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Recombinant Defensin Beta 1 (DEFb1)

B-DF1; DEFB1; BD1; DEF-B1; DEFB101; HBD1

  • Product No.RPB373Mu01
  • Organism SpeciesMus musculus (Mouse) Same name, Different species.
  • SourceProkaryotic expression
  • HostE.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationMembrane, Secreted
  • Predicted Molecular Mass8.9kDa
  • Accurate Molecular Mass10kDa(Analysis of differences refer to the manual)
  • Residues & TagsVal22~Ser69 with N-terminal His Tag
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder
  • Purity> 80%
  • Isoelectric Point7.7
  • Applications Positive Control; Immunogen; SDS-PAGE; WB.
    If bio-activity of the protein is needed, please check active protein.
  • DownloadInstruction Manual
  • UOM 10µg50µg 200µg 1mg 5mg
  • FOB US$ 128  US$ 320  US$ 640  US$ 1920  US$ 4800 
    For more details, please contact local distributors!

SEQUENCE

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Appl Environ Microbiol. Modulation of Porcine β-Defensins 1 and 2 upon Individual and Combined Fusarium Toxin Exposure in a Swine Jejunal Epithelial Cell Line Asm: Source
British Journal of Nutrition Identification of sociodemographic and clinical factors associated with the levels of human β-defensin-1 and human β-defensin-2 in the human milk of Han Chinese Pubmed: 24124699
International Journal of Dairy Technology Concentration characteristics of bovine β-defensin 1 and 2 in fresh bovine milk and infant formula Doi: 10.1111
Molecular Nutrition Food Research Green tea polyphenol epigallocatechin-3-gallate improves epithelial barrier function by inducing the productionof antimicrobial peptide pBD-1 and pBD-2 in monolayers of porcine intestinal epithelial IPEC-J2 cells. Pubmed:26991948
Frontiers in Microbiology Swine-Derived Probiotic Lactobacillus plantarum Inhibits Growth and Adhesion of Enterotoxigenic Escherichia coli and Mediates Host Defense Pubmed:29997590
International Immunopharmacology Caprylic acid and nonanoic acid upregulate endogenous host defense peptides to enhance intestinal epithelial immunological barrier function via histone deacetylase … Pubmed: 30342347
cyberleninka EFFECT OF NIOSOMAL ANTIMICROBIAL PEPTIDE HBD-1 ON THE HEALING RATE OF INFECTED WOUNDS IN RATS
Fundamental & clinical pharmacology Effect of antimicrobial peptides HNP‐1 and hBD‐1 on Staphylococcus aureus strains in vitro and in vivo Pubmed: 31313350
Cytokine Levels of pro-and anti-inflammatory cytokines in cystic fibrosis patients with or without gingivitis Pubmed: 31927460
Animals Fat Encapsulation Reduces Diarrhea in Piglets Partially by Repairing the Intestinal Barrier and Improving Fatty Acid Transport 33375218
Sci Rep Human beta defensin levels and vaginal microbiome composition in post-menopausal women diagnosed with lichen sclerosus 34362937
J Mol Histol Isolation, culture, and identification of ceruminous gland cells Pubmed:35113280
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPB373Mu02 Recombinant Defensin Beta 1 (DEFb1) Positive Control; Immunogen; SDS-PAGE; WB.
RPB373Mu01 Recombinant Defensin Beta 1 (DEFb1) Positive Control; Immunogen; SDS-PAGE; WB.
PAB373Mu01 Polyclonal Antibody to Defensin Beta 1 (DEFb1) WB; IHC; ICC; IP.
PAB373Mu02 Polyclonal Antibody to Defensin Beta 1 (DEFb1) WB; IHC; ICC; IP.
LAB373Mu71 Biotin-Linked Polyclonal Antibody to Defensin Beta 1 (DEFb1) WB; IHC; ICC.
SEB373Mu ELISA Kit for Defensin Beta 1 (DEFb1) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB373Mu Multiplex Assay Kit for Defensin Beta 1 (DEFb1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.