diabetes mellitus Pubmed:35647851
Evidence-Based Complementary and Alternative Medicine Mechanism of Fructus Mume Pills Underlying Their Protective Effects in Rats with Acetic Acid-Inducedulcerative Colitis via the Regulation of Inflammatory … Pubmed:35620404
Catalog No. Related products for research use of Canis familiaris; Canine (Dog) Organism species Applications (RESEARCH USE ONLY!)
RPA868Ca01 Recombinant Endothelial NOS (eNOS) Positive Control; Immunogen; SDS-PAGE; WB.
PAA868Ca01 Polyclonal Antibody to Endothelial NOS (eNOS) WB; IHC; ICC; IP.