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Recombinant Cytochrome P450 27A1 (CYP27A1)

CP27; CTX; CYP27; Sterol 27-Hydroxylase,Cerebrotendinous Xanthomatosis; Vitamin D(3) 25-hydroxylase; 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol 27-hydroxylase

  • Product No.RPD538Hu01
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • SourceProkaryotic expression
  • HostE.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationMitochondrion
  • Predicted Molecular Mass87kDa
  • Accurate Molecular Mass87&35kDa(Analysis of differences refer to the manual)
  • Residues & TagsAla34~Cys531 with N-terminal His and GST Tag
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder
  • Purity> 80%
  • Isoelectric Point8.4
  • Applications Positive Control; Immunogen; SDS-PAGE; WB.
    If bio-activity of the protein is needed, please check active protein.
  • DownloadInstruction Manual
  • UOM 10µg50µg 200µg 1mg 5mg
  • FOB US$ 140  US$ 350  US$ 700  US$ 2100  US$ 5250 
    For more details, please contact local distributors!

SEQUENCE

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Medicine (Baltimore) Vitamin D serum level is associated with Child–Pugh score and metabolic enzyme imbalances, but not viral load in chronic hepatitis B patients pubmed:27399065
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPD538Hu01 Recombinant Cytochrome P450 27A1 (CYP27A1) Positive Control; Immunogen; SDS-PAGE; WB.
RPD538Hu02 Recombinant Cytochrome P450 27A1 (CYP27A1) Positive Control; Immunogen; SDS-PAGE; WB.
PAD538Hu01 Polyclonal Antibody to Cytochrome P450 27A1 (CYP27A1) WB; IHC; ICC; IP.
MAD538Hu21 Monoclonal Antibody to Cytochrome P450 27A1 (CYP27A1) WB; IHC; ICC; IP.
SED538Hu ELISA Kit for Cytochrome P450 27A1 (CYP27A1) Enzyme-linked immunosorbent assay for Antigen Detection.
LMD538Hu Multiplex Assay Kit for Cytochrome P450 27A1 (CYP27A1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.